Deferoxamine mesylate, Iron chelator (ab120727)
Key features and details
- Iron chelator
- CAS Number: 138-14-7
- Soluble in water to 100 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
Deferoxamine mesylate, Iron chelator -
Description
Iron chelator -
Alternative names
- desferal
- desferrioxamine B
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Biological description
Iron chelator. Blood-brain barrier permeable. Displays a number of biological actions such as immunomodulation, inhibition of inflammation and atherosclerosis, and reduction of neuronal death.
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CAS Number
138-14-7 -
Chemical structure
Properties
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Chemical name
N4-[5-[[4-5-(Acetylhydroxyamino)pentyl]-1,4-dioxobutyl]hydroxyaminopentyl-N1-(5-aminopentyl)-N1-hydroxybutanediamide methanesulfonate -
Molecular weight
656.79 -
Molecular formula
C25H48N6O8.CH3SO3H -
PubChem identifier
62881 -
Storage instructions
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in water to 100 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
CC(=O)N(CCCCCNC(=O)CCC(=O)N(CCCCCNC(=O)CCC(=O)N(CCCCCN)O)O)O.CS(=O)(=O)O -
Source
Synthetic
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Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab120727 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| Notes |
|---|
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Functional Studies
Use at an assay dependent concentration. |
Images
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Chemical Structure - Deferoxamine mesylate, Iron chelator (ab120727)2D chemical structure image of ab120727, Deferoxamine mesylate, Iron chelator
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Functional Studies - Deferoxamine mesylate, Iron chelator (ab120727)Titration of HeLa-DFO extract within the working range of the assay. Background subtracted data from duplicate measurements are plotted. To induce HIF1 alpha protein levels, HeLa cells were treated with 500 µM DFO (ab120727) for 24 hours. -
Deferoxamine mesylate, Iron chelator (ab120727)Dose-dependent induction of HIF1 alpha in HeLa cells by DFO (ab120727). HeLa cells were cultured in 96-well tissue culture plates and were either untreated or exposed to varying dose of DFO for 24 hours. Raw data with standard deviation is plotted from triplicate measurements.
Dose-dependent induction of HIF1 alpha in HeLa cells by DFO (ab120727). HeLa cells were cultured in 96-well tissue culture plates and were either untreated or exposed to varying dose of DFO for 24 hours. Raw data with standard deviation is plotted from triplicate measurements. -
Deferoxamine mesylate, Iron chelator (ab120727)Comparison of HIF1 alpha expression in HeLa cell extracts (with and without DFO treatment) by SimpleStep ELISA (barchart) and western blot (top). Background subtracted OD450 nm data from three loading concentrations are shown. The HIF1 alpha detector antibody was used to blot the same lysates as analyzed by SimpleStep ELISA (40 µg loaded/lane). The GAPDH blot is included to show the relative loads of each lysate. In the HeLa cell line, DFO treatment is required to detect HIF1 alpha protein by both SimpleStep ELISA and western blot.
Comparison of HIF1 alpha expression in HeLa cell extracts (with and without DFO treatment) by SimpleStep ELISA (barchart) and western blot (top). Background subtracted OD450 nm data from three loading concentrations are shown. The HIF1 alpha detector antibody was used to blot the same lysates as analyzed by SimpleStep ELISA (40 µg loaded/lane). The GAPDH blot is included to show the relative loads of each lysate. In the HeLa cell line, DFO treatment is required to detect HIF1 alpha protein by both SimpleStep ELISA and western blot.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (9)
ab120727 has been referenced in 9 publications.
- You JH et al. PGRMC1-dependent lipophagy promotes ferroptosis in paclitaxel-tolerant persister cancer cells. J Exp Clin Cancer Res 40:350 (2021). PubMed: 34749765
- Tadokoro T et al. Mitochondria-dependent ferroptosis plays a pivotal role in doxorubicin cardiotoxicity. JCI Insight 5:N/A (2020). PubMed: 32376803
- Rakshit J et al. Iron chelator Deferoxamine protects human neuroblastoma cell line SH-SY5Y from 6-Hydroxydopamine-induced apoptosis and autophagy dysfunction. J Trace Elem Med Biol 57:126406 (2020). PubMed: 31570251
- Sugiyama A et al. xCT inhibitor sulfasalazine depletes paclitaxel-resistant tumor cells through ferroptosis in uterine serous carcinoma. Oncol Lett 20:2689-2700 (2020). PubMed: 32782585
- Fu C et al. The transcription factor ZFHX3 is crucial for the angiogenic function of hypoxia-inducible factor 1a in liver cancer cells. J Biol Chem 295:7060-7074 (2020). PubMed: 32277050
- Nishimura K et al. Type 3 iodothyronine deiodinase is expressed in human induced pluripotent stem cell derived cardiomyocytes. Life Sci 203:276-281 (2018). PubMed: 29684447
- Yao Q et al. Mesoporous silicate nanoparticles/3D nanofibrous scaffold-mediated dual-drug delivery for bone tissue engineering. J Control Release 279:69-78 (2018). PubMed: 29649529
- Furukawa T et al. Chronic diazepam administration increases the expression of Lcn2 in the CNS. Pharmacol Res Perspect 5:e00283 (2017). PubMed: 28596835
- Sias SR & Ingraham JL Isolation and analysis of mutants of Pseudomonas aeruginosa unable to assimilate nitrate. Arch Microbiol 122:263-70 (1979). PubMed: 120727