Glucose Assay Kit (Fluorometric, High Sensitivity) (ab169559)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Adherent cells, Other biological fluids, Plasma, Serum, Suspension cells, Tissue, Tissue Culture Media
- Sensitivity: 0.5 µM
Overview
-
Product name
Glucose Assay Kit (Fluorometric, High Sensitivity)
See all Glucose kits -
Detection method
Fluorescent -
Sample type
Serum, Plasma, Other biological fluids, Tissue, Adherent cells, Suspension cells, Tissue Culture Media -
Assay type
Quantitative -
Sensitivity
< 0.5 µM -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Picoprobe Glucose Assay Kit (ab169559) is a simple, rapid, ultra-sensitive assay to measure glucose. It is suitable for high-throughput use. In this assay, D-glucose is enzymatically oxidized to form a product which reacts with a colorless probe to generate the fluorescence (Ex/Em = 535/587 nm). The fluorescence generated is directly proportional to the amount of glucose. This assay kit can detect less than 0.5 µM glucose in various biological samples.
-
Notes
This product is manufactured by BioVision, an Abcam company and was previously called K688 PicoProbe™ Glucose Fluorometric Assay Kit. K688-100 is the same size as the 100 test size of ab169559.
Glucose is the main energy source for virtually all living organisms. Glucose level is a key diagnostic parameter for many metabolic disorders. Measurement of glucose can be very important in both research and drug discovery processes.
-
Platform
Microplate reader
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Glucose Assay Buffer 1 x 25ml Glucose Enzyme Mix (lyophilized) Green 1 vial Glucose Standard Yellow 1 x 100µl Glucose Substrate Mix Red 1 vial PicoProbe Blue 1 x 0.4ml -
Research areas
-
Relevance
Glucose (C6H12O6; FW: 180.16) is a ubiquitous energy source in most organisms, from bacteria to humans. The breakdown of carbohydrates produces mono- and disaccharides, most of which is glucose. Through glycolysis and TCA (citric acid cycle), glucose is oxidized to eventually form CO2 and water, generating the universal energy molecule ATP. Glucose is a primary source of energy for the brain and a critical component in the production of proteins and in lipid metabolism and therefore measurement of glucose level is a key diagnostic parameter for many metabolic disorders.
Images
-
Glucose standard curveRepresentative standard curve.
-
Measurement of glucose levels in samplesMeasurement of glucose levels in human serum (1 µL of 1:10 diluted) and rat tissue lysates from liver, kidney and muscle (0.14 µg, 0.19 µg and 0.93 µg respectively).
Datasheets and documents
-
SDS download
-
Datasheet download
References (9)
ab169559 has been referenced in 9 publications.
- Meng F et al. LncRNA LINC00525 activates HIF-1a through miR-338-3p / UBE2Q1 / ß-catenin axis to regulate the Warburg effect in colorectal cancer. Bioengineered 13:2554-2567 (2022). PubMed: 35156520
- Cai W et al. MITF-Mediated lncRNA CCDC183-As1 Promotes the Tumorigenic Properties and Aerobic Glycolysis of Bladder Cancer via Upregulating TCF7L2. J Oncol 2022:6785956 (2022). PubMed: 35957803
- Li L et al. LncRNA OIP5-AS1 Regulates the Warburg Effect Through miR-124-5p/IDH2/HIF-1a Pathway in Cervical Cancer. Front Cell Dev Biol 9:655018 (2021). PubMed: 34513821
- Tang CK et al. Real-time monitoring of deformed wing virus-infected bee foraging behavior following histone deacetylase inhibitor treatment. iScience 24:103056 (2021). PubMed: 34755080
- Timper K et al. GLP-1 Receptor Signaling in Astrocytes Regulates Fatty Acid Oxidation, Mitochondrial Integrity, and Function. Cell Metab 31:1189-1205.e13 (2020). PubMed: 32433922
- Schömel N et al. UGCG influences glutamine metabolism of breast cancer cells. Sci Rep 9:15665 (2019). PubMed: 31666638
- Wang L et al. Arylamine N-acetyltransferase 1 protects against reactive oxygen species during glucose starvation: Role in the regulation of p53 stability. PLoS One 13:e0193560 (2018). PubMed: 29518119
- Kuang X et al. The tumor suppressor gene lkb1 is essential for glucose homeostasis during zebrafish early development. FEBS Lett 590:2076-85 (2016). PubMed: 27264935
- Plauth A et al. Hormetic shifting of redox environment by pro-oxidative resveratrol protects cells against stress. Free Radic Biol Med 99:608-622 (2016). PubMed: 27515816