Calcein AM, fluorescent dye for cell viability (ab141420)
Key features and details
- Cell-permeable fluorescent dye for determining cell viability
- CAS Number: 148504-34-1
- Purity: > 96%
- Soluble in DMSO
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
Calcein AM, fluorescent dye for cell viability -
Description
Cell-permeable fluorescent dye for determining cell viability -
Purity
> 96% -
CAS Number
148504-34-1 -
Chemical structure
Properties
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Excitation
495nm -
Emission
515nm -
Chemical name
N,N'-[[3',6'-Bis(acetyloxy)-3-oxospiro[isobenzofuran-1(3H),9'-
[9H]xanthene]-2',7'-diyl]bis(methylene)]bis[N-[2-[(acetyloxy)methoxy]-2-oxoethyl]-glycine 1,1'-bis[(acetyloxy)methyl] ester -
Molecular weight
994.87 -
Molecular formula
C46H46N2O23 -
PubChem identifier
390986 -
Storage instructions
Store at -20°C. It is important to note that this product is reported to be light sensitive. Store In the Dark. Store under desiccating conditions. -
Solubility overview
Soluble in DMSO -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
For more information on AM esters please visit our AM esters FAQ page.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
CC(=O)OCOC(=O)CN(CC1=C(C=C2C(=C1)C3(C4=CC=CC=C4C(=O)O3)C5=CC(=C(C=C5O2)OC(=O)C)CN(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)OC(=O)C)CC(=O)OCOC(=O)C -
Source
Synthetic
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Research areas
Images
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Chemical Structure - Calcein AM, fluorescent dye for cell viability (ab141420)2D chemical structure image of ab141420, Calcein AM, fluorescent dye for cell viability
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (11)
ab141420 has been referenced in 11 publications.
- Song C et al. Generation of individualized immunocompatible endothelial cells from HLA-I-matched human pluripotent stem cells. Stem Cell Res Ther 13:48 (2022). PubMed: 35109922
- Hall AE et al. RNA splicing is a key mediator of tumour cell plasticity and a therapeutic vulnerability in colorectal cancer. Nat Commun 13:2791 (2022). PubMed: 35589755
- Weng X et al. Targeted delivery and ROS-responsive release of Resolvin D1 by platelet chimeric liposome ameliorates myocardial ischemia-reperfusion injury. J Nanobiotechnology 20:454 (2022). PubMed: 36266658
- Derici GE et al. Antidiabetic activities of Bolanthus spergulifolius (Caryophyllaceae) extracts on insulin-resistant 3T3-L1 adipocytes. PLoS One 16:e0252707 (2021). PubMed: 34133443
- Zhang Q et al. Adjustable and ultrafast light-cured hyaluronic acid hydrogel: promoting biocompatibility and cell growth. J Mater Chem B 8:5441-5450 (2020). PubMed: 32555786