L-Lactate Assay Kit (Colorimetric/Fluorometric) (ab65330)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 40 min
- Sample type: Cell culture supernatant, Cell Lysate, Other biological fluids, Plasma, Serum, Tissue Lysate, Urine
- Sensitivity: 0.001 mM
Overview
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Product name
L-Lactate Assay Kit (Colorimetric/Fluorometric)
See all L-Lactate kits -
Detection method
Colorimetric/Fluorometric -
Sample type
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Cell Lysate, Tissue Lysate -
Assay type
Quantitative -
Sensitivity
> 0.001 mM -
Range
0.001 mM - 10 mM -
Assay time
0h 40m -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
L-Lactate Assay Kit (Colorimetric/Fluorometric) ab65330 provides a convenient means for detecting L(+)-Lactate in biological samples such as blood, cells, culture mediums, fermentation mediums, etc. There is no need for pretreatment or purification of samples.
In the lactate assay protocol, lactate specifically reacts with an enzyme mix to generate a product, which interacts with a lactate probe to produce color (570 nm) and fluorescence (at Ex/Em = 535/587 nm).
Lactate assay protocol summary:
- add samples (deproteinized) and standards to wells
- add reaction mix and incubate for 30 min at room temp
- analyze with microplate reader -
Notes
This product is manufactured by BioVision, an Abcam company and was previously called K607 Lactate Colorimetric/Fluorometric Assay Kit. K607-100 is the same size as the 100 test size of ab65330.
L(+)-Lactate is the major stereo-isomer of lactate formed in human intermediary metabolism and is present in blood. D(-)-Lactate is also present (see D-Lactate assay kits) but only at about 1-5% of the concentration of L(+)-Lactate.
Alternative L-Lactate assay kits offer different readout modes/wavelengths and sensitivity/range:
- this kit L-Lactate assay ab65330: colorimetric 570 nm, fluorometric Ex/Em 535/587 nm, range 0.001 mM - 10 mM
- our most popular L-Lactate assay ab65331: colorimetric 450nm, range 0.02 mM - 10 mM
- L-Lactate assay ab169557: fluorometric Ex/Em 535/587 nm, range 0.2 µM - 50 µM.Review our Metabolism Assay Guide to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests 2000 tests L(+)-Lactate Standard (100 nmol/µl) Yellow 1 x 100µl 20 x 100µl Lactate Assay Buffer WM 1 x 25ml 20 x 25ml Lactate Enzyme Mix (lyophilized) Green 1 vial 20 vials Lactate Probe (in DMSO, anhydrous) Red 1 x 200µl 20 x 200µl -
Research areas
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Relevance
Lactate (CH3CH(OH)COO-) plays important roles in many biological processes. Abnormal high concentration of lactate has been related to disease states such as diabetes and lactate acidosis, etc. L(+)-Lactate is the major stereoisomer of lactate formed in human intermediary metabolism and is present in blood. The lactate to pyruvate ratio reflects the redox state of the cell and describes the balance beween NAD+ and NADH, which is dependent on the interconversion of lactate and pyruvate via lactate dehydrogenase (LDH).
Images
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Functional studies - ab65330Image from Lai D., PLiS One 8(9), Fig 6C. Doi: 10.1371/journal.pone.0075625. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
HaCaT cells are grown in normoxia for 3 days after infection and medium was tested to determine lactate concentration using L-Lactate assay kit (ab65330). HaCaT cells were infected with AdGFP-18E2 or AdGFP, transfected with Ctrl or HIF-1α siRNA.
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Functional Assay: L-Lactate Assay Kit (Colorimetric/Fluorometric) (ab65330)Relative signal (RFU) in unfiltered human plasma (dilution 1:200) and mouse urine (dilution 1:200), comparing L-lactate signals with background readings ((-); no enzyme) after 10 minutes of incubation (duplicates +/- SD).
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Functional Studies - L-Lactate Assay Kit (ab65330)Standard curve: mean of duplicates (+/- SD) with background reads subtracted
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Functional Studies - L-Lactate Assay Kit (ab65330)Standard curve: mean of duplicates (+/- SD) with background reads subtracted
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Functional Studies - L-Lactate Assay Kit (ab65330)Lactate measured in cell culture supernatants showing quantity (µmol) per mL of tested sample. Samples were diluted 40-160 fold and measured fluorometrically.
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Functional Studies - L-Lactate Assay Kit (ab65330)Lactate measured in biological fluids showing quantity (µmol) per mL of tested sample. Samples were diluted 10-40 fold and measured fluorometrically.
Datasheets and documents
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SDS download
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Datasheet download
References (229)
ab65330 has been referenced in 229 publications.
- Davern M et al. Acidosis significantly alters immune checkpoint expression profiles of T cells from oesophageal adenocarcinoma patients. Cancer Immunol Immunother 72:55-71 (2023). PubMed: 35708739
- Wang CS et al. NBR2/miR-561-5p/DLC1 axis inhibited the development of multiple myeloma by activating the AMPK/mTOR pathway to repress glycolysis. Neoplasma 69:1165-1174 (2022). PubMed: 35951458
- Ding D et al. Non-canonical function of an Hif-1a splice variant contributes to the sustained flight of locusts. Elife 11:N/A (2022). PubMed: 36039636
- Shyam R et al. Rescue of the Congenital Hereditary Endothelial Dystrophy Mouse Model by Adeno-Associated Viruse-Mediated Slc4a11 Replacement. Ophthalmol Sci 2:N/A (2022). PubMed: 36051248
- Chen J et al. Activated Hepatic Stellate Cells Promote the M1 to M2 Macrophage Transformation and Liver Fibrosis by Elevating the Histone Acetylation Level. Dis Markers 2022:9883831 (2022). PubMed: 36133436