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Zinc Assay Kit (ab102507)

Reviews (1)Q&A (10)References (13)
Functional Studies - Zinc Assay Kit (ab102507)
  • Functional Studies - Zinc Quantification Kit (ab102507)
  • Functional Studies - Zinc Quantification Kit (ab102507)
  • Functional Studies - Zinc Quantification Kit (ab102507)
  • Example of Standard Curve

Key features and details

  • Assay type: Quantitative
  • Detection method: Colorimetric
  • Platform: Microplate reader
  • Assay time: 15 min
  • Sample type: Cell culture media, Cell Lysate, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
  • Sensitivity: 1 µg/ml

Overview

  • Product name

    Zinc Assay Kit
    See all Zinc kits

  • Detection method

    Colorimetric

  • Sample type

    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media

  • Assay type

    Quantitative

  • Sensitivity

    1 µg/ml

  • Assay time

    0h 15m

  • Product overview

    Zinc Assay Kit ab102507 is a convenient colorimetric assay in which Zinc binds to a ligand with development of absorbance at 560 nm.


    The zinc assay can be used with biological samples such as serum, plasma, csf or urine with detection sensitivity 0.2 µg/ml (~1-3 µM).


    Zinc assay protocl summary:
    - add samples and standards to wells
    - add reaction mix and incubate for 10 min
    - analyze with a microplate reader

  • Notes

    This product is manufactured by BioVision, an Abcam company and was previously called K387 Zinc Colorimetric Assay Kit. K387-100 is the same size as the 100 test size of ab102507.

    Zinc, a metallic chemical element, symbol Zn and atomic number 30 is chemically similar to Magnesium due to its similar size and sole oxidation state of 2+. Zinc is an essential mineral of great biological significance, because many enzymes require it as an essential cofactor.

  • Platform

    Microplate reader

Properties

Images

  • Functional Studies - Zinc Assay Kit (ab102507)
    Functional Studies - Zinc Assay Kit (ab102507)Crawford, Aaron C et al., PLoS pathogens vol. 14,5 e1007013., Fig 2, doi:10.1371/journal.ppat.1007013
    Indicated strains were cultured in low zinc medium (SD0, pH ~4.7), exposed to 25 µM ZnSO4 and zinc acquisition determined at indicated time points by measuring how much zinc remained in the cell free supernatant. C. albicans wild type acquires all measurable zinc within 60 minute; zrt2Δ does not; complementation restored zinc acquisition to 68%. Experiment performed three times
  • Functional Studies - Zinc Quantification Kit (ab102507)
    Functional Studies - Zinc Quantification Kit (ab102507)

    Zinc measured in cell lysates showing quantity (nmol) per 1 mln of tested cells

  • Functional Studies - Zinc Quantification Kit (ab102507)
    Functional Studies - Zinc Quantification Kit (ab102507)

    Zinc measured in mouse tissue lysates showing quantity (nmol) per mg protein of tested sample

  • Functional Studies - Zinc Quantification Kit (ab102507)
    Functional Studies - Zinc Quantification Kit (ab102507)

    Standard curve (colourimetric) : mean of duplicates (+/- SD) with background substracted

  • Example of Standard Curve
    Example of Standard Curve

    Representative Standard Curve using ab102507.

     

References (13)

Publishing research using ab102507? Please let us know so that we can cite the reference in this datasheet.

ab102507 has been referenced in 13 publications.

  • Gaffke L  et al. Impaired ion homeostasis as a possible associate factor in mucopolysaccharidosis pathogenesis: transcriptomic, cellular and animal studies. Metab Brain Dis 37:299-310 (2022). PubMed: 34928474
  • Murata K  et al. Clinical Significance of Serum Zinc Levels on the Development of Sarcopenia in Cirrhotic Patients. Cancer Diagn Progn 2:184-193 (2022). PubMed: 35399181
  • Dera HA  et al. Melatonin attenuates cerebral hypoperfusion-induced hippocampal damage and memory deficits in rats by suppressing TRPM7 channels. Saudi J Biol Sci 29:2958-2968 (2022). PubMed: 35531206
  • Tuong ZK  et al. Resolving the immune landscape of human prostate at a single-cell level in health and cancer. Cell Rep 37:110132 (2021). PubMed: 34936871
  • Xu W  et al. Hypozincemia in COVID-19 Patients Correlates With Stronger Antibody Response. Front Immunol 12:785599 (2021). PubMed: 35058926
View all Publications for this product

Customer reviews and Q&As

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1-10 of 11 Abreviews or Q&A

Using extract from murine colon feces for zinc determination.

