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Phosphatidylglycerol Assay Kit (Fluorometric) (ab273288)

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PG standard curve.
  • Determination of total PG concentration in lipid extracted from brain lysate.
  • Determination of Phosphatidylglycerol in lipid extracted from lung tissue.
  • Specificity of assay.

Key features and details

  • Assay type: Quantitative
  • Detection method: Fluorescent
  • Platform: Microplate
  • Sample type: Cell culture extracts, Tissue Extracts

Overview

  • Product name

    Phosphatidylglycerol Assay Kit (Fluorometric)

  • Detection method

    Fluorescent

  • Sample type

    Cell culture extracts, Tissue Extracts

  • Assay type

    Quantitative

  • Assay duration

    Multiple steps standard assay

  • Product overview

    Phosphatidylglycerol Assay Kit (Fluorometric) (ab273288) utilizes specific enzymes to generate an intermediate that reacts with a probe. This yields a signal that can be quantified fluorometrically and is proportional to the amount of Phosphatidylglycerol (PG) present in the sample, unaffected by the presence of phosphatidic acid, cardiolipin, or other lipids. When used as described, the assay is highly selective against other phospholipids and capable of detecting as little as 20 pmoles of phosphatidylglycerol.

  • Notes

    This product is manufactured by BioVision, an Abcam company and was previously called K488 Phosphatidylglycerol Assay Kit (Fluorometric). K488-100 is the same size as the 100 test size of ab273288.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    PG Assay Buffer 1 x 25ml
    PG Converter Mix (Lyophilized) 1 vial
    PG Enzyme Mix (Lyophilized) 1 vial
    PG Probe (in DMSO) 1 x 200µl
    PG Standard (0.5 µM) (Lyophilized) 1 vial

Images

  • PG standard curve.
    PG standard curve.

    Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

  • Determination of total PG concentration in lipid extracted from brain lysate.
    Determination of total PG concentration in lipid extracted from brain lysate.
  • Determination of Phosphatidylglycerol in lipid extracted from lung tissue.
    Determination of Phosphatidylglycerol in lipid extracted from lung tissue.
  • Specificity of assay.
    Specificity of assay.

    Specificity of assay, when used as described, for Phosphatidylglycerol (PG) versus Phosphatidylethanolamine (PE), Phosphatidic Acid (PA), Phosphatidylinositol (PI), and cardiolipin (CL). The amount of phospholipid for each sample was 1 nmole.

References (0)

Publishing research using ab273288? Please let us know so that we can cite the reference in this datasheet.

ab273288 has not yet been referenced specifically in any publications.

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