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    products/assay-kits/triglyceride-assay-kit-quantification-ab65336.pdf

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Triglyceride Assay Kit - Quantification (ab65336)

  • Datasheet
  • SDS
  • Protocol Booklet
Reviews (4)Q&A (25)References (428)

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Functional studies - ab65336
  • Triglyceride Quantification Kit (ab65336)
  • Triglyceride Quantification Kit (ab65336)
  • Functional Studies - Triglyceride Quantification Kit (ab65336)

Key features and details

  • Assay type: Quantitative
  • Detection method: Colorimetric/Fluorometric
  • Platform: Microplate reader
  • Assay time: 1 hr 20 min
  • Sample type: Cell Lysate, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
  • Sensitivity: 2 µM

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Overview

  • Product name

    Triglyceride Assay Kit - Quantification
    See all Triglyceride kits
  • Detection method

    Colorimetric/Fluorometric
  • Sample type

    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate
  • Assay type

    Quantitative
  • Sensitivity

    > 2 µM
  • Assay time

    1h 20m
  • Product overview

    Triglyceride Assay Kit (ab65336) is a sensitive, easy assay to measure triglyceride concentration in mammalian samples. In the triglyceride assay protocol, triglycerides are converted to free fatty acids and glycerol. Glycerol is then oxidized to generate a product which reacts with a probe to generate color (spectrophotometry at λ= 570 nm) and fluorescence (Ex/Em = 535/587 nm).


    Triglyceride assay protocol summary: 
    - add samples and standards to wells
    - add assay buffer and lipase, and incubate for 20 min
    - add triglyceride reaction mix and incubate for 60 min
    - analyze with microplate reader


    Please note: the general range is 0-10 nmol (colorimetric) and 0-1 nmol (fluorometric).


    If your sample contains reducing substances, they are likely to interfere with the assay. In this case, we recommend using Triglyceride Assay Kit (Fluorometric, Reducing samples) ab178780.


    Review our Metabolism Assay Guide to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.

  • Notes

    This product is manufactured by BioVision, an Abcam company and was previously called K622 Triglyceride Quantification Colorimetric/Fluorometric Kit. K622-100 is the same size as the 100 test size of ab65336.

    How other researchers have used Triglyceride Assay Kit ab65336

    The Triglyceride assay kit has been used in publications in a variety of sample types, including:
    - Human: serum1, plasma2, mammary epithelial and mammary cancer cell line lysate3, Huh7.5 hepatocyte-derived cell line lysate4, primary liver cell line lysates5, sebocyte cell culture lysates6
    - Mouse: hepatocyte cell lysates7, liver extract8, serum9, plasma10, kidney extracts11, liver tissue and serum12, cardiac tissue extracts13
    - Rat: liver tissue extract14, plasma15
    - Drosophila16

    References: 1-Huang Y et al. 2019, 2-Wilson et al 2018, 3-Yen MC et al. 2019, 4-Kim D et al. 2018, 5-Boteon Y et al. 2018,  6-Jin S and Lee MY 2018, 7-Zhang W et al. 2019, 8-Brial F et al. 2019, 9-Liu J et al. 2018 and Patton A et al. 2018, 10-Fernandes et al. 2018 and Körholz JC et al 2018 and Liang et al. 2018, 11-Ding W et al. 2018, 12-Cui XB et al. 2018, 13-Rohm M et al. 2018,  14-Yu S et al. 2018, 15-García-Ruiz et al. 2018, 16-Wen CA and Ballard JWO 2019

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests 2000 tests
    Lipase Blue 1 vial 20 vials
    Triglyceride Assay Buffer 1 x 25ml 20 x 25ml
    Triglyceride Enzyme Mix (lyophilized) Green 1 vial 20 vials
    Triglyceride Probe (in DMSO, anhydrous) Red 1 x 200µl 20 x 200µl
    Triglyceride Standard (1 mM) Yellow 1 x 300µl 20 x 300µl
  • Research areas