 Excellent Excellent 5/5 (Ease of Use)
Abreviews
abreview image
I used pools of sera and colon poo extract to assess the utility of the kit for different samples. These samples were from Apodemus sylvaticus mice.
Sample preparation:
1. Sera was defrosted after storage at -80C. Centrifuged defrosted sera at 12000 rpm for 4mins.
2. Avoided disrupting the pellet and pipetted off equal volumes of sera into a clean Eppendorf to form a pool.
3. Colon poo was also defrosted from -80C. I weighed the sample and multiplied this mass by 3, and added this volume of proteinase inhibitor solution (PIS). For 0.2g of colon poo, a volume of 600ul PIS was added.
a. PIS was prepared by dissolving one tablet of Complete Mini Protease Inhibitor Cocktail Tablets (Roche, 11836153001) in 10ml 1x PBS.
4. Vortexed poo and PIS for 5 minutes. Stood for an hour at room temperature. Centrifuged at 12000rpm for 5minutes. Pipetted equal volumes of supernatant into a clean Eppendorf to form the fecal extract pool.
5. Made the 0.5mM standard (2ul standard provided plus 198ul Dh20).
6. Made a master mix of TCA and serum, and TCA and fecal extract. Equal volumes of TCA and sample were added. Span these samples at maximum speed for 5 minutes. Both samples had pellets after spinning.
7. Added 4 parts zinc reagent 1 to 1 part of zinc reagent 2.
8. I tried different dilutions of sample into a 50ul or 25ul sample volume. The samples were considered to already be a 1:2 dilution with TCA. Total sample volumes were made up with dH2O. Dilutions are listed.
a. Sera 50ul volume, 1:2, 1:5, 1:10, 1:100.
b. Sera 25ul volume 2:5, 1:5, 1:50.
c. Fecal extract 50ul volume, 1:2, 1:5, 1:10, 1:100.
d. Fecal extract 25ul volume 2:5, 1:5, 1:50.
9. I added 200ul of zinc reagent mixture to each 50ul sample, and 100ul to each 25ul.
10. Incubated 10min before reading the 560nm absorbance.

Conclusions:
I was testing whether for a small volume of sample it is better to dilute the sample into a large volume or to have the sample at a higher concentration in a lower volume. Both volumes worked, but following assay protocol and using the larger volume gave higher readings. The poo pool had lower readings than the serum pool but was still detectable. For both poo and sera, volumes of 5ul or above gave good readings. From the standard curve (and averaged across dilutions), the concentration of each of the pools was 13uM for fecal extract in 125uL total well volume, or 43uM in 250uL well volume. Sera was 62uM in 125uL total well volume, or 75uM in 250uL. For more concentrated samples, total well volume affected the calculated concentration less.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Submitted Dec 20 2022

Question

What is the correlation between Zinc conc determined using Abcam method and reference method such as Atomic Absorption Spectroscopy?

Read More
Answer

Thank you for your patience with this.


I heard an update from a different member of our lab and they said thatthe correlation between Zinc concentration determined using Abcam method and reference method such as Atomic Absorption Spectroscopyis greater than 0.9 (>0.9).


I hope this information helps. Please contact us with any other questions.

Read More

Question

Thank you for the quickly reply! The kit is suitable for the ion Zn2+ but also ZnSO4 dissociate in acqueous solution to give Zn2+ and SO42-. Why the presence of the negative ion should interfere with the Zn detection?
Thank you
Best

Read More
Answer

Thank you for contacting us.

You have kindly asked in your last question "I need to quantify ZnSO4 in aqueous samples and I would like to know if the Kit is suitable also for the salt detection and not only for the ion Zn2+." My answer was "no" this kit cannot be used for salt detection however it is OK for Zn ion detection.

On the other hand we have never used this kit with aqueous sample so I am sorry we do not know if the kit components will behave in water as expected.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Question

I'm writing since I've some questions about your Product " Zinc Quantification Kit ab102507". I need to quantify ZnSO4 in acqueous samples and I would like to know if the Kit is suitable also for the salt detection and not only for the ion Zn2+.

If so can I have a quotation?

Thank you

Best regards

Read More
Answer

Thank you for contacting us.

The ab102507 kit based on the chemistry of exchange of electrons between ligand and Zn2+ ions. The ligand is oxidized with interaction of Zn2+ ions with the development of colour which has absorbance maxima at 560nm. I am afraid this reaction may not occur with ZnSo4 salt so this kit might not work.

I hope this information is nevertheless helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Question

The readout calls for measuring absorbance at 560nm, but our elisa plate reader does not have a 560nm setting. The closest one is 590nm. Can 590nm be used instead of 560nm?

Read More
Answer



Do you have more information about the range of wavelengths that are read when your plate reader is set to 590 nm? Most plate readers have flexibility in their band width of detection, which allows them to get the readout in a range of wavelengths without compromising the efficiency of detection. If your plate reader has a flexibility of ± 30, you can use the wavelength range of 530 to 590 nm for a 560 recommendation. But if not, the reading efficiency will be compromised.

