Dynasore, dynamin inhibitor (ab120192)
Key features and details
- Cell-permeable dynamin inhibitor
- CAS Number: 304448-55-3
- Soluble in DMSO to 100 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
Dynasore, dynamin inhibitor -
Description
Cell-permeable dynamin inhibitor -
Biological description
Cell-permeable dynamin inhibitor, that blocks endocytosis. Non-competitively inhibits dynamin1, dynamin2 and mitochondrial dynamin GTPase activity. Selective over other small GTPases.
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CAS Number
304448-55-3 -
Chemical structure
Properties
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Chemical name
3-Hydroxynaphthalene-2-carboxylic acid (3,4-dihydroxybenzylidene)hydrazide -
Molecular weight
322.31 -
Molecular formula
C18H14N2O4 -
PubChem identifier
135533054 -
Storage instructions
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in DMSO to 100 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
Oc3ccc(/C=N/NC(=O)c1cc2ccccc2cc1O)cc3O -
Source
Synthetic
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Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab120192 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| Notes |
|---|
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Functional Studies
Use at an assay dependent concentration. |
Images
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Chemical Structure - Dynasore, dynamin inhibitor (ab120192)2D chemical structure image of ab120192, Dynasore, dynamin inhibitor
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Immunocytochemistry/ Immunofluorescence - Dynasore, dynamin inhibitor (ab120192)ab66705 staining PAI1 in HeLa cells treated with dynasore (ab120192), by ICC/IF. Increase in PAI1 expression correlates with increased concentration of dynasore, as described in literature.
The cells were incubated at 37°C for 6h in media containing different concentrations of ab120689 (dynasore) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab66705 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue. -
Western blot - Dynasore, dynamin inhibitor (ab120192)Jens H et al. PLoS One. 2016; 11(10): e0164768. doi: 10.1371/journal.pone.0164768 Reproduced under the Creative Commons license https://creativecommons.org/publicdomain/zero/1.0/Western blots of indicated proteins after knockdown with gene-specific siRNA against AP2M1 (ab120192) or non-targeting siRNA transfection control (NT).
siRNAs were transfected into HMVEC-L at the concentration of 100 nM for 48h. The cells were then infected with ANDV (MOI = 0.5) for 24 h or 48 h. Western blots were performed post infection to ensure knockdown of the specific protein expression. The blots were also probed with β-actin specific antibody as the gel-loading control.
Jens H et al. PLoS One. 2016; 11(10): e0164768. doi: 10.1371/journal.pone.0164768
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (48)
ab120192 has been referenced in 48 publications.
- Chen Y et al. Amino acid starvation-induced LDLR trafficking accelerates lipoprotein endocytosis and LDL clearance. EMBO Rep 23:e53373 (2022). PubMed: 34994492
- Belaidi AA et al. Apolipoprotein E potently inhibits ferroptosis by blocking ferritinophagy. Mol Psychiatry N/A:N/A (2022). PubMed: 35484240
- Tanaka H et al. Aberrant autophagy in lysosomal storage disorders marked by a lysosomal SNARE protein shortage due to suppression of endocytosis. J Inherit Metab Dis 45:1191-1202 (2022). PubMed: 36102069
- Watson J et al. Spatially resolved phosphoproteomics reveals fibroblast growth factor receptor recycling-driven regulation of autophagy and survival. Nat Commun 13:6589 (2022). PubMed: 36329028
- Lynn KS et al. Asymmetric distribution of dynamin-2 and ß-catenin relative to tight junction spikes in alveolar epithelial cells. Tissue Barriers N/A:1929786 (2021). PubMed: 34107845
