For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    By product type
    Proteins and Peptides
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    products/assay-kits/hydrogen-peroxide-assay-kit-ab102500.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Kits/ Lysates/ Other Kits Cell Metabolism Kits Other Metabolism Assay
Share by email

Hydrogen Peroxide Assay Kit (ab102500)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Q&A (18)References (82)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Functional Studies - Hydrogen Peroxide Assay Kit (ab102500)
  • Functional Studies - Hydrogen Peroxide Assay Kit (ab102500)
  • Functional Studies - Hydrogen Peroxide Assay Kit (ab102500)

Key features and details

  • Assay type: Quantitative
  • Platform: Microplate reader
  • Assay time: 1 hr
  • Sample type: Cell culture media, Cell Lysate, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
  • Sensitivity: 0.04 µM

You may also be interested in

Assay
Product image
DCFDA / H2DCFDA - Cellular ROS Assay Kit (ab113851)
Assay
Product image
Catalase Activity Assay Kit (Immunocapture) (ab118184)
ELISA
Product image
Human alpha SMA ELISA Kit (ab240678)

View more associated products

Overview

  • Product name

    Hydrogen Peroxide Assay Kit
    See all Hydrogen Peroxide kits
  • Sample type

    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media
  • Assay type

    Quantitative
  • Sensitivity

    > 0.04 µM
  • Assay time

    1h 00m
  • Product overview

    Hydrogen Peroxide Assay Kit ab102500 is a highly sensitive, simple, direct and HTS-ready colorimetric and fluorometric assay for measuring H2O2 in biological samples.


    In the hydrogen peroxide assay protocol, horse radish peroxidase (HRP) reacts with a probe and H2O2 to produce a product with color (λmax = 570 nm) and red-fluorescence (Ex/Em=535/587 nm). 


    The detection limit is 2 pmol per assay (or 40 nM concentration) of H2O2 in the sensitive fluorometric assay.


    Hydrogen peroxide assay protocol summary:
    - add standards and samples to wells
    - add reaction mix and incubate for 10 min
    - analyze with microplate reader

  • Notes

    This product is manufactured by BioVision, an Abcam company and was previously called K265 Hydrogen Peroxide Colorimetric/Fluorometric Assay Kit. K265-200 is the same size as the 200 test size of ab102500.

    The kit can perform 200 reactions by fluorometric method or 100 reactions by colorimetric method.

    Hydrogen Peroxide (H2O2) is a reactive oxygen metabolic byproduct that serves as a key regulator for a number of oxidative stress-related states. Functioning through NF-kappaB and other factors, hydroperoxide-mediated pathways have been linked to asthma, inflammatory arthritis, atherosclerosis, diabetic vasculopathy, osteoporosis, neuro-degenerative diseases, Down's syndrome and immune system diseases.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 200 tests
    H2O2 Assay Buffer WM 1 x 25ml
    H2O2 Standard Yellow 1 x 0.1ml
    HRP Green 1 unit
    OxiRed Probe Red 1 x 200µl
  • Research areas

    • Kits/ Lysates/ Other
    • Kits
    • Cell Metabolism Kits
    • Other Metabolism Assay
    • Kits/ Lysates/ Other
    • Kits
    • Cell Damage Kits
    • Oxidative stress
    • Kits/ Lysates/ Other
    • Kits
    • Cell Metabolism Kits
    • Oxidative Stress Assay Kits
    • Oxidative Stress
    • Kits/ Lysates/ Other
    • Kits
    • Cell Damage Kits
    • Antioxidant
  • Relevance

    Hydrogen Peroxide (H2O2) is a reactive oxygen metabolic byproduct that serves as a key regulator for a number of oxidative stress-related states. Functioning through NF-kB and other factors, hydroperoxide-mediated pathways have been linked to asthma, inflammatory arthritis, atherosclerosis, diabetic vasculopathy, osteoporosis, neuro-degenerative diseases, Down’s syndrome and immune system diseases.
  • Alternative names

    • H2O2

Images

  • Functional Studies - Hydrogen Peroxide Assay Kit (ab102500)
    Functional Studies - Hydrogen Peroxide Assay Kit (ab102500)Vicente, Cl?udia S L et al., PloS one?vol. 10,4 e0123839., Fig 2, doi:10.1371/journal.pone.0123839

    H2O2 neutralization of of Bursaphelenchus sp. isolates (high virulence B. xylophilus Ka4 and B. xylophilus T4, and low virulence B. xylophilus C14-5 and B. mucronatus Un1) after 24 hours exposition to H2O2.
    Error bars represent standard deviation. Different letters above the columns indicate significant differences (p < 0.05) between Bursaphelenchus sp. isolates H2O2 neutralization, according to post-hoc Duncan's test.

