WST-1 Assay Reagent - Cell Proliferation (ready to use) (ab155902)
Key features and details
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 30 min
- Sample type: Adherent cells, Suspension cells
Product nameWST-1 Assay Reagent - Cell Proliferation (ready to use)
See all Cell viability/proliferation kits
Sample typeAdherent cells, Suspension cells
Assay time0h 30m
WST-1 Assay Reagent ab155902 provides a simple, accurate and ready-to-use assay to measure cell proliferation, cell viability and cytotoxicity in mammalian cells.
The WST-1 assay protocol is based on the cleavage of the tetrazolium salt WST-1 to formazan by cellular mitochondrial dehydrogenases. The larger the number of viable cells, the higher the activity of the mitochondrial dehydrogenases, and in turn the greater the amount of formazan dye formed.
The WST-1 assay protocol is very simple:
- add the WST-1 assay reagent to the cell culture media and incubate for between 0.5 and 4 hrs
- shake the plate to mix the contents
- analyze the amount of formazan dye produced by measuring the absorbance at 440 nm
We also offer complete WST-1 assay kits: WST-1 assay kit ab65473 which is the full kit version of this reagent, and WST-1 assay kit ab65475 which uses an alternative WST-1 analog.
This product is manufactured by BioVision, an Abcam company and was previously called K304 Ready-to-use Cell Proliferation Colorimetric Reagent, WST-1. K304-2500 is the same size as the 2500 test size of ab155902.
WST-1 assays are non-radioactive, rapid and more sensitive than MTT, XTT, or MTS-based assays. The entire assay can be performed in the same microtiter plate and does not require extra steps like washing, harvesting and cell solubilization.
Review our cell health assays guide to learn more about our other cell viability, cytotoxicity and cell proliferation assay kits.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 2500 tests WST1 Reagent 1 x 2500 tests
RelevanceCell proliferation is the multiplication or reproduction of cells, as a result of cell growth and cell division, resulting in the expansion of a cell population.
- Cell Cytotoxicity
- cell tracking
Datasheets and documents
ab155902 has been referenced in 35 publications.
- Tripathi A et al. Pathogenic Mechanisms of Cytosolic and Membrane-Enriched a-Synuclein Converge on Fatty Acid Homeostasis. J Neurosci 42:2116-2130 (2022). PubMed: 35086904
- Zaki RM et al. Formulation of Chitosan-Coated Brigatinib Nanospanlastics: Optimization, Characterization, Stability Assessment and In-Vitro Cytotoxicity Activity against H-1975 Cell Lines. Pharmaceuticals (Basel) 15:N/A (2022). PubMed: 35337145
- Hou CP et al. The Upregulation of Caffeic Acid Phenethyl Ester on Growth Differentiation Factor 15 Inhibits Transforming Growth Factor ß/Smad Signaling in Bladder Carcinoma Cells. Biomedicines 10:N/A (2022). PubMed: 35884930
- Yu Z et al. New, potent, small molecule agonists of tyrosine kinase receptors attenuate dry eye disease. Front Med (Lausanne) 9:937142 (2022). PubMed: 36091713
- Serag E et al. Electrospun non-wovens potential wound dressing material based on polyacrylonitrile/chicken feathers keratin nanofiber. Sci Rep 12:15460 (2022). PubMed: 36104428