Product nameLysosomal Staining Kit - Blue Fluorescence - Cytopainter
See all Lysosomes kits
Sample typeAdherent cells, Suspension cells
Lysosomal Staining Kit - Blue Fluorescence | Cytopainter (ab112135) is designed to label lysosomes in live cells in blue fluorescence at Ex/Em = 350/440nm. Ab112135 uses a proprietary lysotropic dye that selectively accumulates in lysosomes probably via the lysosome pH gradient. The lysotropic indicator is a hydrophobic compound that easily permeates intact live cells, and is trapped in lysosomes after it gets into the cells. Its fluorescence is significantly enhanced upon entering lysosomes. This key feature significantly reduces its staining background. The labeling protocol is robust, requiring minimal hands-on time.
Ab112135 can be readily adapted for many different types of fluorescence platforms such as microplate assays, flow cytometry and fluorescence microscope. It is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. Ab112135 provides all the essential components with an optimized cell-labeling protocol and can be used for both proliferating and non-proliferating cells (either suspension or adherent cells).
Abcam's fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as lysosomes, mitochondria,and actin filaments. The selective staining of cell compartments provides a powerful method for studying cellular events in a spatial and temporal context.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 500 tests Live Cell Staining Buffer 1 x 50ml LysoBlue Indicator 1 x 100µl
- lysosome marker
ab112135 has been referenced in 4 publications.
- LeBlond ND et al. Foam Cell Induction Activates AMPK But Uncouples Its Regulation of Autophagy and Lysosomal Homeostasis. Int J Mol Sci 21:N/A (2020). PubMed: 33261140
- Purdie L et al. Alkyl-Modified Oligonucleotides as Intercalating Vehicles for Doxorubicin Uptake via Albumin Binding. Mol Pharm 15:437-446 (2018). PubMed: 29265823
- Liu F et al. Parallel comparative studies on toxicity of quantum dots synthesized and surface engineered with different methods in vitro and in vivo. Int J Nanomedicine 12:5135-5148 (2017). PubMed: 28790821
- Purdie L et al. Influence of Polymer Size on Uptake and Cytotoxicity of Doxorubicin-Loaded DNA-PEG Conjugates. Bioconjug Chem 27:1244-52 (2016). PubMed: 27074730