Read More

Question

I bought your Zinc quantification Kit for my research and wish to ask for clarity on some few issues.

In calculating the Zinc concentration as stated in the Zinc Quantification protocol step 5, i am to divide the sample amount by the sample volume. In running my sample, i used 20ul of Serum and 30ul of deionized water.The Zinc reaction mixture was 200ul. I want to asked if the sample amount will be 20ul that's the amount of serum used or 50ul which includes the water.
I also wish to know about the reference range of Zinc concentration in serum of children aged 1-15yrs.

Thanks

Read More
Answer

Thank you for contacting us.

To calculate the concentration of Zn

Zn = Sa (is the sample amount from standard curve corresponding to the OD of sample) / volume of the samples (in your case it will be 20ul, if you start with 20 and 50 if start with 50ul).

I would suggest searching the Zn concentration in literature at http://www.pubmed.com

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Question

Thanks very much for your help. Could you confirm that it is ok to use EDTA in the lysis buffer. The manual says ‘Chelators such as EDTA will give artifactually low Zinc levels and should be avoided. Heparin, citrate and oxalate are acceptable anticoagulants.’ I know this applies to blood samples but I just wanted to check it’s ok to use EDTA in the lysis buffer for mammalian cell lines.

Also, do you know whether this assay measures total zinc, or just labile (available) zinc?

Many thanks

Read More
Answer

Thank you for your reply.

I apologize for this mistake. The lysis buffer is a standard lysis buffer that can be used for our kits, but you are correct, in this case EDTA should not be added.

Please omit EDTA when preparing the lysis buffer. It will alsolyse the tissue withoutcontaining EDTA.

I am happy to confirm that ab102507 will detect total zinc and not only free zinc.

I hope this information is helpful and wish you good luck for your experiments.

Read More

Question

Product code: 102507

Inquiry: I am interested in your Zinc Quantification kit (ab102507). Please can you tell me whether this kit can be used with mammalian cell lines. Also, the protocol says that the plate should be read at 560 nm. Our plate reader will read at 540 and 570 nm. Will either of these be ok? Thanks

Read More
Answer

Thank you for your inquiry.

I am happy to confirm that ab102507 can be used for mammalian cells. Please find the recommended lysis buffer for mammalian cells below:

Solutions and Reagents:

Note: Prepare solutions with Milli-Q or equivalently purified water.

Cell Lysis Buffer (1X):

20 mM Tris (pH 7.5)

150 mM NaCl

1 mM EDTA

1 mM EGTA

1% Triton X-100

2.5 mM sodium pyrophosphate

1 mM b-Glycerolphosphate

1 mM Na3VO4

1 µg/ml Leupeptin

Note: We recommend adding 1 mM PMSF before use.

Samples containing significant amounts of protein should be deproteinized by adding 50 μl of the 7% TCA solution to 50 μl of the sample.

Please get in contact with supplier of the plate reader you are using to find out if it can read 560nm. Sometimes these machines do have filters that are called +/- 10nm and then it should be possible. I can recommend to measure the samples at 560nm to achieve a good result. Maybe a lab close by has a plate reader that can read 560nm?

I hope this information is helpful and wish you good luck for your research.

Read More

Question

Can you provide the specific graphical plot along with corresponding statistical information. How was this statistical information? What method was used such a standard linear regression using least squares, a Passing Bablok protocol or a Deming regression. HOw many samples were compared?

Read More
Answer

Thank you for your reply.

The correlation experiment was done as a part of an external study and unfortunately the lab told me that further details were unavailable. However, they did say that 40 samples were compared.

I hope this information helps.

Read More

Question

I did not see any of the analytical performance parameters which I thought might be included in the assay protocol. Can you provide the following information:

1. What is the linear range of the assay?

2. What is Limit of Detection?

3. What is the recovery, as % of Zinc, demonstrated by the assay?

4. What is imprecision of the assay to include a) within day, b) between day

5. What is the correlation between Zinc conc determined using Abcam method and reference method such as Atomic Absorption Spectroscopy?

Read More
Answer

Thank you for your reply. Below you may find the answers to your questions.


1. What is the linear range of the assay? 0 - 306 uM (or 0 - 20 ug/ml)


2. What is Limit of Detection? ˜ 0.61 uM (or 0.04 ug/ml)

3. What is the recovery, as % of Zinc, demonstrated by the assay? Typically the standard curve exhibits % CV < 2 %

4. What is imprecision of the assay to include a) within day, b) between day? Not determined

5. What is the correlation between Zinc conc determined using Abcam method and reference method such as Atomic Absorption Spectroscopy? Not determined

I hope this information helps. Please contact us with any other questions.

Read More

1-10 of 11 Abreviews or Q&A

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