Fumarate Assay Kit (ab102516)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 40 min
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 1 nmol/well
Product nameFumarate Assay Kit
Sample typeCell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
Sensitivity> 1 nmol/well
Assay time0h 40m
Fumarate Assay Kit ab102516 provides a convenient tool for sensitive detection of Fumarate in biological samples.
In the fumarate assay protocol, the fumarate enzyme mix recognizes fumarate as a specific substrate leading to proportional color development. The amount of fumarate can therefore be easily quantified using a colorimetric assay (λ = 450 nm). It can detect as low as 1 nmol of fumarate per well (20 µM).
Fumurate assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 1 hr
- analyze with a microplate reader
This product is manufactured by BioVision, an Abcam company and was previously called K633 Fumarate Colorimetric Assay Kit. K633-100 is the same size as the 100 test size of ab102516.
Fumarate (HO2CCH=CHCO2H-) is an intermediate in the Kreb's cycle used by cells to metabolize food to form ATP. In the mammalian liver, Fumarate is also a product of the Urea cycle where its release in the cytosol leads to its conversion into malate and subsequently oxaloacetate while generating NADH in the cytosol.
Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 100 tests Fumarate Assay Buffer WM 1 x 25ml Fumarate Developer Red 1 vial Fumarate Enzyme Mix Green 1 vial Fumarate Standard Yellow 1 x 0.2ml
RelevanceFumarate (HO2CCH=CHCO2H-) is an intermediate in the Kreb’s cycle used by cells to metabolize food to form ATP. In the mammalian liver, Fumarate is also a product of the Urea cycle where its release in the cytosol leads to its conversion into malate and subsequently oxaloacetate while generating NADH in the cytosol. The human skin naturally produces fumaric acid when exposed to sunlight. In fact, fumaric acid esters have been used to treat psoriasis, possibly due to an impaired production of fumaric acid in the skin. Fumaric acid has also been used in beverages, baking powders and candy.
Fumurate Standard Curve
Fumarate measured in mouse tissue lysates (mg of extracted protein), background signal subtracted (duplicates +/- SD).
Fumarate measured in biological fluids, background signal subtracted (duplicates +/- SD).
Fumarate measured in cell culture medium and control medium, background signal subtracted (duplicates +/- SD).
Fumarate measured in cell lysates, background signal subtracted (duplicates +/- SD).
Datasheets and documents
ab102516 has been referenced in 18 publications.
- An W & Kang JS Effect of Metformin on Myocardial Injury Induced by Hepatic Ischemia-Reperfusion in Rats. Front Pharmacol 13:822743 (2022). PubMed: 35431970
- Singh V et al. The Molecular Role of HIF1a Is Elucidated in Chronic Myeloid Leukemia. Front Oncol 12:912942 (2022). PubMed: 35847841
- Chow HM et al. Low-Density Lipoprotein Receptor-Related Protein 6 Cell Surface Availability Regulates Fuel Metabolism in Astrocytes. Adv Sci (Weinh) 8:e2004993 (2021). PubMed: 34180138
- Aggarwal RK et al. Functional succinate dehydrogenase deficiency is a common adverse feature of clear cell renal cancer. Proc Natl Acad Sci U S A 118:N/A (2021). PubMed: 34551979
- Taha-Mehlitz S et al. Adenylosuccinate lyase is oncogenic in colorectal cancer by causing mitochondrial dysfunction and independent activation of NRF2 and mTOR-MYC-axis. Theranostics 11:4011-4029 (2021). PubMed: 33754045