beta-Lapachone, Topoisomerase I inhibitor (ab141097)
Key features and details
- Topoisomerase I inhibitor
- CAS Number: 4707-32-8
- Purity: > 98%
- Soluble in ethanol to 50 mM and in DMSO to 100 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
beta-Lapachone, Topoisomerase I inhibitor -
Description
Topoisomerase I inhibitor -
Biological description
Topoisomerase I inhibitor. Inhibits topoisomerase I induced DNA cleavage. Induces apoptosis via a p53 independent mechanism. Shows antitumor, antiviral, antibacterial, antifungal and wound healing activities.
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Purity
> 98% -
CAS Number
4707-32-8 -
Chemical structure
Properties
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Chemical name
3,4-Dihydro-2,2-dimethyl-2H-naphtho[1,2-b]pyran-5,6-dione -
Molecular weight
242.27 -
Molecular formula
C15H14O3 -
PubChem identifier
497540 -
Storage instructions
Store at +4°C. The product can be stored for up to 12 months. -
Solubility overview
Soluble in ethanol to 50 mM and in DMSO to 100 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
O=C3C(=O)c1ccccc1C2OC(C)(C)CCC23 -
Source
Synthetic
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Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab141097 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| Notes |
|---|
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Functional Studies
Use at an assay dependent concentration. |
Images
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Chemical Structure - beta-Lapachone, Topoisomerase I inhibitor (ab141097)
2D chemical structure of ab141097, beta-Lapachone, Topoisomerase I inhibitor
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Functional Studies - beta-Lapachone, Topoisomerase I inhibitor (ab141097)ab18209 staining p21 in serum starved PC-3 cells treated with ß-lapachone (ab141097), by ICC/IF. Increase of p21 nuclear expression correlates with increased concentration of ß-lapachone, as described in literature.
The cells were incubated at 37°C for 6 hours in media containing different concentrations of ab141097 (ß-lapachone) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab18209 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (2)
ab141097 has been referenced in 2 publications.
- Steinmeier J et al. ß-Lapachone Induces Acute Oxidative Stress in Rat Primary Astrocyte Cultures that is Terminated by the NQO1-Inhibitor Dicoumarol. Neurochem Res 45:2442-2455 (2020). PubMed: 32789798
- Mokarizadeh N et al. ß-Lapachone attenuates cognitive impairment and neuroinflammation in beta-amyloid induced mouse model of Alzheimer's disease. Int Immunopharmacol 81:106300 (2020). PubMed: 32070922