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CNQX disodium salt, AMPA / kainate antagonist (ab120044)

Submit a review Q&A (3)References (27)
Chemical Structure - CNQX disodium salt, AMPA / kainate antagonist (ab120044)
  • Functional Studies - CNQX disodium salt, AMPA / kainate antagonist (ab120044)

Key features and details

  • AMPA / kainate antagonist; water soluble
  • CAS Number: 479347-85-8
  • Soluble in water to 25 mM
  • Form / State: Solid
  • Source: Synthetic

Overview

  • Product name

    CNQX disodium salt, AMPA / kainate antagonist

  • Description

    AMPA / kainate antagonist; water soluble

  • Biological description

    Water soluble, potent, competitive AMPA / kainate receptor antagonist. Also antagonist at NMDA receptor glycine site.

    Also available in simple stock solutions (ab144488) - add 1 ml of water to get an exact, ready-to-use concentration.

  • CAS Number

    479347-85-8

  • Chemical structure

    Chemical Structure

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab120044 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies
Use at an assay dependent concentration.
Notes
Functional Studies
Use at an assay dependent concentration.

Images

  • Chemical Structure - CNQX disodium salt, AMPA / kainate antagonist (ab120044)
    Chemical Structure - CNQX disodium salt, AMPA / kainate antagonist (ab120044)
    2D chemical structure image of ab120044, CNQX disodium salt, AMPA / kainate antagonist
  • Functional Studies - CNQX disodium salt, AMPA / kainate antagonist (ab120044)
    Functional Studies - CNQX disodium salt, AMPA / kainate antagonist (ab120044)
    ab96379 staining MEK1 (phospho S298) in SK-N-SH cells treated with CNQX disodium salt (ab120044), by ICC/IF. Decrease in MEK1 (phospho S298) expression correlates with increased concentration of CNQX disodium salt, as described in literature.
    The cells were incubated at 37°C for 24h in media containing different concentrations of ab120044 (CNQX disodium salt) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab96379 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (27)

Publishing research using ab120044? Please let us know so that we can cite the reference in this datasheet.

ab120044 has been referenced in 27 publications.

  • Wei XP  et al. A novel reticular node in the brainstem synchronizes neonatal mouse crying with breathing. Neuron 110:644-657.e6 (2022). PubMed: 34998469
  • Zhang J  et al. Severe deficiency of the voltage-gated sodium channel NaV1.2 elevates neuronal excitability in adult mice. Cell Rep 36:109495 (2021). PubMed: 34348148
  • Ivanova D  et al. Control of synaptic vesicle release probability via VAMP4 targeting to endolysosomes. Sci Adv 7:N/A (2021). PubMed: 33931449
  • Vevea JD & Chapman ER Acute disruption of the synaptic vesicle membrane protein synaptotagmin 1 using knockoff in mouse hippocampal neurons. Elife 9:N/A (2020). PubMed: 32515733
  • Battaglia CR  et al. Corticotropin-releasing hormone (CRH) alters mitochondrial morphology and function by activating the NF-kB-DRP1 axis in hippocampal neurons. Cell Death Dis 11:1004 (2020). PubMed: 33230105
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-3 of 3 Abreviews or Q&A

Question

Dear Tech,

I have tried to heat up the solution to 37oC but the chemicals still did not completely dissovle.

Would you please advice what is the optimal temperature for heating up the solution which the chemical would not be degraded?

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Answer

The compound may contain larger granules, which will require more effort to dissolve. We would recommend to use heat and sonication.

· Heat in a 50°C water bath for approximately 5 minutes
· Vortex/sonication on removal
· Repeat as necessary

The above method was successfully used to make a 20 mM solution.

Read More

Question

The label of the vial ab120044 that I received showed that the molecular weight of CNQX disodium salt is 312.14. However, the molecular weight shown on the online datasheet is 276.12. Could you please advice which one is the correct one?

On the other hand, I was trying to dissolve the chemical and found that the chemical could not completely dissolve which a cloudy solution was obtained. Do you have any suggestion to improve this?

Thank you in advance for your help in this matter.

Read More
Answer

The molecular weight and formula published on our website represent the molecular weight and formula of the product as depicted in the chemical structure displayed on our website. However, molecular weights (and formulae) can vary slightly from batch to batch - due to water composition, or due to a change of salt. These changes will be indicated on the Certificate of authenticity that accompanies your product. The changes should not affect the biological activity of your product, but it is important that you take them into account when making up solutions. You should therefore always use the batch molecular weight as stated on the Certificate of authenticity or the vial label.

Regarding the solubility issue, gentle warming is recommended that may be able to help the dissolution.

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Question

Can you provide additional details on the conditions of RP-HPLC used to purify this product?

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Answer

Thank you for contacting Abcam and for your interest in our products.


Regarding ab120044 and ab120169, we don't use HPLC to purify the product, it is used only to analyse it.Our methods of purification are proprietary. We would therefore need more clarification about the experiment you are conducting in order to provide further advice.


Please do not hesitate to contact me if you have any additional questions.

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