Doxorubicin hydrochloride, Topoisomerase II inhibitor (ab120629)
Key features and details
- Topoisomerase II inhibitor. Antibiotic with cytotoxic actions.
- CAS Number: 25316-40-9
- Purity: > 98%
- Soluble in DMSO to 25 mM and in water to 50 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
Doxorubicin hydrochloride, Topoisomerase II inhibitor -
Description
Topoisomerase II inhibitor. Antibiotic with cytotoxic actions. -
Alternative names
- Adriamycin
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Purity
> 98% -
CAS Number
25316-40-9 -
Chemical structure
Properties
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Chemical name
(8S,10S)-10-[(3-Amino-2,3,6-trideoxy-L-lyxohexopyranosyl)oxy]-7,8,9,10-tetrahydro-6,8,11-trihydroxy-8-(hydroxyacetyl)-1-methoxynaphthacene-5,12-dione hydrochloride -
Molecular weight
579.98 -
Molecular formula
C27H29NO11.HCl -
PubChem identifier
443939 -
Storage instructions
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in DMSO to 25 mM and in water to 50 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Toxic, refer to SDS for further information
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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Source
Synthetic
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Research areas
Images
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Chemical Structure - Doxorubicin hydrochloride, Topoisomerase II inhibitor (ab120629)2D chemical structure image of ab120629, Doxorubicin hydrochloride, Topoisomerase II inhibitor
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Western blot - Doxorubicin hydrochloride, Topoisomerase II inhibitor (ab120629)All lanes : Anti-DR5 antibody [EPR22276] (ab230969) at 1/1000 dilution
Lane 1 : Untreated HCT116 (human colorectal carcinoma epithelial cell), whole cell lysate
Lane 2 : HCT116 treated with 0.5 µM doxorubicin (ab120629) for 24 hours, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Exposure time: 3 minutesThe molecular weight observed is consistent with what has been described in the literature, the 40kDa band is a cleaved form (PMID: 20515924, 16297203).
Blocking/Dilution buffer: 5% NFDM/TBST.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (9)
ab120629 has been referenced in 9 publications.
- Machino H et al. The metabolic stress-activated checkpoint LKB1-MARK3 axis acts as a tumor suppressor in high-grade serous ovarian carcinoma. Commun Biol 5:39 (2022). PubMed: 35017636
- Tai YK et al. Modulated TRPC1 Expression Predicts Sensitivity of Breast Cancer to Doxorubicin and Magnetic Field Therapy: Segue Towards a Precision Medicine Approach. Front Oncol 11:783803 (2021). PubMed: 35141145
- Liu M et al. Identification of a prognostic chemoresistance-related gene signature associated with immune microenvironment in breast cancer. Bioengineered 12:8419-8434 (2021). PubMed: 34661511
- Younis NS Doxorubicin-Induced Cardiac Abnormalities in Rats: Attenuation via Sandalwood Oil. Pharmacology 105:522-530 (2020). PubMed: 31747659
- Thakur A et al. Inhibition of Glioma Cells' Proliferation by Doxorubicin-Loaded Exosomes via Microfluidics. Int J Nanomedicine 15:8331-8343 (2020). PubMed: 33149579
- Martins CA et al. Pomolic acid exhibits anticancer potential against a docetaxel-resistant PC3 prostate cell line. Oncol Rep 42:328-338 (2019). PubMed: 31002376
- Kalenderoglou N et al. Cannabidiol Reduces Leukemic Cell Size - But Is It Important? Front Pharmacol 8:144 (2017). PubMed: 28392768
- Zhang D et al. Down-regulation of CHERP inhibits neuroblastoma cell proliferation and induces apoptosis through ER stress induction. Oncotarget 8:80956-80970 (2017). PubMed: 29113358
- Choi J et al. The effect of doxorubicin on MEK-ERK signaling predicts its efficacy in HCC. J Surg Res 150:219-26 (2008). PubMed: 18468633