10-Hydroxycamptothecin, DNA topoisomerase I inhibitor (ab141071)
Key features and details
- DNA topoisomerase I inhibitor
- CAS Number: 19685-09-7
- Purity: > 99%
- Soluble in DMSO to 50 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
10-Hydroxycamptothecin, DNA topoisomerase I inhibitor -
Description
DNA topoisomerase I inhibitor -
Biological description
DNA topoisomerase I inhibitor (IC50 = 106 nM). Cytotoxic antitumour compound, more potent than camptothecin (ab120115). In vitro and in vivo activity against a broad spectrum of cancers.
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Purity
> 99% -
CAS Number
19685-09-7 -
Chemical structure
Properties
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Chemical name
(4S)-4-Ethyl-4,9-dihydroxy-1H-pyrano[3',4':6,7]indolizino[1,2-b]quinoline-3,14(4H,12H)-dione -
Molecular weight
364.36 -
Molecular formula
C20H16N2O5 -
PubChem identifier
4330531 -
Storage instructions
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in DMSO to 50 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Toxic, refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
CCC1(O)C=2C=C3c4nc5ccc(O)cc5cc4CN3C(=O)C=2COC1=O -
Source
Synthetic
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Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab141071 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| Notes |
|---|
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Functional Studies
Use at an assay dependent concentration. |
Images
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Chemical Structure - 10-Hydroxycamptothecin, DNA topoisomerase I inhibitor (ab141071)2D chemical structure image of ab141071, 10-Hydroxycamptothecin, DNA topoisomerase I inhibitor
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Immunocytochemistry/ Immunofluorescence - 10-Hydroxycamptothecin, DNA topoisomerase I inhibitor (ab141071)ab2893 staining γH2A.X in HeLa cells treated with 10-Hydroxycamptothecin (ab141071), by ICC/IF. Increase of γH2A.X nuclear expression correlates with increased concentration of 10-Hydroxycamptothecin, as described in literature.
The cells were incubated at 37°C for 6 hours in media containing different concentrations of ab141071 (10-Hydroxycamptothecin) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab2893 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab141071 has not yet been referenced specifically in any publications.