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    products/biochemicals/apocynin-nadph-oxidase-inhibitor-ab120615.pdf

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Biochemicals Chemical Type Biochemicals
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Apocynin, NADPH-oxidase inhibitor (ab120615)

  • Datasheet
  • SDS
  • COA
Submit a review Q&A (1)References (5)

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Chemical Structure - Apocynin, NADPH-oxidase inhibitor (ab120615)
  • Immunocytochemistry/ Immunofluorescence - Apocynin, NADPH-oxidase inhibitor (ab120615)
  • Functional Studies - Apocynin, NADPH-oxidase inhibitor (ab120615)

Key features and details

  • Selective NADPH-oxidase inhibitor
  • CAS Number: 498-02-2
  • Soluble in DMSO to 100 mM and in ethanol to 100 mM
  • Form / State: Solid
  • Source: Synthetic

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Overview

  • Product name

    Apocynin, NADPH-oxidase inhibitor
  • Description

    Selective NADPH-oxidase inhibitor
  • Biological description

    Selective NADPH-oxidase inhibitor (IC50 = 10 μM). Inhibits production of reactive oxygen species. Also elicits a range of in vitro and in vivo anti-inflammatory effects.

  • CAS Number

    498-02-2
  • Chemical structure

    Chemical Structure

Properties

  • Chemical name

    4'-Hydroxy-3'-methoxyacetophenone
  • Molecular weight

    166.18
  • Molecular formula

    C9H10O3
  • PubChem identifier

    2214
  • Storage instructions

    Store at Room Temperature. The product can be stored for up to 12 months.
  • Solubility overview

    Soluble in DMSO to 100 mM and in ethanol to 100 mM
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Refer to SDS for further information.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILES

    CC(=O)C1=CC(=C(C=C1)O)OC
  • Source

    Synthetic

  • Research areas

    • Biochemicals
    • Chemical Type
    • Biochemicals
    • Biochemicals
    • Pharmacology
    • Enzymes
    • Oxidases
    • NADPH oxidase
    • Inhibitors
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Oxidative stress
    • Biochemicals
    • Pharmacology
    • Signaling
    • Signal transduction
    • Nitric oxide & oxidative stress
    • ROS
    • Biochemicals
    • Research Area
    • Cancer
    • Signal transduction
    • Nitric oxide & oxidative stress
    • ROS
    • Biochemicals
    • Pharmacology
    • Signaling
    • Immunomodulators
    • Immunomodulators & Immunosuppressants

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab120615 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies
Use at an assay dependent concentration.
Notes
Functional Studies
Use at an assay dependent concentration.

Images

  • Chemical Structure - Apocynin, NADPH-oxidase inhibitor (ab120615)
    Chemical Structure - Apocynin, NADPH-oxidase inhibitor (ab120615)
    2D chemical structure image of ab120615, Apocynin, NADPH-oxidase inhibitor
  • Immunocytochemistry/ Immunofluorescence - Apocynin, NADPH-oxidase inhibitor (ab120615)
    Immunocytochemistry/ Immunofluorescence - Apocynin, NADPH-oxidase inhibitor (ab120615)
    ab19534 staining glutathione in A549 cells treated with apocynin (ab120615), by ICC/IF. Increase in glutathione expression correlates with increased concentration of apocynin, as described in literature.
    The cells were incubated at 37°C for 24h in media containing different concentrations of ab120615 (apocynin) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab19534 (10 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody.
  • Functional Studies - Apocynin, NADPH-oxidase inhibitor (ab120615)
    Functional Studies - Apocynin, NADPH-oxidase inhibitor (ab120615)Image from Wang T et al., PloS one., 9(3): e91063. Fig 6.; doi: 10.1371/journal.pone.0091063 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    A–B. Endothelial cells were transfected with Nef cDNA, incubated for further 6 hours and treated with apocynin (200 nM), trolox (200 nM), Nox2 inhibitor (1 uM) or IKKi (100 nM). After additional 18 hours supernatants were analyzed for Nef-induced MCP-1 production (A) and endothelial cells for apoptosis using TUNEL (B). C–D. Endothelial cells were cocultured with Nef-transfected Jurkat cells for 24 h, and then treated with apocynin (200 nM), trolox (200 nM), Nox2 inhibitor (1 uM) or IKKi (100 nM) and incubated an additional 18 h, then analyzed for Nef-induced MCP-1 production (C) and apoptosis of endothelial cells (D). Data were expressed as fold MCP-1 production and apoptosis, normalized to the mean of control measurements. Data represent mean±SD from 3 separate experiments in which measurements were made in triplicate. *P<0.05, and **P<0.01.

    Wang T et al., PloS one., 9(3): e91063. Fig 6.; doi: 10.1371/journal.pone.0091063

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download
  • COA

References (5)

Publishing research using ab120615? Please let us know so that we can cite the reference in this datasheet.

ab120615 has been referenced in 5 publications.

  • Deng J  et al. DDR1-induced neutrophil extracellular traps drive pancreatic cancer metastasis. JCI Insight 6:N/A (2021). PubMed: 34237033
  • Qie X  et al. Endoplasmic Reticulum Stress Mediates Methamphetamine-Induced Blood-Brain Barrier Damage. Front Pharmacol 8:639 (2017). PubMed: 28959203
  • Sun Z  et al. Inhibiting NADPH oxidase protects against long-term memory impairment induced by neonatal sevoflurane exposure in mice. Br J Anaesth 117:80-6 (2016). PubMed: 27147542
  • Wang T  et al. Transfer of intracellular HIV Nef to endothelium causes endothelial dysfunction. PLoS One 9:e91063 (2014). PubMed: 24608713
  • McMillan SJ  et al. Siglec-E promotes ß2-integrin-dependent NADPH oxidase activation to suppress neutrophil recruitment to the lung. J Biol Chem 289:20370-6 (2014). PubMed: 24895121

Customer reviews and Q&As

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Submit a review Submit a question

Question

Is this a reversible inhibitor?

Read More

Abcam community

Verified customer

Asked on Aug 15 2012

Answer

Thank you very much for your call today and for your question about ab120615.

I've looked through some literature and references, and apocynin does seem to reversibly inhibitNADPH oxidase:

http://www.hindawi.com/journals/mi/2008/106507/

http://hyper.ahajournals.org/content/40/5/755.full.pdf

http://cong35.sifweb.org/congresso_abs_view.php?id=576

Here is another study that might be of interest to you:

http://hyper.ahajournals.org/content/51/2/211.full.pdf

I hope that this information will be useful, but please let me know if you have any further questions and I'll be happy to help. Have a nice day!

Read More

Abcam Scientific Support

Answered on Aug 15 2012

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