Pyruvate Carboxylase Activity Assay Kit (Colorimetric) (ab289840)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Colorimetric
- Platform: Microplate
- Sample type: Cell Lysate, Tissue Homogenate
- Sensitivity: 200 µU
Overview
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Product name
Pyruvate Carboxylase Activity Assay Kit (Colorimetric) -
Detection method
Colorimetric -
Sample type
Cell Lysate, Tissue Homogenate -
Assay type
Enzyme activity (quantitative) -
Sensitivity
< 200 µU -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mammals -
Product overview
This Pyruvate Carboxylase Activity Assay Kit (ab289840, K2075) provides a facile, rapid way to measure PC activity in various biological samples. In this kit, pyruvate is carboxylated to OAA, which is further detected by the oxidation of NADH. Therefore, a decrease in NADH absorbance at 340 nm is proportional to the amount of PC in samples. The assay is simple, sensitive, and can detect PC activity lower than 200 μU in samples.
This kit provides a facile, rapid way to measure PC activity in various biological samples. In this kit, pyruvate is carboxylated to OAA, which is further detected by the oxidation of NADH. Therefore, a decrease in NADH absorbance at 340 nm is proportional to the amount of PC in samples. The assay is simple, sensitive, and can detect PC activity lower than 200 μU in samples.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests ATP II 1 vial NADH II 1 vial PC Developer Mix 1 vial PC Positive Control 1 x 10µl PC Substrate I 1 x 200µl Pyruvate Carboxylase Assay Buffer 1 x 25ml Substrate IV 1 vial -
Research areas
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Function
Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. Catalyzes in a tissue specific manner, the initial reactions of glucose (liver, kidney) and lipid (adipose tissue, liver, brain) synthesis from pyruvate. -
Pathway
Carbohydrate biosynthesis; gluconeogenesis. -
Involvement in disease
Defects in PC are the cause of pyruvate carboxylase deficiency (PC deficiency) [MIM:266150]. PC deficiency leads to lactic acidosis, mental retardation and death. It occurs in three forms: mild or type A, severe neonatal or type B, and a very mild lacticacidemia. -
Sequence similarities
Contains 1 ATP-grasp domain.
Contains 1 biotin carboxylation domain.
Contains 1 biotinyl-binding domain.
Contains 1 carboxyltransferase domain. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Alternative names
- mitochondrial
- OTTHUMP00000235155
- OTTHUMP00000235156
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab289840 has not yet been referenced specifically in any publications.