Apocynin, NADPH-oxidase inhibitor (ab120615)
Key features and details
- Selective NADPH-oxidase inhibitor
- CAS Number: 498-02-2
- Soluble in DMSO to 100 mM and in ethanol to 100 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
Apocynin, NADPH-oxidase inhibitor -
Description
Selective NADPH-oxidase inhibitor -
Biological description
Selective NADPH-oxidase inhibitor (IC50 = 10 μM). Inhibits production of reactive oxygen species. Also elicits a range of in vitro and in vivo anti-inflammatory effects.
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CAS Number
498-02-2 -
Chemical structure
Properties
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Chemical name
4'-Hydroxy-3'-methoxyacetophenone -
Molecular weight
166.18 -
Molecular formula
C9H10O3 -
PubChem identifier
2214 -
Storage instructions
Store at Room Temperature. The product can be stored for up to 12 months. -
Solubility overview
Soluble in DMSO to 100 mM and in ethanol to 100 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
CC(=O)C1=CC(=C(C=C1)O)OC -
Source
Synthetic
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Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab120615 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| Notes |
|---|
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Functional Studies
Use at an assay dependent concentration. |
Images
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Chemical Structure - Apocynin, NADPH-oxidase inhibitor (ab120615)2D chemical structure image of ab120615, Apocynin, NADPH-oxidase inhibitor
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Immunocytochemistry/ Immunofluorescence - Apocynin, NADPH-oxidase inhibitor (ab120615)ab19534 staining glutathione in A549 cells treated with apocynin (ab120615), by ICC/IF. Increase in glutathione expression correlates with increased concentration of apocynin, as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120615 (apocynin) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab19534 (10 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. -
Functional Studies - Apocynin, NADPH-oxidase inhibitor (ab120615)Image from Wang T et al., PloS one., 9(3): e91063. Fig 6.; doi: 10.1371/journal.pone.0091063 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/A–B. Endothelial cells were transfected with Nef cDNA, incubated for further 6 hours and treated with apocynin (200 nM), trolox (200 nM), Nox2 inhibitor (1 uM) or IKKi (100 nM). After additional 18 hours supernatants were analyzed for Nef-induced MCP-1 production (A) and endothelial cells for apoptosis using TUNEL (B). C–D. Endothelial cells were cocultured with Nef-transfected Jurkat cells for 24 h, and then treated with apocynin (200 nM), trolox (200 nM), Nox2 inhibitor (1 uM) or IKKi (100 nM) and incubated an additional 18 h, then analyzed for Nef-induced MCP-1 production (C) and apoptosis of endothelial cells (D). Data were expressed as fold MCP-1 production and apoptosis, normalized to the mean of control measurements. Data represent mean±SD from 3 separate experiments in which measurements were made in triplicate. *P<0.05, and **P<0.01.
Wang T et al., PloS one., 9(3): e91063. Fig 6.; doi: 10.1371/journal.pone.0091063
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (5)
ab120615 has been referenced in 5 publications.
- Deng J et al. DDR1-induced neutrophil extracellular traps drive pancreatic cancer metastasis. JCI Insight 6:N/A (2021). PubMed: 34237033
- Qie X et al. Endoplasmic Reticulum Stress Mediates Methamphetamine-Induced Blood-Brain Barrier Damage. Front Pharmacol 8:639 (2017). PubMed: 28959203
- Sun Z et al. Inhibiting NADPH oxidase protects against long-term memory impairment induced by neonatal sevoflurane exposure in mice. Br J Anaesth 117:80-6 (2016). PubMed: 27147542
- Wang T et al. Transfer of intracellular HIV Nef to endothelium causes endothelial dysfunction. PLoS One 9:e91063 (2014). PubMed: 24608713
- McMillan SJ et al. Siglec-E promotes ß2-integrin-dependent NADPH oxidase activation to suppress neutrophil recruitment to the lung. J Biol Chem 289:20370-6 (2014). PubMed: 24895121