    • Kits/ Lysates/ Other
    • Kits
    • Cell Metabolism Kits
    • Fatty Acid Assays
    • Kits/ Lysates/ Other
    • Kits
    • Cell Metabolism Kits
    • Lipid Metabolism Kits
    • Fatty acids
    • Kits/ Lysates/ Other
    • Kits
    • Cell Metabolism Kits
    • Lipid Metabolism Kits
    • Obesity
    • Metabolism
    • Types of disease
    • Metabolic disorders
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Fatty acid oxidation
  • Relevance

    Triglycerides are the main constituent of vegetable oil, animal fat, LDL and VLDL, and play an important role as transporters of fatty acids as well as serving as an energy source. Triglycerides are broken down into fatty acids and glycerol, after which both can serve as substrates for energy producing and metabolic pathways. High blood levels of triglycerides are implicated in atherosclerosis, heart disease and stroke as well as in pancreatitis.
  • Alternative names

    • TG
    • Triacylglyceride
    • triglycerides

Associated products

  • Related Products

    • Triglyceride Standard (ab103967)
    • Enzyme Mix for Triglyceride Quantification Kit (ab155160)
    • Lipase for Triglyceride Quantification Kit (ab89001)

Images

  • Functional studies - ab65336
    Functional studies - ab65336Image from Samuel P et al., PLoS One 8(1), Fig 7b. doi: 10.1371/journal.pone.0048425. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Hepatic triglyceride levels was measured using ab65336 in male and female wild-type (WT) or AT2KO (knockout) mice with either normal diet (ND) or high fat diet (HFD). 

  • Triglyceride Quantification Kit (ab65336)
    Triglyceride Quantification Kit (ab65336)

    Fluorometric triglyceride standard curve: mean of duplicates (+/- SD) with background reads subtracted

  • Triglyceride Quantification Kit (ab65336)
    Triglyceride Quantification Kit (ab65336)

    Triglyceride measured in cell culture lysates showing quantity (nmol) per 1 mln cells.

    Samples with the concentration of 1e7 cells/mL were used. Samples were diluted 40-80 fold and measured fluorometrically.

    HepG2 cells were treated with 25 uM Chloroquine for 72h.

  • Functional Studies - Triglyceride Quantification Kit (ab65336)
    Functional Studies - Triglyceride Quantification Kit (ab65336)

    Colorimetric triglyceride standard curve using ab65336.

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (428)

Publishing research using ab65336? Please let us know so that we can cite the reference in this datasheet.

ab65336 has been referenced in 428 publications.

  • Hu MJ  et al. Acetylation of H3K27 activated lncRNA NEAT1 and promoted hepatic lipid accumulation in non-alcoholic fatty liver disease via regulating miR-212-5p/GRIA3. Mol Cell Biochem 477:191-203 (2022). PubMed: 34652536
  • Wang G & Dong J Network pharmacology approach to evaluate the therapeutic effects of mulberry leaf components for obesity. Exp Ther Med 23:56 (2022). PubMed: 34917182
  • Kim Y  et al. DA-1241, a Novel GPR119 Agonist, Improves Hyperglycaemia by Inhibiting Hepatic Gluconeogenesis and Enhancing Insulin Secretion in Diabetic Mice. Diabetes Metab J 46:337-348 (2022). PubMed: 35052026
  • Khalifa O  et al. Exendin-4 alleviates steatosis in an in vitro cell model by lowering FABP1 and FOXA1 expression via the Wnt/-catenin signaling pathway. Sci Rep 12:2226 (2022). PubMed: 35140289
  • Xu M  et al. The E3 ubiquitin-protein ligase Trim31 alleviates non-alcoholic fatty liver disease by targeting Rhbdf2 in mouse hepatocytes. Nat Commun 13:1052 (2022). PubMed: 35217669
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
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1-10 of 29 Abreviews or Q&A

Awesome Experience

Excellent Good 4/5 (Ease of Use)
Abreviews
Abreviews
The kits were easy to use. You are going to protocol with the packages when they send it to you.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 17 2020

Triglycerides from mouse plasma

Good Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
abreview image
Mouse blood was obtained by cardiac puncture in heparinized tubes and centrifuged at 13000 rpm for 2 min at 4 degrees. Plasma was subsequently diluted 1:20 in PBS for testing. This kit is easy to use and fast, and gives blood triglyceride concentrations in line with what is reported by literature.
Our experience gives reproducible results across the different kit lots.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Sep 03 2018

Question

Can we use either Tween-20 or Triton-X instead of NP-40 with this kit?

