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    products/biochemicals/camptothecin-dna-topoisomerase-inhibitor-ab120115.pdf

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Epigenetics and Nuclear Signaling DNA / RNA DNA Synthesis Topoisomerases
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Camptothecin, DNA topoisomerase inhibitor (ab120115)

  • Datasheet
  • SDS
  • COA
Submit a review Submit a question References (2)

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Chemical Structure - Camptothecin, DNA topoisomerase inhibitor (ab120115)
  • Immunocytochemistry/ Immunofluorescence - Camptothecin, DNA topoisomerase inhibitor (ab120115)
  • Functional Studies - Camptothecin, DNA topoisomerase inhibitor (ab120115)
  • Functional Studies - Camptothecin, DNA topoisomerase inhibitor (ab120115)

Key features and details

  • DNA topoisomerase inhibitor
  • CAS Number: 7689-03-4
  • Soluble in DMSO to 5 mM
  • Form / State: Solid
  • Source: Synthetic

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Overview

  • Product name

    Camptothecin, DNA topoisomerase inhibitor
  • Description

    DNA topoisomerase inhibitor
  • Biological description

    Cell-permeable DNA topoisomerase inhibitor. Potent antitumour and antibiotic agent.

  • CAS Number

    7689-03-4
  • Chemical structure

    Chemical Structure

Properties

  • Chemical name

    (S)-4-Ethyl-4-hydroxy-1H-pyrano-[3'4':6,7] indolizino[1,2-b]quinoline-3,14(4H,12H)-dione
  • Molecular weight

    348.35
  • Molecular formula

    C20H16N2O4
  • PubChem identifier

    24360
  • Storage instructions

    Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months.
  • Solubility overview

    Soluble in DMSO to 5 mM
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Toxic, refer to SDS for further information.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILES

    CC[C@]1(O)C=2C=C3c4nc5ccccc5cc4CN3C(=O)C=2COC1=O
  • Source

    Synthetic

  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Synthesis
    • Topoisomerases
    • Cancer
    • Drug resistance
    • Topoisomerases
    • Biochemicals
    • Chemical Type
    • Biochemicals
    • Biochemicals
    • Pharmacology
    • Enzymes
    • Isomerase
    • General
    • Inhibitors

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab120115 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies
Use at an assay dependent concentration.
Notes
Functional Studies
Use at an assay dependent concentration.

Images

  • Chemical Structure - Camptothecin, DNA topoisomerase inhibitor (ab120115)
    Chemical Structure - Camptothecin, DNA topoisomerase inhibitor (ab120115)
    2D chemical structure image of ab120115, Camptothecin, DNA topoisomerase inhibitor
  • Immunocytochemistry/ Immunofluorescence - Camptothecin, DNA topoisomerase inhibitor (ab120115)
    Immunocytochemistry/ Immunofluorescence - Camptothecin, DNA topoisomerase inhibitor (ab120115)

    ab2893 staining γH2AX (phospho S139) in HeLa cells treated with camptothecin (ab120115), by ICC/IF. Increased nuclear expression of γH2AX (phospho S139) correlates with increased concentration of camptothecin, as described in literature.
    The cells were incubated at 37°C for 3h in media containing different concentrations of ab120115 (camptothecin) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab2893 (10 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

  • Functional Studies - Camptothecin, DNA topoisomerase inhibitor (ab120115)
    Functional Studies - Camptothecin, DNA topoisomerase inhibitor (ab120115)

    HeLa cells were incubated at 37°C for 3h with vehicle control (0 µM) and different concentrations of camptothecin (ab120115). Decreased expression of γH2A.X (phospho S139) in HeLa cells correlates with an increase camptothecin concentration, as described in literature.

    Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 20 µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab2893 at 1 µg/ml and ab10475 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.

  • Functional Studies - Camptothecin, DNA topoisomerase inhibitor (ab120115)
    Functional Studies - Camptothecin, DNA topoisomerase inhibitor (ab120115)

    Functional assays: Caspase 3 (active) Red Staining Kit (ab65617)

    Caspase 3 activity in Jurkat cells (3 x10e5 cells) following 24 hour exposure to 2 uM Camptothecin (ab120115) with or without 50 μM caspase inhibitor Z-VAD(OMe)-FMK (ab120487). Background signal subtracted, duplicates; +/- SD.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download
  • COA

References (2)

Publishing research using ab120115? Please let us know so that we can cite the reference in this datasheet.

ab120115 has been referenced in 2 publications.

  • Whelan DR & Rothenberg E Super-resolution mapping of cellular double-strand break resection complexes during homologous recombination. Proc Natl Acad Sci U S A 118:N/A (2021). PubMed: 33707212
  • Whelan DR  et al. Super-resolution visualization of distinct stalled and broken replication fork structures. PLoS Genet 16:e1009256 (2020). PubMed: 33370257

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