Von Kossa Stain Kit (Calcium Stain) (ab150687)
Overview
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Product name
Von Kossa Stain Kit (Calcium Stain)
See all Calcium kits -
Product overview
The Von Kossa (Calcium Stain) is intended for use in the histological visualization of calcium deposits in paraffin sections. This method is not specific for calcium itself but tissues are treated with a silver nitrate solution and the silver is deposited by replacing the calcium reduced by the strong light, and so can be visualized as metallic silver.
Other products for staining tissue sections
Find more kits and reagents in the special stains guide, or products for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
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Notes
Staining Interpretation
Calcium in mass deposits Black Calcium in dispersed deposits Gray Nuclei Red Cytoplasm Light Pink Control Tissue: Any paraffin-embedded tissue that contains calcium deposits.
To prepare bone section without decalcification, the resin is recommended instead of paraffin.
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 100 tests Nuclear Fast Red Solution (Enhanced) 1 x 125ml Silver Nitrate Solution 1 x 125ml Sodium Thiosulfate Solution (5%) 1 x 125ml -
Research areas
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Relevance
Calcium is essential for all living organisms, where Ca2+ sequestration and release into and out of the cytoplasm functions as a signal for many cellular processes. 99% of calcium is found in bones and teeth with the remaining 1% found in the blood and soft tissue. Serum calcium levels are tightly controlled (8.4-11.4 mg/dL) and any variation outside this range can have serious effects. Calcium plays a role in mediating the constriction and relaxation of blood vessels, nerve impulse transmission, muscle contraction, and hormone secretion. Calcium ion channels control the migration of calcium ions across cell membranes, permitting the activation and inhibition of a wide variety of enzymes. Causes of low calcium levels include chronic kidney failure, vitamin D deficiency, and low blood magnesium levels that can occur in severe alcoholism. -
Alternative names
- Ca++
- Ca2+
Images
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ab150687 - Von Kossa Stain Kit (Calcium Stain)
ab150687 Von Kossa Stain Kit (Calcium Stain) staining formalin-fixed-paraffin embedded human liver.
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ab150687 - Von Kossa Stain Kit (Calcium Stain)Staining of Bone using ab150687 - Calcium Stain Kit (Modified Von Kossa).
Datasheets and documents
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SDS download
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Datasheet download
References (33)
ab150687 has been referenced in 33 publications.
- Zakharchenko A et al. Poly-2-methyl-2-oxazoline-modified bioprosthetic heart valve leaflets have enhanced biocompatibility and resist structural degeneration. Proc Natl Acad Sci U S A 119:N/A (2022). PubMed: 35131859
- Tóth A et al. Daprodustat Accelerates High Phosphate-Induced Calcification Through the Activation of HIF-1 Signaling. Front Pharmacol 13:798053 (2022). PubMed: 35222025
- Rduch T et al. The Role of Inorganics in Preeclampsia Assessed by Multiscale Multimodal Characterization of Placentae. Front Med (Lausanne) 9:857529 (2022). PubMed: 35433726
- Bonatto Paese CL et al. Pharmacological intervention of the FGF-PTH axis as a potential therapeutic for craniofacial ciliopathies. Dis Model Mech 15:N/A (2022). PubMed: 35818799
- Martinez JS et al. High resolution DLP stereolithography to fabricate biocompatible hydroxyapatite structures that support osteogenesis. PLoS One 17:e0272283 (2022). PubMed: 35939440