ATP-gamma-S, Kinase substrate (ab138911)
Key features and details
- Kinase substrate
- CAS Number: 93839-89-5
- Soluble in water to 10 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
ATP-gamma-S, Kinase substrate -
Description
Kinase substrate -
Alternative names
- ATP gamma S
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General notes
Kinase reaction reagent to be used with ab92570 rabbit monoclonal to thiophosphate ester for the identification of direct kinase substrates as mentioned in Allen JJ et al. Nat Methods 4:511-6 (2007).
After the kinase of interest has accepted ATP-γ-S, p-Nitrobenzyl mesylate (ab138910) can be used to alkylate the thiophospholyation site on the substrates. A thiophosphate ester rabbit monoclonal antibody (ab92570) is introduced to identify the tagged substrates.
For western blot analysis, we used ATPγS or A*TPγS analogs at a concentration of 1 mM. For kinetic measurements, ATPγS or A*TPγS analog concentration varied from 0.1 μM to 250 μM. See Allen JJ et al. Nat Methods 4:511-6 (2007); Supp. Material. -
CAS Number
93839-89-5 -
Chemical structure
Properties
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Chemical name
Adenosine 5'-(3-thiotriphosphate) tetralithium salt -
Molecular weight
546.98 -
Molecular formula
C10H12Li4N5O12P3S -
PubChem identifier
5311341 -
Storage instructions
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in water to 10 mM -
Handling
This product is supplied in one (or more) pack size which is freeze dried. Therefore the contents may not be readily visible, as they can coat the bottom or walls of the vial. Please see our FAQs and information page for more details on handling.
Unstable; make up solutions fresh and use immediately.
Refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
[Li+].[Li+].[Li+].[Li+].C1=NC2=C(C(=N1)N)N=CN2[C@H]3[C@@H]([C@@H]([C@H](O3)COP(=O)([O-])OP(=O)([O-])OP(=S)([O-])[O-])O)O -
Source
Synthetic
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab138911 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| Notes |
|---|
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Functional Studies
Use at an assay dependent concentration. |
Images
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Chemical Structure - ATP-gamma-S, Kinase substrate (ab138911)2D chemical structure image of ab138911, ATP-gamma-S, Kinase substrate
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (22)
ab138911 has been referenced in 22 publications.
- Han H et al. The Hippo pathway kinases LATS1 and LATS2 attenuate cellular responses to heavy metals through phosphorylating MTF1. Nat Cell Biol 24:74-87 (2022). PubMed: 35027733
- Yildirim Z et al. Intercepting IRE1 kinase-FMRP signaling prevents atherosclerosis progression. EMBO Mol Med 14:e15344 (2022). PubMed: 35191199
- Zhong J et al. Structural basis of dynamic P5CS filaments. Elife 11:N/A (2022). PubMed: 35286254
- Yu CC et al. Sequential Phosphorylation of Hepatitis C Virus NS5A Protein Requires the ATP-Binding Domain of NS3 Helicase. J Virol 96:e0010722 (2022). PubMed: 35293767
- Yildirim AD et al. ER Stress-Induced Sphingosine-1-Phosphate Lyase Phosphorylation Potentiates the Mitochondrial Unfolded Protein Response. J Lipid Res 63:100279 (2022). PubMed: 36100091