Triglyceride Assay Kit - Quantification (ab65336)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 1 hr 20 min
- Sample type: Cell Lysate, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 2 µM
Overview
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Product name
Triglyceride Assay Kit - Quantification
See all Triglyceride kits -
Detection method
Colorimetric/Fluorometric -
Sample type
Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate -
Assay type
Quantitative -
Sensitivity
> 2 µM -
Assay time
1h 20m -
Product overview
Triglyceride Assay Kit (ab65336) is a sensitive, easy assay to measure triglyceride concentration in mammalian samples. In the triglyceride assay protocol, triglycerides are converted to free fatty acids and glycerol. Glycerol is then oxidized to generate a product which reacts with a probe to generate color (spectrophotometry at λ= 570 nm) and fluorescence (Ex/Em = 535/587 nm).
Triglyceride assay protocol summary:
- add samples and standards to wells
- add assay buffer and lipase, and incubate for 20 min
- add triglyceride reaction mix and incubate for 60 min
- analyze with microplate reader
Please note: the general range is 0-10 nmol (colorimetric) and 0-1 nmol (fluorometric).
If your sample contains reducing substances, they are likely to interfere with the assay. In this case, we recommend using Triglyceride Assay Kit (Fluorometric, Reducing samples) ab178780.
Target background
Triglycerides are the main constituent of vegetable oil, animal fat, LDL and VLDL, and play an important role as transporters of fatty acids as well as serving as an energy source. Triglycerides are broken down into fatty acids and glycerol, after which both can serve as substrates for energy producing and metabolic pathways. High blood levels of triglycerides are implicated in atherosclerosis, heart disease and stroke as well as in pancreatitis.
Review our Metabolism Assay Guide to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K622 Triglyceride Quantification Colorimetric/Fluorometric Kit. K622-100 is the same size as the 100 test size of ab65336.
How other researchers have used Triglyceride Assay Kit ab65336
The Triglyceride assay kit has been used in publications in a variety of sample types, including:
- Human: serum1, plasma2, mammary epithelial and mammary cancer cell line lysate3, Huh7.5 hepatocyte-derived cell line lysate4, primary liver cell line lysates5, sebocyte cell culture lysates6
- Mouse: hepatocyte cell lysates7, liver extract8, serum9, plasma10, kidney extracts11, liver tissue and serum12, cardiac tissue extracts13
- Rat: liver tissue extract14, plasma15
- Drosophila16References: 1-Huang Y et al. 2019, 2-Wilson et al 2018, 3-Yen MC et al. 2019, 4-Kim D et al. 2018, 5-Boteon Y et al. 2018, 6-Jin S and Lee MY 2018, 7-Zhang W et al. 2019, 8-Brial F et al. 2019, 9-Liu J et al. 2018 and Patton A et al. 2018, 10-Fernandes et al. 2018 and Körholz JC et al 2018 and Liang et al. 2018, 11-Ding W et al. 2018, 12-Cui XB et al. 2018, 13-Rohm M et al. 2018, 14-Yu S et al. 2018, 15-García-Ruiz et al. 2018, 16-Wen CA and Ballard JWO 2019
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests 2000 tests Lipase Blue 1 vial 20 vials Triglyceride Assay Buffer 1 x 25ml 20 x 25ml Triglyceride Enzyme Mix (lyophilized) Green 1 vial 20 vials Triglyceride Probe (in DMSO, anhydrous) Red 1 x 200µl 20 x 200µl Triglyceride Standard (1 mM) Yellow 1 x 300µl 20 x 300µl -
Research areas
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Alternative names
- TG
- Triacylglyceride
- triglycerides
Associated products
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Related Products
Images
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Functional studies - ab65336Image from Samuel P et al., PLoS One 8(1), Fig 7b. doi: 10.1371/journal.pone.0048425. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Hepatic triglyceride levels was measured using ab65336 in male and female wild-type (WT) or AT2KO (knockout) mice with either normal diet (ND) or high fat diet (HFD).
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Triglyceride Quantification Kit (ab65336)Fluorometric triglyceride standard curve: mean of duplicates (+/- SD) with background reads subtracted
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Triglyceride Quantification Kit (ab65336)Triglyceride measured in cell culture lysates showing quantity (nmol) per 1 mln cells.
Samples with the concentration of 1e7 cells/mL were used. Samples were diluted 40-80 fold and measured fluorometrically.
HepG2 cells were treated with 25 uM Chloroquine for 72h.
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Functional Studies - Triglyceride Quantification Kit (ab65336)Colorimetric triglyceride standard curve using ab65336.
Datasheets and documents
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SDS download
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Datasheet download
References (449)
ab65336 has been referenced in 449 publications.
- von Bülow V et al. Metabolic reprogramming of hepatocytes by Schistosoma mansoni eggs. JHEP Rep 5:100625 (2023). PubMed: 36590323
- Ho CK et al. LRG-1 promotes fat graft survival through the RAB31-mediated inhibition of hypoxia-induced apoptosis. J Cell Mol Med 26:3153-3168 (2022). PubMed: 35322540
- Abbas AO et al. Citrullus colocynthis Seed Ameliorates Layer Performance and Immune Response under Acute Oxidative Stress Induced by Paraquat Injection. Animals (Basel) 12:N/A (2022). PubMed: 35454193
- Nickel S et al. Human Mesenchymal Stromal Cells Resolve Lipid Load in High Fat Diet-Induced Non-Alcoholic Steatohepatitis in Mice by Mitochondria Donation. Cells 11:N/A (2022). PubMed: 35681524
- Sugimoto S et al. Brown adipose tissue-derived MaR2 contributes to cold-induced resolution of inflammation. Nat Metab 4:775-790 (2022). PubMed: 35760872