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Signal Transduction Metabolism Amino Acids
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GSH Assay Kit (Colorimetric) (ab239727)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Submit a question References (3)

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GSH Standard Curve, results from multiple experimets.
  • GSH Standard Curve, results from multiple experimets.
  • Assay Specificity.
  • Total Collagen Assay Kit (Perchlorate-Free) (ab222942)

Key features and details

  • Detection method: Colorimetric
  • Platform: Microplate reader
  • Sample type: Cell Lysate, Tissue Homogenate

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Overview

  • Product name

    GSH Assay Kit (Colorimetric)
    See all Glutathione kits
  • Detection method

    Colorimetric
  • Sample type

    Cell Lysate, Tissue Homogenate
  • Product overview

    GSH Assay Kit (Colorimetric) (ab239727) provides a simple in vitro assay for detecting reduced GSH levels in apoptosis and other pathological processes. The Kit is based on an enzymatic cycling method in the presence of GSH and a chromophore. The reduction of the chromophore produces a stable product, which can be followed kinetically at 450 nm. Therefore, its absorbance is directly proportional to the amount of GSH in the sample.


    The assay is simple, reproducible and can specifically detect as low as 50 pmol/well of reduced form of Glutathione (GSH) in a 100 µl reaction.


    This assay specifically detects reduced glutathione. Oxidized GSSG is not detected.


     

  • Notes

    This product is manufactured by BioVision, an Abcam company and was previously called K464 Reduced Glutathione (GSH) Assay Kit (Colorimetric). K464-100 is the same size as the 100 test size of ab239727.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    Enzyme Mix A 1 x 15µl
    Enzyme Mix B 1 x 120µl
    Enzyme Mix C 1 vial
    GSH Assay Buffer 1 x 50ml
    GSH Standard 1 vial
    Substrate Mix A 1 x 1ml
    Substrate Mix B 1 vial
    Sulfosalicylic Acid (SSA, 1 gram) 1 unit
  • Research areas

    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Cell Biology
    • Other Antibodies
    • Oxidative Stress
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Oxidative stress
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Antioxidants
  • Relevance

    Glutathione is a small peptide composed of three amino acids: cysteine, glutamic acid, and glycine and is present in tissues in concentrations as high as one millimolar. It contains an unusual peptide linkage between the amine group of cysteine and the carboxyl group of the glutamate side chain. Glutathione is involved in detoxification, it binds to toxins, such as heavy metals, solvents, and pesticides, and transforms them into a form that can be excreted in urine or bile. It is also an important antioxidant, helping to maintain the -SH groups of proteins in their reduced form. Chronic functional glutathione deficiency is associated with glucose 6-phosphate dehydrogenase deficiency, immune disorders, an increased incidence of malignancies, and in the case of HIV disease, probably accelerated pathogenesis of the disease. Acute manifestations of functional glutathione deficiency can be seen in those who have taken an overdosage of acetaminophen (paracetamol). This results in depletion of glutathione in the hepatocytes, leading to liver failure and death.
  • Alternative names

    • Glutathione
    • GSH
    • Oxidised glutathione
    • Reduced glutathione

Images

  • GSH Standard Curve, results from multiple experimets.
    GSH Standard Curve, results from multiple experimets.
  • GSH Standard Curve, results from multiple experimets.
    GSH Standard Curve, results from multiple experimets.
  • Assay Specificity.
    Assay Specificity.

    Measurement of GSH (1 nmol) and GSSG (100 nmol). The assay kit can effectively discriminate between reduced GSH and oxidized GSSG forms.

  • Total Collagen Assay Kit (Perchlorate-Free) (ab222942)
    Total Collagen Assay Kit (Perchlorate-Free) (ab222942)

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (3)

Publishing research using ab239727? Please let us know so that we can cite the reference in this datasheet.

ab239727 has been referenced in 3 publications.

  • Li S  et al. Evaluation of hyperbranched polyglycerol for cold perfusion and storage of donor kidneys in a pig model of kidney autotransplantation. J Biomed Mater Res B Appl Biomater 109:853-863 (2021). PubMed: 33098184
  • Korzekwa AJ  et al. Antioxidative Potential of Red Deer Embryos Depends on Reproductive Stage of Hind as A Oocyte Donor. Animals (Basel) 10:N/A (2020). PubMed: 32674333
  • Tang W  et al. miR-455 targets FABP4 to protect human endometrial stromal cells from cytotoxicity induced by hydrogen peroxide. Mol Med Rep 20:4781-4790 (2019). PubMed: 31638263

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