Lysosomal Staining Kit - Red Fluorescence - Cytopainter (ab112137)
Overview
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Product name
Lysosomal Staining Kit - Red Fluorescence - Cytopainter
See all Lysosomes kits -
Sample type
Adherent cells, Suspension cells -
Assay type
Cell-based -
Product overview
Abcam's fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as lysosomes, mitochondria, and actin filaments in live cells. The selective staining of cell compartments provides a powerful method for studying cellular events in a spatial and temporal context.
ab112137 is designed to label lysosomes in live cells with red fluorescence at Ex/Em = 575/600 nm. The kit uses a proprietary lysotropic dye that selectively accumulates in lysosomes probably via the lysosome pH gradient. The lysotropic indicator is a hydrophobic compound that easily permeates intact live cells, and becomes trapped inside lysosomes after it gets into cells. Its fluorescence is strongly enhanced upon entering lysosomes. This key feature significantly reduces its staining background. The labeling protocol is robust, requiring minimal hands-on time. ab112137 can be readily adapted for many types of fluorescence platforms such as microplate assays, flow cytometry and fluorescence microscope. It is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. The kit provides all the essential components and it can be used for both proliferating and non-proliferating cells (either suspension or adherent cells).
Review other dyes and kits for lysosomal staining, or the live cell staining fluorescent dyes guide
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Platform
Fluorescence microscope
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 500 tests Live Cell Staining Buffer 1 x 50ml LysoRed Indicator 1 x 100µl -
Research areas
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Alternative names
- lysosome marker
- lysotracker
Images
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Fluorescence Microscopy - Lysosomal Staining Kit - Red Fluorescence (ab112137)
ab112137 staining U2OS cells by Fluorescence microscopy (in a black 96 well plate). Incubation time is 20 min to 1 hour and exposure time 20 ms to 100 ms.
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab112137 has been referenced in 4 publications.
- Linden JR et al. Clostridium perfringens epsilon toxin induces blood brain barrier permeability via caveolae-dependent transcytosis and requires expression of MAL. PLoS Pathog 15:e1008014 (2019). PubMed: 31703116
- Miao JK et al. Lysosomal dysfunction disturbs porcine oocyte maturation and developmental capacity by disorganizing chromosome/cytoskeleton and activating autophagy/apoptosis. Theriogenology 140:44-51 (2019). PubMed: 31437668
- Redko B et al. Toward the development of a novel non-RGD cyclic peptide drug conjugate for treatment of human metastatic melanoma. Oncotarget 8:757-768 (2017). IF ; Human . PubMed: 27768593
- Luo W et al. Subcellular location and photodynamic therapeutic effect of chlorin e6 in the human tongue squamous cell cancer Tca8113 cell line. Oncol Lett 9:551-556 (2015). PubMed: 25621023