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    products/assay-kits/hdl-uptake-assay-kit-fluorometric-ab204717.pdf

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Cardiovascular Lipids / Lipoproteins Lipid Metabolism Cholesterol Metabolism
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HDL Uptake Assay Kit (Fluorometric) (ab204717)

  • Datasheet
  • SDS
  • Protocol Booklet
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HDL Uptake Assay Kit (Fluorometric) (ab204717)
  • HDL Uptake Assay Kit (Fluorometric) (ab204717)

Key features and details

  • Assay type: Cell-based (quantitative)
  • Detection method: Fluorescent
  • Platform: Microplate reader
  • Sample type: Adherent cells, Suspension cells

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Overview

  • Product name

    HDL Uptake Assay Kit (Fluorometric)
    See all HDL kits
  • Detection method

    Fluorescent
  • Sample type

    Adherent cells, Suspension cells
  • Assay type

    Cell-based (quantitative)
  • Species reactivity

    Reacts with: Mammals, Other species
  • Product overview

    HDL Uptake Assay Kit (Fluorometric) (ab204717) contains a fluorescently-labeled HDL that can be measured when taken up by cells. Unlabeled-HDL is included in the kit for assay validation.

  • Notes

    This product is manufactured by BioVision, an Abcam company and was previously called K586 HDL Uptake Assay Kit (Fluorometric). K586-100 is the same size as the 100 test size of ab204717.

    High-density lipoprotein (HDL) consists of a protein shell, containing apolipoproteins A-I and A-II, and a hydrophobic core consisting of cholesterol and triglycerides surrounded by phospholipids and cholesterol esters. HDL transports cholesterol to the liver or steroidogenic organs such as adrenals, ovary, and testes by both direct and indirect pathways. HDL-Cholesterol is removed by HDL receptors such as scavenger receptor BI (SR-BI), which mediate the selective uptake of cholesterol from HDL. Clinically, high levels of HDL has been associated with cardiovascular health. This is due to the ability of HDL particle to transport cholesterol from lipid-laden macrophages of atherosclerotic arteries to the liver for secretion into the bile by a process called as reverse cholesterol transport.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    Assay Buffer 1 x 100ml
    Fluorescently-labeled HDL (5 mg/ml) 1 x 1ml
    Unlabeled HDL (2X) 1 x 100µl
  • Research areas

    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Cholesterol Metabolism
    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipoproteins/Apolipoproteins
    • Lipoproteins
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Cardiovascular
    • Atherosclerosis
    • Lipid transport
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Cholesterol Metabolism
  • Relevance

    High-density lipoprotein (HDL) is one of the five major groups of lipoproteins, which, in order of sizes, largest to smallest, are chylomicrons, VLDL, IDL, LDL, and HDL, which enable lipids like cholesterol and triglycerides to be transported within the water-based bloodstream. In healthy individuals, about thirty percent of blood cholesterol is carried by HDL.

    Blood tests typically report HDL-C level, i.e. the amount of cholesterol contained in HDL particles. It is often contrasted with low-density or LDL cholesterol or LDL-C. HDL particles are able to remove cholesterol from within artery atheroma and transport it back to the liver for excretion or re-utilization, which is the main reason why the cholesterol carried within HDL particles (HDL-C) is sometimes called "good cholesterol" (despite the fact that it is exactly the same as the cholesterol in LDL particles).

  • Alternative names

    • High Density Lipoprotein

Images

  • HDL Uptake Assay Kit (Fluorometric) (ab204717)
    HDL Uptake Assay Kit (Fluorometric) (ab204717)

    Fluorescently-labeled HDL Standard curve.

  • HDL Uptake Assay Kit (Fluorometric) (ab204717)
    HDL Uptake Assay Kit (Fluorometric) (ab204717)

    Fluorescently-labeled HDL uptake in HepG2 (hepatocytes) cells. Cells were starved for 4 hrs and incubated with Fluorescently-labeled HDL (10 µg) for 4 hrs. Fluorescently-labeled HDL (HDL) uptake was inhibited by SR-BI (HDL receptor) antibody (HDL + SRB1 Ab1), BLT1 (SR-BI Inhibitor; HDL + BLT1) and outcompeted by Unlabeled-HDL (HDL+ UL-HDL [20 µg]). Background was subtracted from the data (Wash off was subtracted as background).

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (4)

Publishing research using ab204717? Please let us know so that we can cite the reference in this datasheet.

ab204717 has been referenced in 4 publications.

  • Riscal R  et al. Cholesterol Auxotrophy as a Targetable Vulnerability in Clear Cell Renal Cell Carcinoma. Cancer Discov 11:3106-3125 (2021). PubMed: 34244212
  • Huang Y  et al. Long Noncoding RNA-H19 Contributes to Atherosclerosis and Induces Ischemic Stroke via the Upregulation of Acid Phosphatase 5. Front Neurol 10:32 (2019). PubMed: 30778327
  • Florijn BW  et al. Diabetic Nephropathy Alters the Distribution of Circulating Angiogenic MicroRNAs Among Extracellular Vesicles, HDL, and Ago-2. Diabetes 68:2287-2300 (2019). PubMed: 31506344
  • Zhu L  et al. Hepatocyte estrogen receptor alpha mediates estrogen action to promote reverse cholesterol transport during Western-type diet feeding. Mol Metab 8:106-116 (2018). PubMed: 29331506

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