BAPTA-AM, Ca2+ chelator (ab120503)
Key features and details
- Selective Ca2+ chelator. Analog of BAPTA.
- CAS Number: 126150-97-8
- Soluble in DMSO to 100 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
BAPTA-AM, Ca2+ chelator -
Description
Selective Ca2+ chelator. Analog of BAPTA. -
Biological description
Selective Ca2+ chelator. Cell-permeable analog of BAPTA (ab120449). Useful for manipulation of intracellular free Ca2+ levels. Shows varied biological activity. Blocks hKv1.5, Kv11.1 (hERG) and hKv1.3 channels (Ki values are 1.23, 1.30 and 1.45 μM, respectively).
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CAS Number
126150-97-8 -
Chemical structure
Properties
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Chemical name
1,2-Bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid tetrakis(acetoxymethyl ester) -
Molecular weight
764.68 -
Molecular formula
C34H40N2O18 -
PubChem identifier
2293 -
Storage instructions
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in DMSO to 100 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information.
For more information on AM esters please visit our AM esters FAQ page.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
CC(=O)OCOC(=O)CN(CC(=O)OCOC(=O)C)C1=CC=CC=C1OCCOC2=CC=CC=C2N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C -
Source
Synthetic
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Research areas
Images
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Chemical Structure - BAPTA-AM, Ca2+ chelator (ab120503)2D chemical structure image of ab120503, BAPTA-AM, Ca2+ chelator
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Immunocytochemistry/ Immunofluorescence - BAPTA-AM, Ca2+ chelator (ab120503)ab66705 staining PAI1 in HepG2 cells treated with BAPTA-AM (ab120503), by ICC/IF. Increase in PAI1 expression correlates with increased concentration of BAPTA-AM, as described in literature.
The cells were incubated at 37°C for 4 hours in media containing different concentrations of ab120503 (BAPTA-AM) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab66705 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (26)
ab120503 has been referenced in 26 publications.
- Tomàs J et al. Muscarinic Receptors in Developmental Axonal Competition at the Neuromuscular Junction. Mol Neurobiol 60:1580-1593 (2023). PubMed: 36526930
- Alten B et al. Presynaptic mechanisms underlying GABAB-receptor-mediated inhibition of spontaneous neurotransmitter release. Cell Rep 38:110255 (2022). PubMed: 35045279
- NavaneethaKrishnan S et al. Cdk5 regulates IP3R1-mediated Ca2+ dynamics and Ca2+-mediated cell proliferation. Cell Mol Life Sci 79:495 (2022). PubMed: 36001172
- Tomiyama KI & Funada M Synthetic cannabinoid CP-55,940 induces apoptosis in a human skeletal muscle model via regulation of CB1 receptors and L-type Ca2+ channels. Arch Toxicol 95:617-630 (2021). PubMed: 33174160
- Alten B et al. Role of Aberrant Spontaneous Neurotransmission in SNAP25-Associated Encephalopathies. Neuron 109:59-72.e5 (2021). PubMed: 33147442