Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 30 min
- Sample type: Cell culture supernatant, Tissue Extracts
- Sensitivity: 0.0005 mU/well
Product nameMyeloperoxidase (MPO) Activity Assay Kit (Fluorometric)
See all Myeloperoxidase kits
Sample typeCell culture supernatant, Tissue Extracts
Assay typeEnzyme activity
Sensitivity> 0.0005 mU/well
Assay time0h 30m
Species reactivityReacts with: Mammals, Other species
Myeloperoxidase (MPO) Activity Assay Kit ab111749 provides a rapid, simple and reliable fluorometric assay to study MPO activity.
In the MPO assay protocol, MPO catalyzes the production of sodium hypochlorite (NaClO) from hydrogen peroxide (H2O2) and sodium chloride (NaCl). Subsequently, NaClO reacts stoichiometrically with the free radical sensor Aminophenyl fluorescein (APF) to generate fluorescein, whose fluorescence can be detected at Ex/Em = 485/525 nm.
This MPO assay kit can be used to detect MPO activity as low as 0.5 µU per well.
MPO assay protocol summary:
- add fluoroscein standard to wells and analyze with a microplate reader
- add samples to wells
- add reaction mix
- analyze every 2-3 mins with a microplate reader in kinetic mode for at least 30 mins
This product is manufactured by BioVision, an Abcam company and was previously called K745 Myeloperoxidase (MPO) Fluorometric Activity Assay Kit. K745-100 is the same size as the 100 test size of ab111749.
Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 100 tests Assay Buffer WM 1 x 25ml Fluorescein Standard (1 mM) Yellow cap 1 x 50µl MPO Positive Control Purple cap 1 vial MPO Probe Red cap 1 x 200µl MPO Substrate Stock Blue cap 1 x 50µl
FunctionPart of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.
Involvement in diseaseDefects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis.
Sequence similaritiesBelongs to the peroxidase family. XPO subfamily.
- Information by UniProt
- 84 kDa myeloperoxidase
- 89 kDa myeloperoxidase
- EC 22.214.171.124
ab111749 has been referenced in 16 publications.
- Hunt BC et al. Nontypeable Haemophilus influenzae Redox Recycling of Protein Thiols Promotes Resistance to Oxidative Killing and Bacterial Survival in Biofilms in a Smoke-Related Infection Model. mSphere 7:e0084721 (2022). PubMed: 35044805
- Wan H et al. Capsaicin inhibits intestinal Cl- secretion and promotes Na+ absorption by blocking TRPV4 channels in healthy and colitic mice. J Biol Chem 298:101847 (2022). PubMed: 35314195
- Hou W et al. Gu-Ben-Zhi-Ke-Zhong-Yao Alleviated PM2.5-Induced Lung Injury via HMGB1/NF-?B Axis. J Healthc Eng 2022:8450673 (2022). PubMed: 35399858
- Li L et al. TNFAIP6 defines the MSC subpopulation with enhanced immune suppression activities. Stem Cell Res Ther 13:479 (2022). PubMed: 36153571
- Nie Y et al. PolyADP-Ribosylation of NFATc3 and NF-?B Transcription Factors Modulate Macrophage Inflammatory Gene Expression in LPS-Induced Acute Lung Injury. J Innate Immun 13:83-93 (2021). PubMed: 33045713