  • Functional Studies - Hydrogen Peroxide Assay Kit (ab102500)
    Functional Studies - Hydrogen Peroxide Assay Kit (ab102500)
    H2O2 Fluorimetric Standard Curve
  • Functional Studies - Hydrogen Peroxide Assay Kit (ab102500)
    Functional Studies - Hydrogen Peroxide Assay Kit (ab102500)
    H2O2 Colorimetric Standard Curve

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (82)

Publishing research using ab102500? Please let us know so that we can cite the reference in this datasheet.

ab102500 has been referenced in 82 publications.

  • Klopotowska M  et al. PRDX-1 Supports the Survival and Antitumor Activity of Primary and CAR-Modified NK Cells under Oxidative Stress. Cancer Immunol Res 10:228-244 (2022). PubMed: 34853030
  • Che H  et al. Rebalance of the Polyamine Metabolism Suppresses Oxidative Stress and Delays Senescence in Nucleus Pulposus Cells. Oxid Med Cell Longev 2022:8033353 (2022). PubMed: 35178160
  • Younis NS ß-Caryophyllene Ameliorates Cyclophosphamide Induced Cardiac Injury: The Association of TLR4/NF?B and Nrf2/HO1/NQO1 Pathways. J Cardiovasc Dev Dis 9:N/A (2022). PubMed: 35621844
  • Stork BA  et al. Methodology for measuring oxidative capacity of isolated peroxisomes in the Seahorse assay. J Biol Methods 9:e160 (2022). PubMed: 35733440
  • Al Omran AJ  et al. Dihydromyricetin ameliorates social isolation-induced anxiety by modulating mitochondrial function, antioxidant enzymes, and BDNF. Neurobiol Stress 21:100499 (2022). PubMed: 36532369
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-10 of 18 Abreviews or Q&A

Question

Inquiry: I am interested in a kit measuring xanthine oxidase activity in cell lysates (ab102522), as well as a kit for measuring hydrogen peroxide in cell lysates (ab102500). Since there are not many details in the data sheets of the above mentioned kits, I would like to ask the following: 1. Are these kits sensitive for cell lysates and if yes, how many cells per sample should we use to get results? 2. What is the difference between the two kits, since they both use the OxiRed Probe? Thank you in advance for your help.

Read More

Abcam community

Verified customer

Asked on Sep 02 2014

Answer

I can confirm that the kit ab102522 and ab102500 can be used to assay xanthine oxidase and Hydrogen peroxide in cells. I would suggest starting with at least 1-2 million cells. Please follow the following protocol;

2X106 cells, suspend the cell pellet 500 μl (or ˜4 volumes) of the assay buffer on ice, homogenize using a Dounce homogenizer (10-50 passes) on ice, until efficient lysis is confirmed, by viewing the cells under the microscope. Spin down the sample and collect the supernatant. Load the supernatant unto a 10kda spin column for deproteinzation (if indicated in the protcol booklet). Use the eluate for your subsequent assays. Appropriate dilutions of the sample must be tested in order ensure the readings will fall within the linear range of the standard curve.

These kits assay different biochemicals one is specific to XO and other one to H2O2. No doubt the same Oxi red probe is used in the kits as both are based on reaction between H2O2 and oxi red probe; this however doesn’t mean the kits are same.

Read More

Padamjeet Singh

Abcam Scientific Support

Answered on Sep 02 2014

Question

We started to work in our lab with the Hydrogen Peroxide Assay Kit (kat.nomber AB-ab102500).
Can you tell us, based on your data or on publications, what is the
recommended amount to use, per well, from lysate of different rat tissues ?

Read More

Abcam community

Verified customer

Asked on Jan 23 2014

Answer



As recommended on the protocol, an amount ranging from 2 to 50ul should be added to each well, and fill it up to 50ul with assay buffer.

As the concentration of H2O2 in your samples is unknown, you may need to run a quick test beforehand to optimise the volume of sample per well.

As an example, this kit was used on reference PubMed: 24187462, in which 5 μL of the rat sample supernatant was diluted with 46 μL of assay buffer.

