D-AP5, NMDA glutamate site antagonist (ab120003)
Key features and details
- NMDA glutamate site antagonist
- CAS Number: 79055-68-8
- Soluble in water to 100 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
D-AP5, NMDA glutamate site antagonist -
Description
NMDA glutamate site antagonist -
Alternative names
- APV
- D-APV
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Biological description
Competitive NMDA receptor glutamate site antagonist. More active form of DL-AP5.
Also available in simple stock solutions (ab144482) - add 1 ml of water to get an exact, ready-to-use concentration.
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CAS Number
79055-68-8 -
Chemical structure
Properties
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Chemical name
D-(-)-2-Amino-5-phosphonopentanoic acid -
Molecular weight
197.13 -
Molecular formula
C5H12NO5P -
PubChem identifier
135342 -
Storage instructions
Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in water to 100 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
N[C@H](CCCP(=O)(O)O)C(=O)O -
Source
Synthetic
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Research areas
Images
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Chemical Structure - D-AP5, NMDA glutamate site antagonist (ab120003)2D chemical structure image of ab120003, D-AP5, NMDA glutamate site antagonist
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Cellular activation - D-AP5, NMDA glutamate site antagonist (ab120003)Image from Barker M et al., Plos One, 7(5), e35955. Fig 1,; doi: 10.1371/journal.pone.0035955(A) Photomicrograph of a DCN fusiform cell filled with lucifer yellow (top) and whole cell voltage clamp recording of this fusiform cell while stimulating the LVN (bottom). (B) Photomicrograph of a DCN granule cell filled with lucifer yellow (top) and whole cell voltage clamp recording of this granule cell while stimulating the LVN (bottom). Both cells were held at -68 mV and the LVN was stimulated at 0.3 Hz. Glutamatergic EPSCs are represented in black and are blocked by 50 µm D-AP5 and 10 µm NBQX (traces in red). Each trace represents an average of 10-20 single traces. The arrowhead represents the artifact of stimulus that has been removed for clarity. Scale bar: (A) 50 µm, (B) 20 µm.
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Functional Studies - D-AP5, NMDA glutamate site antagonist (ab120003)Image from Herman MA et al., PLoS One. 2011;6(11):e26501. Fig 2(A).; doi: 10.1371/journal.pone.0026501. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Averaged Ca2+ transients (500 Hz line scans) evoked by 40 ms voltage step in a dendrite (left) and spine (right) in control (black), D-AP5 (red, 10 µM), after a 10 min washout of D-AP5 (green), and in 5 µM NMDA (blue). Mibefradil (20 µM), nimodipine (20 µM) and TTX (0.5 µM) were present throughout. -
Functional Studies - D-AP5, NMDA glutamate site antagonist (ab120003)Image from Brady JD et al., Neuroscience. 2010;168(1):108-17. Fig 3.; doi: 10.1016/j.neuroscience.2010.03.009 with permission from Elsevier.Representative voltage-clamp recording (Vh= −60 mV, ECl−=+8 mV) of a Purkinje cells response to simulated ischemia and sequential block of glutamate receptors and GABAA receptors.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (246)
ab120003 has been referenced in 246 publications.
- Prakash M et al. Selective control of synaptically-connected circuit elements by all-optical synapses. Commun Biol 5:33 (2022). PubMed: 35017641
- Vierock J et al. BiPOLES is an optogenetic tool developed for bidirectional dual-color control of neurons. Nat Commun 12:4527 (2021). PubMed: 34312384
- Pan Y et al. Neuronal activity recruits the CRTC1/CREB axis to drive transcription-dependent autophagy for maintaining late-phase LTD. Cell Rep 36:109398 (2021). PubMed: 34289350
- Li L et al. a2d-1 switches the phenotype of synaptic AMPA receptors by physically disrupting heteromeric subunit assembly. Cell Rep 36:109396 (2021). PubMed: 34289359
- Zhang J et al. Severe deficiency of the voltage-gated sodium channel NaV1.2 elevates neuronal excitability in adult mice. Cell Rep 36:109495 (2021). PubMed: 34348148