Key features and details
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Other biological fluids, Plasma, Serum
Product namePepsin/Pepsinogen Assay Kit (Fluorometric)
See all Pepsin kits
Sample typeSerum, Plasma, Other biological fluids
Pepsin/Pepsinogen Assay Kit (Fluorometric) (ab238722) is a homogenous assay that allows for quantification of pepsin activity in gastric tissues (stomach, duodenum etc.) and various biological fluids (serum/plasma, gastric juice, vomit). The assay utilizes a synthetic peptide substrate bearing both a fluorophore and a fluorescence quencher. Upon cleavage by pepsin, the fluorophore-bearing peptide fragment is unquenched to produce a bright fluorescent signal (Ex/Em = 328/418 nm). Lysosomal aspartic proteases in the peptidase A1 family (Cathepsin D and E) do not interfere with the assay. The assay is rapid, simple to perform and is vastly more sensitive than the classical hemoglobin degradation assay, with a detection limit of 500 μU pepsin activity per well.
This product is manufactured by BioVision, an Abcam company and was previously called K446 Pepsin/Pepsinogen Activity Assay Kit (Fluorometric). K446-100 is the same size as the 100 test size of ab239722.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 100 tests Mca Standard (1mM) 1 x 25µl Pepsin Assay Buffer 1 x 25ml Pepsin Inhibitor (Pepstatin A) 1 x 20µl Pepsin Positive Control 1 vial Pepsin Substrate 1 x 200µl
RelevancePepsin is a digestive enzyme with particularly broad specificity. It is stored as pepsinogen, so only released when needed. In the stomach, chief cells release pepsinogen. The hormone gastrin and the vagus nerve trigger the release of both pepsinogen and HCl from the stomach lining when food is ingested. HCl creates an acidic environment which allows pepsinogen to unfold and cleave itself in an autocatalytic fashion, thereby generating pepsin (the active form). Pepsin may then cleave the dietary proteins into smaller peptides.
- Pepsin A
- Pepsin A 3
ab239722 has not yet been referenced specifically in any publications.