Read More

Ariana Veiga

Abcam Scientific Support

Answered on Jan 23 2014

Question

hydrogen peroxide assay kit (ab102500)
is it suitable to assay hydrogen peroxide in cell lysate and in tissue culture supernatante?
is it a must to assay h2o2 after tissue extraction immediately or we can wait a period of time and wht is the max time?

Read More

Abcam community

Verified customer

Asked on Jul 01 2013

Answer

The kit ab102500 is suitable with many type of biological fluids and extracts such as cell lysates and tissue culture supernatant.

Frozen sample can be used with this kit so once the extract is prepared these should be immediately frozen at -20C or -80C for later use. We see people successfully 1-2 year frozen lysates so we unfortunately will not be able to advice any time limit. I would say for better results storage for couple of month should be fine.

Read More

Padamjeet Singh

Abcam Scientific Support

Answered on Jul 01 2013

Question

dear staff, thanks for your answer. last question...how long the signal is stable after 10 minutes incubation time?

Read More

Abcam community

Verified customer

Asked on Mar 15 2013

Answer

Thank you for contacting us.
Since we mention only a 10 min incubation time for this reaction, I would recommend taking the reading right after. Waiting for more time might result in increase in the background signal and later drop in the total signals.
Hope this information has been useful for you. Please let me know if you have any other questions.

Read More

Abcam Scientific Support

Answered on Mar 15 2013

Question

Hi,
Thanks for your email. It is really useful. I would like to order one as soon as possible, so could you provide me the catalog number for this H2O2 assay buffer?

Thank you

Read More

Abcam community

Verified customer

Asked on Mar 06 2013

Answer

Thank you for contacting us.

Please buy the requested product ab167452.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Abcam Scientific Support

Answered on Mar 06 2013

Question

Thanks for your email. It is really useful. I would like to order one as soon as possible, so could you provide me the catalog number for this H2O2 assay buffer?
Thank you

Read More

Abcam community

Verified customer

Asked on Mar 05 2013

Answer

I have sent a request to my colleague for adding this product in catalogue. I will let you know the catalogue number soon.

Thanks!

Read More

Abcam Scientific Support

Answered on Mar 05 2013

Question

We would need H2O2 assay buffer if possible?

Read More

Abcam community

Verified customer

Asked on Mar 04 2013

Answer

Thank you for contacting us.

We can provide one bottle of assay buffer for £90. Please let us know if you are interested in buying.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Abcam Scientific Support

Answered on Mar 04 2013

Question

dear abcam, i work on skeletal muscle tissue. we tested many kits from other company but none were working good.... we have now samples lysed in PBS. we need to test H2O2 quantity in this samples is it possible with you kit? (fluorimetric) have you any experience on skeletal muscle samples? we used the colorimentric one 1 years ago but it was no sensitive enought for us... thanks

Read More

Abcam community

Verified customer

Asked on Mar 04 2013

Answer

Thank you for contacting us.
This kit can actually be used both colorimetrically and fluorometrically. In general fluorometric analysis is ˜10 times more sensitive than colorimetric analysis. The detection limit with this assay can be as low as 2 pmol/well (or 40 nM) of H2O2 in the sensitive fluorometric assay.
As long as the H2O2 concentration is higher than that in the skeletal muscle samples, you would be able to use this kit successfully.
I hope this helps. Please do not hesitate to contact us for further assistance.

Read More

Abcam Scientific Support

Answered on Mar 04 2013

Question

Froze cells at -80C prior to lysis. Following lysis, if 10kDa filter is not used, is it ok to freeze samples? She wants to avoid using the 10kDa filter if possible and will run controls to determine if it causes any variability.

Read More

Abcam community

Verified customer

Asked on Jan 29 2013

Answer

We recommend the use of the 10 kDa filters irrespective of your immediate or later usage of the lysed samples. The filters help in separating out proteins which can chew up the analyte. You most likely will not get efficient results without the fliters.

We carry 10 kD spin columns to assist in using this kit. The link for these columns is given below:
https://www.abcam.com/10kd-spin-column-ab93349.html

Read More

Abcam Scientific Support

Answered on Jan 29 2013

Question

If I am using cells for this kit, can I use any lysis buffer or are there any compatibility issues?

Read More

Abcam community

Verified customer

Asked on Jan 18 2013

Answer

Thank you for contacting us.

You can use the H2O2 assay buffer in the kit as the lysis buffer for cells.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Abcam Scientific Support

Answered on Jan 18 2013

1-10 of 18 Abreviews or Q&A

  •  Previous
  • 1
  • 2
  • Next 

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2023 Abcam plc. All rights reserved.