Glycerol-3-phosphate (G3P) Assay Kit (Colorimetric) (ab174094)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Cell Lysate, Tissue Lysate
- Sensitivity: 2 nmol/well
Overview
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Product name
Glycerol-3-phosphate (G3P) Assay Kit (Colorimetric)
See all glycerol-3-phosphate kits -
Detection method
Colorimetric -
Sample type
Cell Lysate, Tissue Lysate -
Assay type
Quantitative -
Sensitivity
< 2 nmol/well -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Abcam’s Glycerol-3-Phosphate Assay kit (Colorimetric) (ab174094) is a sensitive, fast and easy-to-use kit. In this assay, G3P is oxidized by G3P Enzyme Mix to form an intermediate, which reduces a nearly colorless probe to a colored product with strong absorbance at 450 nm. This assay kit can detect G3P less than 2 nmol/well and can be used for a variety of sample types.
Visit our FAQs page for tips and troubleshooting.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K641 Glycerol-3-Phosphate (G3P) Colorimetric Assay Kit. K641-100 is the same size as the 100 test size of ab174094.
Glycerol-3-phosphate (G3P) is an important intermediate for all living organisms. Glycerol-3-Phosphate is produced either by glycerol via glycerol kinase or by dihydroxyacetone phosphate through glycerol-3-phosphate dehydrogenase. In response to cellular signals, glycerol-3-phosphate can be utilized in multiple pathways: it can be further converted into glyceraldehyde-3-phosphate and enter glycolysis or rapidly generate NAD+ in brain or muscle tissues through the G3P shuttle or enter the lipid biosynthetic pathway. Recent studies have found that glycerol-3-phosphate is a novel regulator and plays a fundamental defense role in plant pathogenesis.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests G3P Assay Buffer 1 x 25ml G3P Enzyme Mix (Lyophilized) 1 vial G3P Probe 1 vial G3P Standard (Lyophilized) 1 vial -
Research areas
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Relevance
Glycerol-3-phosphate (G3P) is an important intermediate for all living organisms. Glycerol-3-Phosphate is produced either by glycerol via glycerol kinase or by dihydroxyacetone phosphate through glycerol-3-phosphate dehydrogenase. In response to cellular signals, glycerol- 3-phosphate can be utilized in multiple pathways: it can be further converted into glyceraldehyde-3-phosphate and enter glycolysis or rapidly generate NAD+ in brain or muscle tissues through the G3P shuttle or enter the lipid biosynthetic pathway. Recent studies have found that glycerol-3-phosphate is a novel regulator and plays a fundamental defense role in plant pathogenesis. -
Alternative names
- G3P
- glycerol 3 phosphate
- glycerol-3-phosphate
Images
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G3P Standard Curve
This is example data only.
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Measurement of G3P in various samplesMeasurement of G3P in rat liver (100 µg), 3T3-L1 (40 µg) and Hela (50 µg) lysate. This is example data.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab174094 has been referenced in 2 publications.
- Zhang W et al. Allosteric activation of the metabolic enzyme GPD1 inhibits bladder cancer growth via the lysoPC-PAFR-TRPV2 axis. J Hematol Oncol 15:93 (2022). PubMed: 35836291
- Xie J et al. GPD1 Enhances the Anticancer Effects of Metformin by Synergistically Increasing Total Cellular Glycerol-3-Phosphate. Cancer Res 80:2150-2162 (2020). PubMed: 32179514