5,7-Dichlorokynurenic acid sodium salt, NMDA receptor glycine site antagonist (ab120254)
Key features and details
- NMDA receptor glycine site antagonist; water soluble
- CAS Number:
- Soluble in water to 10 mM and in 1 eq. NaOH to 100 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
5,7-Dichlorokynurenic acid sodium salt, NMDA receptor glycine site antagonist -
Description
NMDA receptor glycine site antagonist; water soluble -
Biological description
Potent NMDA receptor glycine site antagonist. Water soluble form.
Also available in simple stock solutions (ab146691) - add 1 ml of water to get an exact, ready-to-use concentration. -
Chemical structure
Properties
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Chemical name
5,7-Dichloro-4-hydroxyquinoline-2-carboxylic acid sodium salt -
Molecular weight
280.04 -
Molecular formula
C10H4Cl2NNaO3 -
PubChem identifier
44134672 -
Storage instructions
Store at Room Temperature. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in water to 10 mM and in 1 eq. NaOH to 100 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
O=C(O[Na])c1cc(O)c2c(Cl)cc(Cl)cc2n1 -
Source
Synthetic
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Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab120254 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| Notes |
|---|
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Functional Studies
Use at an assay dependent concentration. |
Images
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Chemical Structure - 5,7-Dichlorokynurenic acid sodium salt, NMDA receptor glycine site antagonist (ab120254)2D chemical structure image of ab120254, 5,7-Dichlorokynurenic acid sodium salt, NMDA receptor glycine site antagonist
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Functional Studies - 5,7-Dichlorokynurenic acid sodium salt, NMDA receptor glycine site antagonist (ab120254)ab12416 staining cGMP in SKNSH cells treated with 5,7-Dichlorokynurenic acid sodium salt (ab120254), by ICC/IF. Decrease in cGMP expression correlates with increased concentration of 5,7-Dichlorokynurenic acid sodium salt, as described in literature.
The cells were incubated at 37°C for 20 minutes in media containing different concentrations of ab120254 (5,7-Dichlorokynurenic acid sodium salt) in DMSO. Some samples where then further incubated with 15 µM NMDA (ab120052) for 5 minutes and all samples were fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab12416 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with D
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab120254 has not yet been referenced specifically in any publications.