Cycloheximide, Protein synthesis inhibitor (ab120093)
Key features and details
- Protein synthesis inhibitor
- CAS Number: 66-81-9
- Soluble in water to 25 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
Cycloheximide, Protein synthesis inhibitor -
Description
Protein synthesis inhibitor -
Biological description
Protein synthesis inhibitor. Antifungal antibiotic.
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CAS Number
66-81-9 -
Chemical structure
Properties
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Chemical name
3-[2-(3,5-Dimethyl-2-oxocyclohexyl)-2-hydroxyethyl]glutarimide -
Molecular weight
281.35 -
Molecular formula
C15H23NO4 -
PubChem identifier
6197 -
Storage instructions
Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in water to 25 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Toxic, refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
O=C2CC(C[C@@H](O)[C@@H]1C[C@@H](C)C[C@H](C)C1=O)CC(=O)N2 -
Source
Synthetic
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Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab120093 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| Notes |
|---|
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Functional Studies
Use at an assay dependent concentration. |
Images
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Chemical Structure - Cycloheximide, Protein synthesis inhibitor (ab120093)2D chemical structure image of ab120093, Cycloheximide, Protein synthesis inhibitor
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Functional Studies - Cycloheximide, Protein synthesis inhibitor (ab120093)Functional assays: Caspase 8 (active) FITC Staining Kit (ab65614)
Active caspase 8 in control Jurkat cells (10e6/mL) or cells treated for five hours with 10 ug/mL Cyclohexamide (CHX) (ab120093) or four hours with 25 ug/mL Mitomycin C (MitoC) (ab120797). Background signal subtracted, duplicates; +/- SD.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (22)
ab120093 has been referenced in 22 publications.
- Xu S et al. MicroRNA-200c-targeted contactin 1 facilitates the replication of influenza A virus by accelerating the degradation of MAVS. PLoS Pathog 18:e1010299 (2022). PubMed: 35171955
- Chu Y et al. LUBAC and OTULIN regulate autophagy initiation and maturation by mediating the linear ubiquitination and the stabilization of ATG13. Autophagy 17:1684-1699 (2021). PubMed: 32543267
- Xu C et al. ATE1 Inhibits Liver Cancer Progression through RGS5-Mediated Suppression of Wnt/ß-Catenin Signaling. Mol Cancer Res 19:1441-1453 (2021). PubMed: 34158395
- Gao G et al. TGF-ß1 Facilitates TAp63a Protein Lysosomal Degradation to Promote Pancreatic Cancer Cell Migration. Biology (Basel) 10:N/A (2021). PubMed: 34203341
- Li W et al. SARS-CoV-2 Nsp5 Activates NF-?B Pathway by Upregulating SUMOylation of MAVS. Front Immunol 12:750969 (2021). PubMed: 34858407