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Magnesium Assay Kit (ab102506)

Submit a review Q&A (6)References (4)
Functional Studies - Magnesium Detection Kit (ab102506)
  • Functional Studies - Magnesium Detection Kit (ab102506)
  • Functional Studies - Magnesium Detection Kit (ab102506)
  • Functional Studies - Magnesium Detection Kit (ab102506)

Key features and details

  • Assay type: Quantitative
  • Platform: Microplate reader
  • Assay time: 30 min
  • Sample type: Cell culture media, Cell Lysate, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
  • Sensitivity: 40 µM

Overview

  • Product name

    Magnesium Assay Kit
    See all Magnesium kits

  • Sample type

    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media

  • Assay type

    Quantitative

  • Sensitivity

    40 µM

  • Range

    2 µM - 15 µM

  • Assay time

    0h 30m

  • Product overview

    Magnesium Assay Kit (ab102506) is a simple sensitive assay for quantitating magnesium in a variety of biological samples. The kit takes advantage of the specific requirement of glycerol kinase for Mg2+. An enzyme linked reaction leads to formation of an intensely colored (λmax = 450nm) product whose formation is proportional to Mg2+ concentration. The linear range of the assay is 2-15 nmoles with detection sensitivity ~40 µM.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    This product is manufactured by BioVision, an Abcam company and was previously called K385 Magnesium Colorimetric Assay Kit. K385-100 is the same size as the 100 test size of ab102506.

    Magnesium is the 11th most abundant element by mass in the human body. Mg2+ is essential to all living cells where it plays an important role in facilitating the processing of biological polyphosphates like ATP, DNA, RNA and enzyme functions. Mg2+ is the metallic ion at the center of chlorophyll, and a common additive to fertilizers. Mg2+ compounds are used as laxatives, antacids, and used to stabilize abnormal nerve excitation and blood vessel spasm i.e., eclampsia.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests 2000 tests
    Magnesium Assay Buffer WM 1 x 25ml 20 x 25ml
    Magnesium Developer Purple 1 unit 20 units
    Magnesium Enzyme Mix Green 1 unit 20 units
    Magnesium Standard Yellow 1 x 0.1ml 20 x 0.1ml

  • Research areas

  • Relevance

    Magnesium is the 11th most abundant element by mass in the human body. Magnesium is essential to all living cells where it plays an important role in facilitating the processing of biological polyphosphates like ATP, DNA, RNA and enzyme functions. Magnesium is the metallic ion at the center of chlorophyll, and a common additive to fertilizers. Magnesium compounds are used as laxatives, antacids, and used to stabilize abnormal nerve excitation and blood vessel spasm i.e., eclampsia.

  • Alternative names

    • Mg++
    • Mg+2
    • Mg2+

Images

  • Functional Studies - Magnesium Detection Kit (ab102506)
    Functional Studies - Magnesium Detection Kit (ab102506)

    Magnesium measured in mouse tissue lysates showing quantity (nmol) per mg protein of tested sample

  • Functional Studies - Magnesium Detection Kit (ab102506)
    Functional Studies - Magnesium Detection Kit (ab102506)

    Magnesium measured in biological fluids showing quantity (nmol) per microliter of tested sample

  • Functional Studies - Magnesium Detection Kit (ab102506)
    Functional Studies - Magnesium Detection Kit (ab102506)

    Colourimetric standard curve : mean of duplicates (+/-SD) with background reads substracted

  • Functional Studies - Magnesium Detection Kit (ab102506)
    Functional Studies - Magnesium Detection Kit (ab102506)
    Magnesium standard curve: Assay is performed according to kit protocol. Vertical dotted lines indicate the lower and upper limits of normal serum Magnesium concentrations.

References (4)

Publishing research using ab102506? Please let us know so that we can cite the reference in this datasheet.

ab102506 has been referenced in 4 publications.

  • Kwon JG  et al. Mouse feeding study and microbiome analysis of sourdough bread for evaluation of its health effects. Front Microbiol 13:989421 (2022). PubMed: 36212840
  • Roller BL  et al. Elution properties of a resorbable magnesium phosphate cement. J Clin Orthop Trauma 11:S729-S734 (2020). PubMed: 32999547
  • Lanaspa MA  et al. Ketohexokinase C blockade ameliorates fructose-induced metabolic dysfunction in fructose-sensitive mice. J Clin Invest 128:2226-2238 (2018). PubMed: 29533924
  • Zhang LW & Warrington JP Magnesium Sulfate Prevents Placental Ischemia-Induced Increases in Brain Water Content and Cerebrospinal Fluid Cytokines in Pregnant Rats. Front Neurosci 10:561 (2016). Rat . PubMed: 28008305

Customer reviews and Q&As

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Submit a review Submit a question

1-6 of 6 Abreviews or Q&A

Question

I'm interested in the Calcium Colorimetric Assay.

I have a few technical questions about the assay and the materials.

According to the website the linear range of detection for this kit is between 0.1-25 mM? What about higher that?

Will other ions interfere with the measurements? Meaning presence of other divalent or monovalent ions?

In my solution there are also presence of MOPS ions and NaCl.  

