Lysosomal Staining Kit - Green Fluorescence - Cytopainter (ab112136)
Overview
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Product name
Lysosomal Staining Kit - Green Fluorescence - Cytopainter
See all Lysosomes kits -
Sample type
Adherent cells, Suspension cells -
Assay type
Cell-based -
Product overview
Abcam's fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as lysosomes, mitochondria, and actin filaments. The selective staining of cell compartments provides a powerful method for studying cellular events in a spatial and temporal context.
ab112136 is designed to label lysosomes in live cells with green fluorescence at Ex/Em = 490/525 nm. The proprietary lysotropic dye used in the kit selectively accumulates in lysosomes probably via the lysosome pH gradient. The lysotropic indicator is a hydrophobic compound that easily permeates intact live cells, and is trapped in lysosomes after it gets into cells. Its fluorescence is significantly enhanced upon entering lysosomes. This key feature significantly reduces its staining background and makes it useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. It is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells.
Review other dyes and kits for lysosomal staining, or the live cell staining fluorescent dyes guide
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Platform
Fluorescence microscope
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 500 tests Live Cell Staining Buffer 1 x 50ml LysoGreen Indicator 1 x 100µl -
Research areas
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Alternative names
- lysosome marker
- lysotracker
Images
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Fluorescence Microscopy - Lysosomal Staining Kit - Green Fluorescence (ab112136)
ab112136 staining U2OS cells by fluorescence microscopy (in a black 96 well plate).
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab112136 has been referenced in 3 publications.
- Sultana F et al. Snx10 and PIKfyve are required for lysosome formation in osteoclasts. J Cell Biochem 121:2927-2937 (2020). PubMed: 31692073
- Liu F et al. Parallel comparative studies on toxicity of quantum dots synthesized and surface engineered with different methods in vitro and in vivo. Int J Nanomedicine 12:5135-5148 (2017). PubMed: 28790821
- Aasen SN et al. A Novel Nanoprobe for Multimodal Imaging Is Effectively Incorporated into Human Melanoma Metastatic Cell Lines. Int J Mol Sci 16:21658-80 (2015). PubMed: 26370983