For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    products/biochemicals/brdu-5-bromo-2-deoxyuridine-thymidine-analog-ab142567.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Epigenetics and Nuclear Signaling DNA / RNA DNA Synthesis Other
Share by email

BrdU (5-bromo-2'-deoxyuridine), Thymidine analog (ab142567)

  • Datasheet
  • SDS
  • COA
Reviews (1) Submit a question References (48)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunocytochemistry/ Immunofluorescence - BrdU (5-bromo-2'-deoxyuridine), Thymidine analog (ab142567)
  • Chemical Structure - BrdU (5-bromo-2'-deoxyuridine), Thymidine analog (ab142567)
  • Functional Studies - BrdU (5-bromo-2'-deoxyuridine), Thymidine analog (ab142567)

Key features and details

  • Thymidine analog for measuring cell proliferation
  • CAS Number: 59-14-3
  • Purity: > 98%
  • Soluble in water to 50 mM
  • Form / State: Solid
  • Source: Synthetic

You may also be interested in

Primary
Product image
Anti-TBR2 / Eomes antibody [EPR19012] (ab183991)
Primary
Product image
Alexa Fluor® 647 Anti-BrdU antibody [BU1/75 (ICR1)] (ab220075)
Primary
Product image
Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (ab6326)

View more associated products

Overview

  • Product name

    BrdU (5-bromo-2'-deoxyuridine), Thymidine analog
  • Description

    Thymidine analog for measuring cell proliferation
  • Purity

    > 98%
  • General notes

    Unstained control slides from mice treated with BrdU (formalin-fixed, paraffin-embedded intestine sections) are available as BrdU control slides ab129956. 

  • CAS Number

    59-14-3
  • Chemical structure

    Chemical Structure

Properties

  • Chemical name

    5-Bromo-1-[(2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]pyrimidine-2,4-dione
  • Molecular weight

    307.10
  • Molecular formula

    C9H11BrN2O5
  • PubChem identifier

    6035
  • Storage instructions

    Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
  • Solubility overview

    Soluble in water to 50 mM
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Toxic, refer to SDS for further information

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILES

    BrC1=CN(C(=O)NC1=O)[C@H]2CC(O)[C@@H](CO)O2
  • Source

    Synthetic

  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Synthesis
    • Other
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA / Nucleotides
    • Biochemicals
    • Product Range
    • Just Add Water
    • Biochemicals
    • Chemical Type
    • Biochemicals
    • Biochemicals
    • Chemical Type
    • Nucleoside
    • Kits/ Lysates/ Other
    • Tools and Reagents
    • Fluorescent dyes and reagents
    • Biochemicals
    • Pharmacology
    • Signaling
    • DNA, RNA & protein synthesis
    • DNA & RNA synthesis
    • Biochemicals
    • Chemical Type
    • Dyes, stains and fluorescent tools

Associated products

  • Related Products

    • BrdU Cell Proliferation ELISA Kit (colorimetric) (ab126556)
    • BrdU Cell Proliferation ELISA Kit (chemiluminescent) (ab126572)
    • Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (ab6326)

Images

  • Immunocytochemistry/ Immunofluorescence - BrdU (5-bromo-2'-deoxyuridine), Thymidine analog (ab142567)
    Immunocytochemistry/ Immunofluorescence - BrdU (5-bromo-2'-deoxyuridine), Thymidine analog (ab142567)

    ab220075 staining BrdU in HeLa cells. Untreated and BrdU treated (ab142567, 10uM for 24 hours) cells were fixed with 100% methanol (5 min) and then subjected to acid hydrolysis using 2M HCL in 0.1% PBS-Tween for 30 minutes at room temperature to denature the DNA. They were then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab220075 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

     

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

     

    This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 4% formaldehyde (10 min).

  • Chemical Structure - BrdU (5-bromo-2'-deoxyuridine), Thymidine analog (ab142567)
    Chemical Structure - BrdU (5-bromo-2'-deoxyuridine), Thymidine analog (ab142567)
    2D chemical structure image of ab142567, BrdU (5-bromo-2'-deoxyuridine), Thymidine analog
  • Functional Studies - BrdU (5-bromo-2'-deoxyuridine), Thymidine analog (ab142567)
    Functional Studies - BrdU (5-bromo-2'-deoxyuridine), Thymidine analog (ab142567)Massey, AJ PLoS One. 2015 Jul 28;10(7):e0134306. doi: 10.1371/journal.pone.0134306. eCollection 2015. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Estimation of S-phase Duration using EdU-BrdU Dual Pulse Labelling.

    (From Figure 5A of Massey)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download
  • COA

References (48)

Publishing research using ab142567? Please let us know so that we can cite the reference in this datasheet.

ab142567 has been referenced in 48 publications.

  • Aass KR  et al. Intracellular IL-32 regulates mitochondrial metabolism, proliferation, and differentiation of malignant plasma cells. iScience 25:103605 (2022). PubMed: 35005550
  • Jiang X  et al. Identification and Validation Prognostic Impact of MiRNA-30a-5p in Lung Adenocarcinoma. Front Oncol 12:831997 (2022). PubMed: 35127546
  • Mir BA  et al. MicroRNA-100 Reduced Fetal Bovine Muscle Satellite Cell Myogenesis and Augmented Intramuscular Lipid Deposition by Modulating IGF1R. Cells 11:N/A (2022). PubMed: 35159261
  • Lasagni Vitar R  et al. Substance P/neurokinin-1 receptor pathway blockade ameliorates limbal stem cell deficiency by modulating mTOR pathway and preventing cell senescence. Stem Cell Reports 17:849-863 (2022). PubMed: 35334220
  • da Silva RAG  et al. Enterococcus faecalis alters endo-lysosomal trafficking to replicate and persist within mammalian cells. PLoS Pathog 18:e1010434 (2022). PubMed: 35390107
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

It works very well

Excellent
Abreviews
Abreviews
abreview image
It works very well. The complementary protocol on the Abcam website is straightforward and easy to follow.
I used it at the concentration of 10 micromolar and left for 2 hours in culture.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 27 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES, NOT FOR USE IN HUMANS"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2023 Abcam plc. All rights reserved.