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PDH Immunocapture Kit (ab109802)

Submit a review Q&A (6)References (2)
Immunoprecipitation - PDH Immunocapture Kit (ab109802)

    Key features and details

    • Assay type: Quantitative

    Overview

    • Product name

      PDH Immunocapture Kit
      See all Pyruvate dehydrogenase kits

    • Assay type

      Quantitative

    • Species reactivity

      Reacts with: Cow, Human

    • Product overview

      250 µg monoclonal antibody irreversibly crosslinked to protein G-agarose beads which can immunocapture PDH complex from heart mitochondria.


      Note: The immunocapture protocol for this kit requires Abcam reagent detergent lauryl maltoside (ab109857/MS910).


       

    Associated products

    Images

    • Immunoprecipitation - PDH Immunocapture Kit (ab109802)
      Immunoprecipitation - PDH Immunocapture Kit (ab109802)

      PDH immunoprecipitation using antibody ab109866/MSP01c crosslinked to protein G-agarose beads as product ab109802

    References (2)

    Publishing research using ab109802? Please let us know so that we can cite the reference in this datasheet.

    ab109802 has been referenced in 2 publications.

    • Stenson BM  et al. Activation of liver X receptor regulates substrate oxidation in white adipocytes. Endocrinology 150:4104-13 (2009). PubMed: 19556420
    • Bonnet S  et al. A mitochondria-K+ channel axis is suppressed in cancer and its normalization promotes apoptosis and inhibits cancer growth. Cancer Cell 11:37-51 (2007). PubMed: 17222789

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-6 of 6 Abreviews or Q&A

    Question

    How many tests can be performed with each size kit?  Can you please send me a quote (in GBP) for this kit and for an antibody to detect the E1 alpha subunit?

    Read More
    Answer

    Thank you for contacting us. 
    This kit size refers to the amount of antibody.  For example, the 250 ug kit contains 250 ug of antibody conjugated to 25 uL of beads (settled volume) suspended in a total volume of 400 uL. Thus the concentration of the antibody is 10 ug per uL of beads, or 0.625 ug per uL of whole suspension.  The 250 ug, 500 ug, and 750 ug sizes can capture ˜13 ug, ˜26 ug, or ˜39 ug respectively of PDH complex from heart mitochondria. 
    For detecting Pyruvate Dehydrogenase E1-alpha, I would recommend either ab110330 or ab110334.  I have included all three sizes of the kit and both antibodies on the quote I sent you for your reference.  If you could let me know your shipping address and the products you are interested in, I will be happy to update the quote for you.

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    Question

    Inquiry: Hi, I am interested in trying this product with rat samples. Do you have any direct evidence for its efficacy against rat PDH, do you know if it was raised against a sequence that is identical to rat? The product information states that it reacts with human and cow, and I am concerned that there are no "predicted" interactions despite the high sequence identity of PDH subunits between human, mouse and rat, etc.

    Read More
    Answer

    Thank you for contacting us.

    I have had the opportunity to speak with the lab regarding this on your behalf. They recommend using ab110333 (MSP06) with rat samples. The antibody targets on E2/E3 binding protein but pulls down the whole PDH complex using IP reagents and protocol. The product page for ab110333 is located at:

    https://www.abcam.com/Pyruvate-dehydrogenase-E2-E3bp-antibody-13G2AE2BH5-ab110333.html

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Question

    Hello,
    I have been already helped because I sent her a separate email amended to my previous inquiry from half a year ago. I also got another reference number yesterday from mailto:uk.technical@abcam.com which is different from the one you just gave me. I'm sorry to be causing so much confusion. All I wanted to say is that all three of these reference numbers can be closed now because my inquiry has already been taken care of.
    Thank you very much and have a nice day!
    Sincerely,

    Read More
    Answer

    Thank you for your previous email and I apologize that I'm just now getting around to reading it! I'll close any other correspondance so you won't be bothered again about this enquiry.

    If you need any further information about these products please let me know and I'll be happy to help! Have a lovely day.

    Read More

    Question

    Hello again,
    I had contacted you with a question concerning your PDH-capture antibody (ab109802 or MSP01) about half a year ago, and got a very good answer, so I was wondering if I could ask one more thing. I realize that it most likely is not possible to disclose the immunogen used in generation of this antibody but I was wondering if it's been verified that the antibody captures only the pyruvate dehydrogenase complex or whether there's a possibility of cross-reactivity with the branched-chain-alpha-ketoacid dehydrogenase, since they are very similar.
    Thank you very much in advance!

    Read More
    Answer

    Thank you for contacting us with your question, and for your patience while I have been in touch with the lab about the specificity of this antibody.
    My contact at the lab has responded:
    "We did mass spectrometry and immunoblotting using this antibody and did not see any other branched chain dehydrogenases represented such as OGDH subunits.Enzymatically they are quite similar, but the primary sequences are not that similar. E.g. PDH E2 – OGDH E2 are only 29% identical."
    I hope this information will be useful, but please let me know if you have any further questions and I'll be happy to help you. Have a great day!

    Read More

    Question

    Hello, I'm interested in your PDH capture antibody (MSP01) but I was wondering if you could tell me, whether the antibody captures the whole complex along with the kinases and phosphatases. So not just E1a, E1b, E2, E3, E3BP but also the PDKs (PDK1,2,3,4) and PDPs (PDP1,2)? Thank you for your help in advance!

    Read More
    Answer

    Thank you for contacting us and for your interest in MSP01 (ab109802). I forwarded your email to the lab scientists at MitoSciences, and I received the following response: We do not know if the kinase and phosphatase are present, but if so then they are at low levels because we did not pick them up by mass spectrometry. We do know the enzyme is active.  Addition of ATP inhibits the enzyme activity which might suggest the presence of PDH kinases, however we do not see a corresponding increase in phosphorylation of PDH E1 alpha subunit. My thoughts are that they may be there in small amounts. I hope this information is helpful, but please let me know if you have any further questions.

    Read More

    Question

    I have a customer interested in the following product:

    # MSP01-500 (ab109802) Pyruvate Dehydrogenase Complex (PDH) Immunocapture kit

    Can you give evidence to prove this immunocapture works. She is planning to apply this kit in mouse tissue.

    Thank you in advance!

    Best regards,

    Read More
    Answer

    Thank you for contacting us.

    We haven't tested this kit with mouse samples so we unfortunately cannot provide any details. The kit has been optimized with cow and human samples so these samples are fully guaranteed.

    We however recommend using ab110333 (MSP06) with mouse samples. The antibody targets on E2/E3 binding protein but pulls down the whole PDH complex using IP reagents and protocol. The product page for ab110333 is located at:

    https://www.abcam.com/Pyruvate-dehydrogenase-E2-E3bp-antibody-13G2AE2BH5-ab110333.html

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

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