Pyruvate Assay Kit (ab65342)

Thank you for your enquiry regarding ab65342.

I apologize for my delayed response but was out of the office yesterday.

You can homogenize about 2 x 10^6 cells with 500 µl of the assay buffer, centrifuge it and take the supernatant for this assay. We would suggest using several dilutions/concentrations of your sample to ensure the readings are within the standard curve range. Due to the presence of LDH in serum, care must be taken during sample processing to prevent the conversion of pyruvate to lactate. Samples can be deproteinized by 10 Kd cutoff spin filter to remove proteins.

If you need any further assistance in the future, please do not hesitate to contact me.

The assay kit ab65342 is capable of detecting pyruvate in human plasma. Please note, our products are for "Research Use Only" (RUO), and are not intended for diagnostic use. Also note that due to the presence of LDH in serum and plasma, care must be taken during sample processing to prevent the conversion of pyruvate to lactate. Samples can be deproteinized by 10 kDa cutoff spin filter (ab93349) to remove proteins.

Click here (or use the following: https://www.abcam.com/Pyruvate-Assay-Kit-ab65342.html).

Click here (or use the following: https://www.abcam.com/10kD-Spin-Column-ab93349.html).

Thank you for your message and for providing this further information.


I appreciate the time your customer hasspent on these experiments, and would like to offer a free of charge replacementor credit note in compensation (providing the product has been purchased in the last 180 days). In order to arrange this, I would appreciate if you could confirm your order number and date of purchase?

Thank you for your continued cooperation. I look forward to hearing from you with details of how you would like to proceed.

Thank you for your inquiry.

I can confirm that this kithasbeen tested with urine, but not especially with urine samples that have been frozen.

I can suggest to run preliminary experiments to check what the difference in your samples will be when used frozen or fresh. This is especially recommended if you have many samples. A preliminary experiment will make sure that all will go well when testing abig number of samples simultaneously.

I assume that if you use samples that were all frozen first, there should be no problem. I suggest to freeze the sample quickly andto avoidfreeze/thaw cycles.

Pyruvate is a chemical compound and will be the same in human and mouse.

I hope this information is helpful and wish you good luck with your research.

Thank you for contacting us.

The assay buffers in each of these kits is different. The buffers are optimized in order to get the best results. Please use the assay buffer for the sample, specific to each kit.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Thank you for contacting us.

I am sorry that this kit did not have correct information on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used.

Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.

The credit note ID is for your reference only and does not automatically guarantee the credit.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

Thank you for contacting Abcam regarding ab65342.


I have confirmed with the laboratory that this kit will measure 1-200uM Pyruvate, not up to 1mM.


I do apologize for this error on our website and have requested that this be updated immediately. Please contact me at your earliest convenience to arrange for a refund.

Thanks for contacting me. I have looked at these kits that we discussed and here are my thoughts: Malate Assay Kit (ab83391): This kit will only measure malate present in the sample. The enzyme used to produce the oxidized form of malate, the form that is detected by the probe in the kit, is considered proprietary by our supplying lab so unfortunately I can't provide any information regarding the oxidized form of malate. Maltose Assay Kit (ab83388): This kit could be used to measure alpha-D-glucosidase activity by measuring the conversion of maltose to 2 glucose molecules. Amylase Assay Kit (ab102523): This kit could be used to measure alpha-amylase activity by measuring the specific cleavage of ethylidene-pNP-G7 substrate. Pyruvate Assay Kit (ab65342): This kit could be used to measure pyruvate oxidase activity by measuring the oxidation of pyruvate present in the reaction. PicoProbe™ Acetyl CoA Assay Kit (ab87546): This kit can only be used to measure free acetyl CoA present in biological samples. I hope this is helpful. Please let me know if you need any further information.

Thank you for contacting us and sorry for the delay in getting back to you. There is no stipulation as to what kind of 96 well plates are used as long as the detection method to be used is compatible. For example, if choosing to measure fluorescence black plates are needed, or if measuring luninescence or absorbance, white plates or clear plates would be most appropriate respectively. I hope this information has been of help. If you need any further information please do not hesitate to ask.

Thank you for contacting Abcam regarding ab65342. I have confirmed with the laboratory that cell culture media supernatants should be compatible with this kit. The supernatants can be directly tested with this kit, so no further sample preparation is necessary. I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.