CNQX, AMPA / kainate antagonist (ab120017)
Key features and details
- AMPA / kainate antagonist
- CAS Number: 115066-14-3
- Soluble in DMSO to 100 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
CNQX, AMPA / kainate antagonist -
Description
AMPA / kainate antagonist -
Biological description
Potent, competitive AMPA / kainate receptor antagonist. Also antagonist at NMDA receptor glycine site.
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CAS Number
115066-14-3 -
Chemical structure
Properties
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Chemical name
6-Cyano-7-nitroquinoxaline-2,3-dione -
Molecular weight
232.16 -
Molecular formula
C9H4N4O4 -
PubChem identifier
3721046 -
Storage instructions
Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in DMSO to 100 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
[O-][N+](=O)c1cc2NC(=O)C(=O)Nc2cc1C#N -
Source
Synthetic
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Research areas
Associated products
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab120017 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| Notes |
|---|
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Functional Studies
Use at an assay dependent concentration. |
Images
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Chemical Structure - CNQX, AMPA / kainate antagonist (ab120017)2D chemical structure image of ab120017, CNQX, AMPA / kainate antagonist
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Immunocytochemistry/ Immunofluorescence - CNQX, AMPA / kainate antagonist (ab120017)ab96379 staining MEK1 (phospho S298) in SK-N-SH cells treated with CNQX (ab120017), by ICC/IF. Decrease in MEK1 (phospho S298) expression correlates with increased concentration of CNQX, as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120017 (CNQX) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab96379 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. -
Cellular activation - CNQX, AMPA / kainate antagonist (ab120017)Image from Ildiko P, et al. Plos One, 8(3), e57694. Fig 4a,; doi: 110.1371/journal.pone.0057694Left and Middle: Representative IOS amplitude map and field response curve under control condition and under application of 20 µM CNQX, respectively. The colorbar indicates the maximum change of the transmittance compared to the resting light intensity. A Right: Spatial visualization of the percentage of control changes of IOS signal caused by CNQX application.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (70)
ab120017 has been referenced in 70 publications.
- Kleidonas D & Vlachos A Scavenging Tumor Necrosis Factor a Does Not Affect Inhibition of Dentate Granule Cells Following In Vitro Entorhinal Cortex Lesion. Cells 10:N/A (2021). PubMed: 34831454
- Sanders SS et al. The palmitoyl acyltransferase ZDHHC14 controls Kv1-family potassium channel clustering at the axon initial segment. Elife 9:N/A (2020). PubMed: 33185190
- Mangieri LR et al. A neural basis for antagonistic control of feeding and compulsive behaviors. Nat Commun 9:52 (2018). PubMed: 29302029
- Soh H et al. Deletion of KCNQ2/3 potassium channels from PV+ interneurons leads to homeostatic potentiation of excitatory transmission. Elife 7:N/A (2018). PubMed: 30382937
- Beamer M et al. Primary blast injury causes cognitive impairments and hippocampal circuit alterations. Exp Neurol 283:16-28 (2016). PubMed: 27246999