What is the binding molecule that binds to the Ca ions? In Sigma it is the o-cresolphthalein molecule. Is the binding of your molecule overcome other interaction with other ions? Meaning that the binding is specific and the presence of other ions do not interfere with the measurement.

I'm also looking to measure the concertation of Mg and Zn. I found Magnesium Assay Kit, does it works with different binding molecule? Do they interfere with one another. Is it specific as well? What is the binding molecule in the Mg kit?

Is it possible to buy the binding molecule and not the entire kit?

 

 




 



 


Keren Haddad, M.Sc.,


Product Manager


Zotal Ltd


http://mail.zotal.co.il/exchweb/bin/redir.asp?URL=http://mail.zotal.co.il/exchweb/bin/redir.asp?URL=http://mail.zotal.co.il/exchweb/bin/redir.asp?URL=http://mail.zotal.co.il/exchweb/bin/redir.asp?URL=http://mail.zotal.co.il/exchweb/bin/redir.asp?URL=http://mail.zotal.co.il/exchweb/bin/redir.asp?URL=http://www.zotal.co.il


Tel: +972 (0) 36492444


Cell: +972 (0) 544330802


Fax: +972 (0)3 6496664

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Answer

Thank you for getting in touch. I am happy to provide answers to your questions:

1. According to the website the linear range of detection for this kit is between 0.1-25 mM? What about higher that?

It is possible to detect higher amount as well- however, we have not tested it and it may be above the accurate range of the assay.

2. Will other ions interfere with the measurements? Meaning presence of other divalent or monovalent ions?

ab102506 Magnesium Detection Kit did not give interference from other ions tested- Zn, Mn, Ni, Fe, Cu, Co, Ca

ab102505 Calcium Detection Kit (Colorimetric) not known to show interference from Fe+2 or Mg

3. In my solution there are also presence of MOPS ions and NaCl.  

In testing, for both kits ab102505 and ab102506 we didn’t see an issue with NaCl , however, MOPS was not tested.

4. What is the binding molecule that binds to the Ca ions? In Sigma it is the o-cresolphthalein molecule. Is the binding of your molecule overcome other interaction with other ions? Meaning that the binding is specific and the presence of other ions do not interfere with the measurement.

For the calcium assay ab102505 we have a proprietary buffer formulation that prevents interference from Fe and Mg.

5. I'm also looking to measure the concertation of Mg and Zn. I found Magnesium Assay Kit, does it works with different binding molecule? Do they interfere with one another. Is it specific as well?

6. What is the binding molecule in the Mg kit?

There are no binding molecules in the magnesium assay kit ab102506  - the assay is based on a reaction where Mg serves as a co-factor.


7. Is it possible to buy the binding molecule and not the entire kit?


Unfortunately, I am sorry this is not possible in this case.


I am happy to answer any further questions you have.

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Question

I tried another standard and set of reagents today and the assay worked fine! Thank you for your help trying to resolve this.

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Answer

I am happy to hear that the kit is working for you! Please do not hesitate to contact me if I can be of any further assistance.

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Question

The enzyme mixture was reconstituted in 550 uL of assay buffer. It was stored at -20 C.

The standards were prepared by mixing 10 uL of magnesium standard with 990 uL of water. The remaining standards were diluted down from that stock solution.

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Answer

Thank you for your reply.


We have rechecked the QC and retested the kit which is working as expected, therefore it is unclear why the kit is not working well in your hands. As this kit is covered by the Abpromise I am happy to offer a replacement or credit. Please let me know which you would prefer.

I look forward to your reply so that I may assist you further. Please do not hesitate to contact us if you have any additional questions or concerns.

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Question

The kit was reconstituted 17-FEB-2012. I believe it was received a few days before that. The curve was repeated the following day with similar results. Once the enzyme mixture was added to the samples, all of the standards turned color at the same relative intensity. The kit/reagents have been stored at -20 C. Thanks for your help.

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Answer

Thank you for your reply.


The lab has some additional questions: How was theenzyme reconstituted/stored? Can you also describe how standards were prepared?


We want to be sure we cover all possibilities since the QC looks great for this lot. I look forward to your reply so that I may assist you further.

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Question

Thank you for your help. Here is the excel file for the data we generated. This was a standard curve. 1.1 mL of water was used to reconstitute the developer and 550 uL was used to reconstitute the enzyme. The rest of the protocol was followed. We repeated the curve on a second day and got similar results. Let me know if you have any ideas, please. Thanks.

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Answer

Thank you for your patience as I have investigated this problem further.


Based on the data you show it looks like the issue is thatno reaction mix or old/expired mix was used as there is not even the hint of a difference between 0 and the rest of the standards.


How old is this kit? When was it first reconstituted? Storage temp after? Have you tried to repeat the assay fresh (not the replicates shown)?


Also, can you please provide a lot number and order number for this kit?


I look forward to your reply so that I may assist you further.

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Question

I've followed the protocol, but I'm seeing a "flat" standard curve.

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Answer

I am sorry to hear that this kit is not providing satisfactory results.

Could you please send me the data in an Excel format so we may take a look and make any suggestions as necessary? The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality.

In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund.

I hope this information is helpful, and I thank you for your cooperation.

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