CGP 57380, MNK inhibitor (ab120365)
Key features and details
- MNK inhibitor
- CAS Number: 522629-08-9
- Soluble in DMSO to 100 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
CGP 57380, MNK inhibitor -
Description
MNK inhibitor -
Biological description
Low-molecular-weight kinase inhibitor of MAP-kinase interacting kinase-1 (MNK1) and MNK2 (IC50 values are 0.87 and 1.6 μM, respectively). Inhibits a range of other kinases (IC50 values in μM shown in brackets) including: CKI (0.51) Aurora B (2.5) DYRK3 (3.2) SGK1( 2.7) BRSK2 (1.1) and Lck (2.5).
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CAS Number
522629-08-9 -
Chemical structure
Properties
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Chemical name
N3-(4-Fluorophenyl)-1H-pyrazolo-[3,4-d]pyrimidine-3,4-diamine -
Molecular weight
244.23 -
Molecular formula
C11H9FN6 -
PubChem identifier
11644425 -
Storage instructions
Store at +4°C. The product can be stored for up to 12 months. -
Solubility overview
Soluble in DMSO to 100 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
Fc1ccc(cc1)Nc3nnc2ncnc(N)c23 -
Source
Synthetic
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Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab120365 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| Notes |
|---|
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Functional Studies
Use at an assay dependent concentration. |
Images
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Chemical Structure - CGP 57380, MNK inhibitor (ab120365)2D chemical structure image of ab120365, CGP 57380, MNK inhibitor
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Functional Studies - CGP 57380, MNK inhibitor (ab120365)ab76256 staining eIF4E (phospho S209) in serum starved HEK193 cells treated with CGP 57380 (ab120365), by ICC/IF. Decrease in eIF4E (phospho S209) expression correlates with increased concentration of CGP 57380, as described in literature.
The cells were incubated at 37°C for 1h in media containing different concentrations of ab120365 (CGP 57380) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab76256 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (2)
ab120365 has been referenced in 2 publications.
- Moore CE et al. MNK1 and MNK2 mediate adverse effects of high-fat feeding in distinct ways. Sci Rep 6:23476 (2016). PubMed: 27087055
- Leong SY et al. The role of Misshapen NCK-related kinase (MINK), a novel Ste20 family kinase, in the IRES-mediated protein translation of human enterovirus 71. PLoS Pathog 11:e1004686 (2015). PubMed: 25747578