Glucose-6-Phosphate Assay Kit (Colorimetric) (ab83426)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 40 min
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 10 µM
Product nameGlucose-6-Phosphate Assay Kit (Colorimetric)
See all Glucose 6 phosphate kits
Sample typeCell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
Range1 µM - 30 µM
Assay time0h 40m
Glucose-6-Phosphate Assay Kit (Colorimetric) (ab83426) is a simple, sensitive and rapid test to quantify glucose-6-phospate (G6P) in a variety of samples. In the assay, glucose-6-phosphate is oxidized into an intermediate product which in turn converts the probe into an intensely colored product with an absorbance at OD=450 nm. This product can detect G6P in the range of 1-30 nmoles with a detection sensitivity ~10 µM G6P.
For higher sensitivity, we recommend our fluorometric assay Glucose-6-Phosphate Assay Kit - High Sensitivity (Fluorometric) (ab107923).
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This product is manufactured by BioVision, an Abcam company and was previously called K657 Glucose-6-Phosphate Colorimetric Assay Kit. K657-100 is the same size as the 100 test size of ab83426.
Glucose-6-phosphate (G6P) is a key sugar intermediate for glucose to get into cells, and then enter either metabolic pathways or storage. G6P can enter the glycolytic pathway, the pentose phosphate shunt or be stored as glycogen or starch. G6P is transformed by G6PDH to generate reducing equivalents in the form of NADPH. This is particularly important in red blood cells where a G6PDH deficiency leads to hemolytic anemia.
Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 100 tests G6P Assay Buffer WM 1 x 25ml G6P Enzyme Mix (Lyophilised) Green 1 vial G6P Standard (10 µmol) (Lyophilised) Yellow 1 vial G6P Substrate Mix (Lyophilised) Red 1 vial
RelevanceGlucose 6 phosphate (G6P) is a key sugar intermediate for glucose to get into cells, and then enter either metabolic pathways or storage. G6P can enter the glycolytic pathway, the pentose phosphate shunt or be stored as glycogen or starch. G6P is utilized by its dehydrogenase to generate reducing equivalents in the form of NADPH. This is particularly important in red blood cells where a G6PDH deficiency leads to hemolytic anemia.
G6P (A) levels are elevated in GAA−/− mice compared to wild type (C57/Bl6) and reduced by rhGAA treatment.
GAA−/− mice were dosed with rhGAA. G6P was quantified in heart and triceps homogenates from wild type (C57Bl/6) and GAA−/− mice using ab83426. Values are means ± SEM. Data was analyzed by one-way ANOVA followed by Newman-Keuls comparing groups. ***P<0.001.
Colorimetric standard curve: mean of duplicates (+/- SD) with background reads subtracted.
Datasheets and documents
ab83426 has been referenced in 13 publications.
- Christofi M et al. Low-dose 2-deoxy glucose stabilises tolerogenic dendritic cells and generates potent in vivo immunosuppressive effects. Cell Mol Life Sci 78:2857-2876 (2021). PubMed: 33074350
- Schulz A et al. The stress-responsive gene GDPGP1/mcp-1 regulates neuronal glycogen metabolism and survival. J Cell Biol 219:N/A (2020). PubMed: 31968056
- Mann Brukner A et al. Effects of Valproic Acid on Cerebral Nutrient Carriers' Expression in the Rat. Front Pharmacol 9:1054 (2018). PubMed: 30298005
- Pursell N et al. Inhibition of Glycogen Synthase II with RNAi Prevents Liver Injury in Mouse Models of Glycogen Storage Diseases. Mol Ther N/A:N/A (2018). Functional Studies . PubMed: 29784585
- Tojo A et al. H+-ATPase blockade reduced renal gluconeogenesis and plasma glucose in a diabetic rat model. Med Mol Morphol 51:89-95 (2018). PubMed: 29318388