Read More

Abcam community

Verified customer

Asked on Mar 20 2017

Answer

We prefer NP40 to Triton or tween in this assay. NP-40 works better to keep lipids in solution and does not create background in the assay.

Read More

Abcam Scientific Support

Answered on Mar 20 2017

Question

What is the difference between ab178780 and ab65336?

Read More

Abcam community

Verified customer

Asked on Sep 01 2015

Answer

The picoprobe allows ab178780 to be more sensitive than the ab65336. ab178780 detects triglycerides <0.4uM whereas ab65336 can detect 2uM-10mM triglycerides.

I hope this helps!

Read More

Jared Kaminski

Abcam Scientific Support

Answered on Sep 01 2015

Question

What is the source of the triglyceride standard? What is the source of the lipase?

Read More

Abcam community

Verified customer

Asked on Jun 11 2013

Answer

The lipase is from a bacterial source and the standards are from plant oils.

Read More

Abcam Scientific Support

Answered on Jun 11 2013

Question

Can plasma samples be applied directly? Protocol booklet shows only serum sample.

Read More

Abcam community

Verified customer

Asked on Sep 03 2012

Answer

Yes, I can confirm that there should be no problem to treat the plasma samples in the same way as the serum, directly applying it.

Read More

Abcam Scientific Support

Answered on Sep 03 2012

Question

1) I found a white upper top layer after centrifugation to remove insoluble material from liver samples. Should I remove it or not?
2) SV is the sample volume (before dilution) added in sample wells (µl). My samples were diluted 10 fold with water before the assay. To get values are within range, these samples were diluted (60 fold) with Triglyceride assay buffer. Is the SV mean calculated before 60 fold dilution or after? When I calculate the final value, do I need to include the initial 10 fold dilution?

Read More

Abcam community

Verified customer

Asked on May 01 2012

Answer

1) The insoluble top layer may contain the triglyceride you wish to measure so please make sure to solubilize as much as possible (without losing any material) and to follow the recommendations (ie. repeated heating steps).
2) SV is indeed the abbreviation for sample volume. For calculating the final results, you need to take into account all the dilution steps you have made during the procedure so dilutions factors (10 and 60 fold need to be included in the calculation).

Read More

Abcam Scientific Support

Answered on May 01 2012

Question

Would like to know whether a plate is provided together with this kit or can we use any normal 96 well plate?

Read More

Abcam community

Verified customer

Asked on Apr 11 2012

Answer

The 96 well plate is not provided with the kit. Please use a black plate (clear bottom) for fluorescence and clear plate for colorimetric analysis.

Read More

Abcam Scientific Support

Answered on Apr 11 2012

Question

Does the Triglyceride Standard provided in this kit have a known molar mass?

Read More

Abcam community

Verified customer

Asked on Dec 07 2011

Answer

The standard provided with ab65336 has a molecular weight if 885.43 g/mol. 

Read More

Abcam Scientific Support

Answered on Dec 07 2011

Question

The values obtained from Triglyceride quantification kit (ab65336 are presented as a µmol/ml. How can I convert µmol/ml to mg/g tissue?

Read More

Abcam community

Verified customer

Asked on Jul 28 2011

Answer

1 mol/L is represented as 1mM, E.g. 50 mM is 50 mmol/L.

Divide by the molecular weight of the substance (average triglyceride molecular weight in your sample) to convert from moles to grams. Then mM/mol weight can be converted to get mg/L.

For this assay, since it is difficult to say the molar mass of triglyceride, unless which specific one is present in the sample is known, the accepted most common way of reporting the data is as mM or µmol/ml.

Read More

Abcam Scientific Support

Answered on Jul 28 2011

1-10 of 29 Abreviews or Q&A

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