Ciglitazone, PPARgamma agonist (ab141139)
Key features and details
- Selective PPARγ agonist
- CAS Number: 74772-77-3
- Purity: > 98%
- Soluble in DMSO to 100 mM and 1eq. NaOH to 100 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
Ciglitazone, PPARgamma agonist -
Description
Selective PPARγ agonist -
Biological description
Selective PPARγ agonist (EC50 = 3 µM). Shows antidiabetic and antineoplastic activity. Induces cell cycle arrest and apoptosis. Active in vivo.
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Purity
> 98% -
CAS Number
74772-77-3 -
Chemical structure
Properties
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Chemical name
5-[[4-[(1-Methylcyclohexyl)methoxy]phenyl]methyl]-2,4-thiazolidinedione -
Molecular weight
333.44 -
Molecular formula
C18H23NO3S -
PubChem identifier
2750 -
Storage instructions
Store at Room Temperature. The product can be stored for up to 12 months. -
Solubility overview
Soluble in DMSO to 100 mM and 1eq. NaOH to 100 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Toxic, refer to SDS for further information
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
CC1(CCCCC1)COc3ccc(CC2SC(=O)NC2=O)cc3 -
Source
Synthetic
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Research areas
- Signal Transduction
- Signaling Pathway
- Nuclear Signaling
- Nuclear Hormone Receptors
- Retinoic & Retinoid
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Orphan Nuclear Receptors
Associated products
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab141139 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| Notes |
|---|
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Functional Studies
Use at an assay dependent concentration. |
Images
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Chemical Structure - Ciglitazone, PPARgamma agonist (ab141139)2D chemical structure image of ab141139, Ciglitazone, PPARgamma agonist
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Functional Studies - Ciglitazone, PPARgamma agonist (ab141139)ab19481 staining PPARγ in U-87 MG cells treated with ciglitazone (ab141139), by ICC/IF. Increase of PPARγ cytoplasmic expression correlates with increased concentration of ciglitazone, as described in literature.
The cells were incubated at 37°C for 24 hours in media containing different concentrations of ab141139 (ciglitazone) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab19481 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab141139 has not yet been referenced specifically in any publications.