Fumarase Specific Activity Assay Kit (ab110043)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
Overview
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Product name
Fumarase Specific Activity Assay Kit
See all Fumarase kits -
Detection method
Colorimetric -
Assay type
Enzyme activity -
Species reactivity
Reacts with: Rat, Cow, Human
Does not react with: Mouse -
Product overview
ab110043 (MS780) is a novel assay that uses a high affinity monoclonal capture antibody to selectively isolate fumarase (FH), which removes all other enzymes of the Krebs cycle and NAD+/NADH utilizing enzymes. After isolation and subsequent measurement of the enzyme's functional activity, the quantity of isolated FH is measured in the same well by adding a second monoclonal detector antibody, which is quantified using a colorimetric label (HRP). Both reactions take place in time-dependent manners proportional to the amount of enzyme captured in each well. By combining activity and quantity measurements, the enzyme's relative specific activity can be determined. Specific activity is useful for measuring up or down regulation of activity by site-specific modification or damage, and in response to specific inhibitors.
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Notes
ab110043 (MS780) is a novel assay that uses a high affinity monoclonal capture antibody to selectively isolate fumarase (FH), which removes all other enzymes of the Krebs cycle and NAD+/NADH utilizing enzymes. After isolation and subsequent measurement of the enzyme's functional activity, the quantity of isolated FH is measured in the same well by adding a second monoclonal detector antibody, which is quantified using a colorimetric label (HRP). Both reactions take place in time-dependent manners proportional to the amount of enzyme captured in each well. By combining activity and quantity measurements, the enzyme's relative specific activity can be determined. Specific activity is useful for measuring up or down regulation of activity by site-specific modification or damage, and in response to specific inhibitors.
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Platform
Microplate reader
Properties
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Storage instructions
Please refer to protocols. -
Components 96 tests 100X Detector Antibody 1 x 130µl 100X HRP Label 1 x 130µl 10X Blocking Solution 1 x 10ml 1X Base Buffer 1 x 25ml 2000X Malate dehydrogenase 1 x 20µl 200X betaNAD+ 1 x 250µl 200X Fumarate (1M) 1 x 250µl 20X Wash Buffer 1 x 25ml 96-well microplate (12 strips) 1 unit Coupler 1 x 250µl Extraction Buffer (ab260490) 1 x 15ml HRP Development Solution 1 x 12ml Reagent Dye 1 x 250µl -
Research areas
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Alternative names
- FH
- Fumarate hydratase, mitochondrial
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Database links
- Entrez Gene: 2271 Human
- SwissProt: P07954 Human
- SwissProt: P14408 Rat
Associated products
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Positive Controls
Images
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Sandwich ELISA - Fumarase Specific Activity Assay Kit (ab110043)Example data obtained using the Fumarase Specific Activity Assay Kit (ab110043).
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Sandwich ELISA - Fumarase Specific Activity Assay Kit (ab110043)Principle of a Sandwich ELISA.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab110043 has been referenced in 2 publications.
- Wang Z et al. A fungal ABC transporter FgAtm1 regulates iron homeostasis via the transcription factor cascade FgAreA-HapX. PLoS Pathog 15:e1007791 (2019). PubMed: 31545842
- Perrier-Trudova V et al. Fumarate Hydratase-deficient Cell Line NCCFH1 as a New In Vitro Model of Hereditary Papillary Renal Cell Carcinoma Type 2. Anticancer Res 35:6639-53 (2015). PubMed: 26637880