Curcumin (Diferuloylmethane), Lipooxygenase inhibitor (ab120618)
Key features and details
- Flavonoid. Lipooxygenase inhibitor. Antioxidant, anti-inflammatory agent.
- CAS Number: 458-37-7
- Soluble in ethanol to 10 mM and in DMSO to 10 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
Curcumin (Diferuloylmethane), Lipooxygenase inhibitor -
Description
Flavonoid. Lipooxygenase inhibitor. Antioxidant, anti-inflammatory agent. -
Alternative names
- Diferuloylmethane
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Biological description
Flavonoid derived from Curcuma longa plant. Potent antioxidant, anti-inflammatory, analgesic and anticancer agent. Lipooxygenase and cyclooxygenase inhibitor (IC50 values are 8 and 52 μM, respectively). Blood-brain barrier permeable. Shows diverse range of actions including free radical scavenging activity in vitro and in vivo, cardio and neuroprotective effects.
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CAS Number
458-37-7 -
Chemical structure
Properties
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Chemical name
(1E,6E)-1,7-Bis(4-hydroxy-3-methoxyphenyl)-1,6-heptadiene-3,5-dione -
Molecular weight
368.38 -
Molecular formula
C21H20O6 -
PubChem identifier
969516 -
Storage instructions
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in ethanol to 10 mM and in DMSO to 10 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
COC1=C(C=CC(=C1)C=CC(=O)CC(=O)C=CC2=CC(=C(C=C2)O)OC)O -
Source
Synthetic
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Research areas
- Signal Transduction
- Signaling Pathway
- Nuclear Signaling
- Nuclear Hormone Receptors
- Retinoic & Retinoid
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Co-activators/co-repressors
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Orphan Nuclear Receptors
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Nucleotide metabolism
- Molecular processes
- Mitochondrial transcription
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipases
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab120618 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| Notes |
|---|
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Functional Studies
Use at an assay dependent concentration. |
Images
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Chemical Structure - Curcumin (Diferuloylmethane), Lipooxygenase inhibitor (ab120618)2D chemical structure image of ab120618, Curcumin (Diferuloylmethane), Lipooxygenase inhibitor
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Immunocytochemistry/ Immunofluorescence - Curcumin (Diferuloylmethane), Lipooxygenase inhibitor (ab120618)ab32137 staining c-Jun in HeLa cells treated with curcumin (diferuloylmethane) (ab120618), by ICC/IF. Decrease in c-Jun expression correlates with increased concentration of curcumin (diferuloylmethane) as described in literature.
The cells were incubated at 37°C for 4h in media containing different concentrations of ab120618 (curcumin (diferuloylmethane)) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab32137 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (1)
ab120618 has been referenced in 1 publication.
- Dao TT et al. Demethoxycurcumin Is A Potent Inhibitor of P-Type ATPases from Diverse Kingdoms of Life. PLoS One 11:e0163260 (2016). PubMed: 27644036