Isocitrate Dehydrogenase Assay Kit (Colorimetric) (ab102528)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 45 min
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 0.01 mU/well
Overview
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Product name
Isocitrate Dehydrogenase Assay Kit (Colorimetric)
See all Isocitrate Dehydrogenase kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts -
Assay type
Enzyme activity -
Sensitivity
> 0.01 mU/well -
Assay time
0h 45m -
Product overview
Abcam's Isocitrate Dehydrogenase Assay Kit (Colorimetric) provides a convenient tool for sensitive detection of NAD(+) / NADP(+)-dependent or both IDHs in a variety of samples. The IDHs utilize isocitrate as a specific substrate leading to a proportional color development and can be easily quantified colorimetrically (λ = 450 nm) with detection sensitivity as low as 0.01 mU.
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Isocitrate dehydrogenase assay protocol summary:
- add samples and standards to wells
- incubate for 3 min and analyze with microplate reader
- incubate for 30 min - 2 hr and analyze again -
Notes
This product is manufactured by BioVision, an Abcam company and was previously called K756 Isocitrate Dehydrogenase Activity Colorimetric Assay Kit. K756-100 is the same size as the 100 test size of ab102528.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests β-NADP Stock 1 vial Developer Solution II 1 vial IDH Positive Control 1 x 20µl IDH Substrate Mix 1 vial Isocitrate Assay Buffer 1 x 25ml NAD+ 1 vial NADH Standard I 1 vial -
Research areas
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Relevance
Isocitrate dehydrogenase (IDH; EC 1.1.1.41, NAD+) is an enzyme that participates in the citric acid cycle. These IDH3 isoforms catalyze the oxidative decarboxylation of isocitrate, producing alpha-ketoglutarate and CO2 while converting NAD+ to NADH. This is a two-step process, which involves oxidation of isocitrate to oxalosuccinate, followed by the decarboxylation of the beta-carboxyl group to form the ketone, alpha-ketoglutarate. Other isoforms (EC 1.1.1.42, NADP+) catalyze the same reaction, but unrelated to the citric acid cycle. It is carried out in the mitochondrion (IDH2) as well as in the cytosol and peroxisome (IDH1) and use NADP+ as a cofactor instead of NAD+. -
Cellular localization
Cytoplasm. Peroxisome -
Alternative names
- Cytosolic NADP isocitrate dehydrogenase
- EC=1.1.1.42
- IDH
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Images
Datasheets and documents
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SDS download
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Datasheet download
References (17)
ab102528 has been referenced in 17 publications.
- Ming-Chin Lee K et al. Type I interferon antagonism of the JMJD3-IRF4 pathway modulates macrophage activation and polarization. Cell Rep 39:110719 (2022). PubMed: 35443173
- Chocron ES et al. Mitochondrial TrxR2 regulates metabolism and protects from metabolic disease through enhanced TCA and ETC function. Commun Biol 5:467 (2022). PubMed: 35577894
- Vaziri-Gohar A et al. Limited nutrient availability in the tumor microenvironment renders pancreatic tumors sensitive to allosteric IDH1 inhibitors. Nat Cancer 3:852-865 (2022). PubMed: 35681100
- Doulias PT et al. TCA cycle metabolic compromise due to an aberrant S-nitrosoproteome in HIV-associated neurocognitive disorder with methamphetamine use. J Neurovirol 27:367-378 (2021). PubMed: 33876414
- Liu X et al. The existence of a nonclassical TCA cycle in the nucleus that wires the metabolic-epigenetic circuitry. Signal Transduct Target Ther 6:375 (2021). PubMed: 34728602