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    products/biochemicals/bafilomycin-a1-v-atpase-inhibitor-ab120497.pdf

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Signal Transduction Metabolism Plasma Membrane ATPases
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Bafilomycin A1, (V)-ATPase inhibitor (ab120497)

  • Datasheet
  • SDS
  • COA
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Immunocytochemistry/ Immunofluorescence - Bafilomycin A1, (V)-ATPase inhibitor (ab120497)
  • Chemical Structure - Bafilomycin A1, (V)-ATPase inhibitor (ab120497)
  • Immunocytochemistry/ Immunofluorescence - Bafilomycin A1, (V)-ATPase inhibitor (ab120497)
  • Immunocytochemistry/ Immunofluorescence - Bafilomycin A1, (V)-ATPase inhibitor (ab120497)

Key features and details

  • Highly potent, selective (V)-ATPase inhibitor
  • CAS Number: 88899-55-2
  • Soluble in ethanol to 5mM
  • Form / State: Solid
  • Source: Streptomyces griseus

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Overview

  • Product name

    Bafilomycin A1, (V)-ATPase inhibitor
  • Description

    Highly potent, selective (V)-ATPase inhibitor
  • Biological description

    Highly potent, selective vacuolar (V)-ATPase inhibitor (IC50 = 500 pM in chromaffin granule membranes). Induces vacuolar deacidification and promotes apoptosis.

  • CAS Number

    88899-55-2
  • Chemical structure

    Chemical Structure

Properties

  • Chemical name

    (3Z,5E,7R,8S,9S,11E,13E,15S,16R)-8-Hydroxy-16-[(1S,2R,3S)-2-hydroxy-1-methyl-3-[(2R,4R,5S,6R)-tetrahydro-2,4-dihydroxy-5-methyl-6-(1-methylethyl)-2H-pyran-2-yl]butyl]-3,15-dimethoxy-5,7,9,11-tetramethyloxacyclohexadeca-3,5,11,13-tetraen-2-one
  • Molecular weight

    622.84
  • Molecular formula

    C35H58O9
  • PubChem identifier

    6436223
  • Storage instructions

    Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
  • Solubility overview

    Soluble in ethanol to 5mM
  • Handling

    This product is supplied in one (or more) pack size which is freeze dried. Therefore the contents may not be readily visible, as they can coat the bottom or walls of the vial. Please see our FAQs and information page for more details on handling.

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILES

    O[C@@]1(C[C@@H](O)[C@H](C)[C@H](O1)C(C)C)[C@@H](C)[C@H](O)[C@H](C)C2OC(=O)C(OC)=CC=C(C)[C@@H](C)[C@@H](O)[C@@H](C)CC(C)=CC=C[C@@H]2OC
  • Source

    Streptomyces griseus

  • Research areas

    • Signal Transduction
    • Metabolism
    • Plasma Membrane
    • ATPases
    • Biochemicals
    • Chemical Type
    • Biochemicals
    • Biochemicals
    • Pharmacology
    • Enzymes
    • ATPase & GTPase
    • Inhibitors

Images

  • Immunocytochemistry/ Immunofluorescence - Bafilomycin A1, (V)-ATPase inhibitor (ab120497)
    Immunocytochemistry/ Immunofluorescence - Bafilomycin A1, (V)-ATPase inhibitor (ab120497)

    ab240635 staining SQSTM1/p62 (autophagosome) in control HeLa cells (left panel) and SQSTM1/p62 in HeLa cells treated with 100nM bafilomycin A1 (ab120497) for 18hrs (right panel). The cells were fixed with methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab240635 at 2ug/ml and ab7291 (Tubulin) at 1/1000 dilution overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor® 594) (ab150120) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.

    Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

  • Chemical Structure - Bafilomycin A1, (V)-ATPase inhibitor (ab120497)
    Chemical Structure - Bafilomycin A1, (V)-ATPase inhibitor (ab120497)
    2D chemical structure image of ab120497, Bafilomycin A1, (V)-ATPase inhibitor
  • Immunocytochemistry/ Immunofluorescence - Bafilomycin A1, (V)-ATPase inhibitor (ab120497)
    Immunocytochemistry/ Immunofluorescence - Bafilomycin A1, (V)-ATPase inhibitor (ab120497)

    ab155686 staining SQSTM1/p62 (autophagosome) in control HeLa cells (left panel) and SQSTM1/p62 in HeLa cells treated with 1uM bafilomycin A1 (ab120497) for 18hrs (right panel). The cells were fixed with methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab155686 at 1/500 dilution and ab7291 (Tubulin) at 1/1000 dilution overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor® 594) (ab150120) at 2 μg/ml (shown in pseudo color red).  Nuclear DNA was labelled in blue with DAPI.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Immunocytochemistry/ Immunofluorescence - Bafilomycin A1, (V)-ATPase inhibitor (ab120497)
    Immunocytochemistry/ Immunofluorescence - Bafilomycin A1, (V)-ATPase inhibitor (ab120497)

    ab109012 staining SQSTM1/p62 (autophagosome) in control HeLa cells (left panel) and SQSTM1/p62 in HeLa cells treated with 1uM bafilomycin A1 (ab120497) for 18hrs (right panel). The cells were fixed with methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab109012 at 5ug/ml and ab7291 (Tubulin) at 1/1000 dilution overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor® 594) (ab150120) at 2 μg/ml (shown in pseudo color red).  Nuclear DNA was labelled in blue with DAPI.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download
  • COA

References (45)

Publishing research using ab120497? Please let us know so that we can cite the reference in this datasheet.

ab120497 has been referenced in 45 publications.

  • Feng L  et al. HMGB1 downregulation in retinal pigment epithelial cells protects against diabetic retinopathy through the autophagy-lysosome pathway. Autophagy N/A:1-20 (2021). PubMed: 34024230
  • Qian C  et al. Localization, proteomics, and metabolite profiling reveal a putative vesicular transporter for UDP-glucose. Elife 10:N/A (2021). PubMed: 34269178
  • Li W  et al. AKR1B10 negatively regulates autophagy through reducing GAPDH upon glucose starvation in colon cancer. J Cell Sci 134:N/A (2021). PubMed: 33758077
  • Lee JH  et al. n-Butylidenephthalide Modulates Autophagy to Ameliorate Neuropathological Progress of Spinocerebellar Ataxia Type 3 through mTOR Pathway. Int J Mol Sci 22:N/A (2021). PubMed: 34199295
  • Kim D  et al. Apoptotic Cells Trigger Calcium Entry in Phagocytes by Inducing the Orai1-STIM1 Association. Cells 10:N/A (2021). PubMed: 34685684
View all Publications for this product

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