CNQX disodium salt, AMPA / kainate antagonist (ab120044)
Key features and details
- AMPA / kainate antagonist; water soluble
- CAS Number: 479347-85-8
- Soluble in water to 25 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
CNQX disodium salt, AMPA / kainate antagonist -
Description
AMPA / kainate antagonist; water soluble -
Biological description
Water soluble, potent, competitive AMPA / kainate receptor antagonist. Also antagonist at NMDA receptor glycine site.
Also available in simple stock solutions (ab144488) - add 1 ml of water to get an exact, ready-to-use concentration. -
CAS Number
479347-85-8 -
Chemical structure
Properties
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Chemical name
1,2,3,4-Tetrahydro-7-nitro-2,3-dioxoquinoxaline-6-carbonitrile disodium -
Molecular weight
276.12 -
Molecular formula
C9H2N4Na2O4 -
PubChem identifier
2821 -
Storage instructions
Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in water to 25 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
[Na+].[Na+].[O-][N+](=O)c1cc2nc([O-])c([O-])nc2cc1C#N -
Source
Synthetic
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Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab120044 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| Notes |
|---|
|
Functional Studies
Use at an assay dependent concentration. |
Images
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Chemical Structure - CNQX disodium salt, AMPA / kainate antagonist (ab120044)2D chemical structure image of ab120044, CNQX disodium salt, AMPA / kainate antagonist
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Functional Studies - CNQX disodium salt, AMPA / kainate antagonist (ab120044)ab96379 staining MEK1 (phospho S298) in SK-N-SH cells treated with CNQX disodium salt (ab120044), by ICC/IF. Decrease in MEK1 (phospho S298) expression correlates with increased concentration of CNQX disodium salt, as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120044 (CNQX disodium salt) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab96379 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (33)
ab120044 has been referenced in 33 publications.
- Wei XP et al. A novel reticular node in the brainstem synchronizes neonatal mouse crying with breathing. Neuron 110:644-657.e6 (2022). PubMed: 34998469
- Bonnycastle K et al. FMRP Sustains Presynaptic Function via Control of Activity-Dependent Bulk Endocytosis. J Neurosci 42:1618-1628 (2022). PubMed: 34996816
- Mishra P & Narayanan R Conjunctive changes in multiple ion channels mediate activity-dependent intrinsic plasticity in hippocampal granule cells. iScience 25:103922 (2022). PubMed: 35252816
- Špirková A et al. Glutamate can act as a signaling molecule in mouse preimplantation embryos†. Biol Reprod 107:916-927 (2022). PubMed: 35746896
- Koba S et al. A brainstem monosynaptic excitatory pathway that drives locomotor activities and sympathetic cardiovascular responses. Nat Commun 13:5079 (2022). PubMed: 36038592