Mitochondrial Staining Kit - Red Fluorescence - Cytopainter (ab112145)
Overview
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Product name
Mitochondrial Staining Kit - Red Fluorescence - Cytopainter
See all Mitochondria kits -
Sample type
Adherent cells, Suspension cells -
Assay type
Cell-based -
Product overview
Abcam's fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as lysosomes, mitochondria, and actin filaments. The selective staining of cell compartments provides a powerful method for studying cellular events in a spatial and temporal context.
ab112145 is designed to label mitochondria in live cells with red fluorescence. The kit uses our proprietary dye that selectively accumulates in mitochondria probably via the mitochondrial membrane potential gradient. The red fluorescent mitochondrial stain used in the kit has Ex/Em = 580/600 nm. The mitochondrial indicator, a hydrophobic compound, easily permeates intact live cells and becomes trapped in mitochondria after it gets into cells. This fluorescent mitochondrial indicator is retained in mitochondria for a long time since it carries a cell-retaining group. This key feature significantly increases the staining efficiency.
ab112145 can be readily adapted for many different types of fluorescence platforms, such as microplate assays, immunocytochemistry and flow cytometry. It is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. The kit provides all the essential components and can be used for both proliferating and non-proliferating cells.
Review other dyes and kits for mitochondrial staining, or the live cell staining fluorescent dyes guide
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Notes
Mitochondria are membrane-enclosed organelles found in most eukaryotic cells. Mitochondria are sometimes described as “cellular power plants” because they generate most of the cellular supply of ATP. In addition to supplying cellular energy, mitochondria are involved in a range of other processes, such as signaling, cellular differentiation, cell death, as well as the control of the cell cycle and cell growth. Mitochondria have been implicated in several human diseases, including mitochondrial disorders and cardiac dysfunction, and may play a role in the aging process. Although most of a cellular DNA is contained in the cell nucleus, the mitochondrion has its own independent genome.
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Platform
Fluorescence microscope
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 500 tests Live Cell Staining Buffer 1 x 50ml MitoRed Indicator 1 x 100µl -
Research areas
Images
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Fluorescence Microscopy- CytoPainter Mitochondrial Staining Kit - Red Fluorescence (ab112145)Image of U2OS cells stained with ab112145 in a black 96-well plate.
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab112145 has been referenced in 8 publications.
- Li S et al. Sleeve Gastrectomy-Induced AMPK Activation Attenuates Diabetic Cardiomyopathy by Maintaining Mitochondrial Homeostasis via NR4A1 Suppression in Rats. Front Physiol 13:837798 (2022). PubMed: 35360240
- Fan L et al. N-Acetylcysteine improves oocyte quality through modulating the Nrf2 signaling pathway to ameliorate oxidative stress caused by repeated controlled ovarian hyperstimulation. Reprod Fertil Dev 34:736-750 (2022). PubMed: 35513370
- Liang S et al. Accumulated ROS Activates HIF-1a-Induced Glycolysis and Exerts a Protective Effect on Sensory Hair Cells Against Noise-Induced Damage. Front Mol Biosci 8:806650 (2021). PubMed: 35096971
- Lee DH et al. Sesamol Increases Ucp1 Expression in White Adipose Tissues and Stimulates Energy Expenditure in High-Fat Diet-Fed Obese Mice. Nutrients 12:N/A (2020). PubMed: 32443555
- Chen T et al. ROS-Mediated Mitochondrial Dysfunction and ER Stress Contribute to Compression-Induced Neuronal Injury. Neuroscience 416:268-280 (2019). PubMed: 31425734
- Peng S et al. Achyranthes bidentata polypeptide protects dopaminergic neurons from apoptosis induced by rotenone and 6-hydroxydopamine. Neural Regen Res 13:1981-1987 (2018). PubMed: 30233073
- Choi JH et al. Oxyresveratrol Increases Energy Expenditure through Foxo3a-Mediated Ucp1 Induction in High-Fat-Diet-Induced Obese Mice. Int J Mol Sci 20:N/A (2018). PubMed: 30577593
- Song NJ et al. Prdm4 induction by the small molecule butein promotes white adipose tissue browning. Nat Chem Biol 12:479-81 (2016). PubMed: 27159578