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  1. Link

    products/biochemicals/dyngo-4a-novel-highly-potentdynamin-inhibitor-ab120689.pdf

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Biochemicals Chemical Type Biochemicals
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Dyngo® 4a, Novel, highly potent dynamin inhibitor (ab120689)

  • Datasheet
  • SDS
  • COA
Submit a review Q&A (4)References (61)

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Chemical Structure - Dyngo<sup>&reg;</sup> 4a, Novel, highly potent&nbsp;dynamin inhibitor (ab120689)
  • Functional Studies - Dyngo<sup>&reg;</sup> 4a, Novel, highly potent&nbsp;dynamin inhibitor (ab120689)
  • Immunocytochemistry/ Immunofluorescence - Dyngo® 4a, Novel, highly potent dynamin inhibitor (ab120689)

Key features and details

  • Novel, highly potent dynamin inhibitor. Dynasore (ab120192) analog.
  • CAS Number: 1256493-34-1
  • Soluble in DMSO to 100 mM

  • Form / State: Solid
  • Source: Synthetic

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Pitstop® 2, Novel cell-permeable clathrin inhibitor (ab120687)

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Overview

  • Product name

    Dyngo® 4a, Novel, highly potent dynamin inhibitor
  • Description

    Novel, highly potent dynamin inhibitor. Dynasore (ab120192) analog.
  • Biological description

    Novel, highly potent dynamin inhibitor (IC50 values are 400 nM and 200 nM at dynamin I and II human-recombinant, respectively). Inhibits clathrin-mediated endocytosis (IC50 = 5.5 µM). Dynasore (ab120192) analog. Prevents botulin neurotoxin A (BoNTs) uptake and internalization, and prevents SNAP25 cleavage. Inhibits BoNTs induced paralysis and delays botulism onset.

  • General notes

    Sold under exclusive licence from Children's Medical Research Institute and Newcastle Innovation Ltd. Dyngo® is a trademark of Children's Medical Research Institute and Newcastle Innovation Ltd.

  • CAS Number

    1256493-34-1
  • Chemical structure

    Chemical Structure

Properties

  • Chemical name

    3-Hydroxy-N'-[(2,4,5-trihydroxyphenyl)methylidene]naphthalene-2-carbohydrazide

  • Molecular weight

    338.31
  • Molecular formula

    C18H14N2O5
  • PubChem identifier

    136227923
  • Storage instructions

    Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months.
  • Solubility overview

    Soluble in DMSO to 100 mM

  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Toxic, refer to SDS for further information.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILES

    C1=CC=C2C=C(C(=CC2=C1)C(=O)N/N=C/C3=CC(=C(C=C3O)O)O)O
  • Source

    Synthetic

  • Research areas

    • Biochemicals
    • Chemical Type
    • Biochemicals
    • Biochemicals
    • Pharmacology
    • Enzymes
    • ATPase & GTPase
    • Inhibitors
    • Biochemicals
    • Pharmacology
    • Signaling
    • Endocytosis & exocytosis
    • Dynamin
    • Biochemicals
    • Research Area
    • Heart disease
    • Signaling
    • Endocytosis & exocytosis
    • Dynamin
    • Biochemicals
    • Research Area
    • Pain & inflammation
    • Signaling
    • Endocytosis & exocytosis
    • Dynamin
    • Biochemicals
    • Research Area
    • Alzheimer's Disease
    • Signaling
    • Endocytosis & exocytosis
    • Dynamin
    • Biochemicals
    • Research Area
    • Diabetes
    • Signaling
    • Endocytosis & exocytosis
    • Dynamin
    • Biochemicals
    • Research Area
    • Hypertension
    • Signaling
    • Endocytosis & exocytosis
    • Dynamin
    • Biochemicals
    • Research Area
    • Obesity
    • Signaling
    • Endocytosis & exocytosis
    • Dynamin
    • Biochemicals
    • Research Area
    • Respiratory disease
    • Signaling
    • Endocytosis & exocytosis
    • Dynamin
    • Biochemicals
    • Research Area
    • Stroke
    • Signaling
    • Endocytosis & exocytosis
    • Dynamin

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab120689 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies
Use at an assay dependent concentration.
Notes
Functional Studies
Use at an assay dependent concentration.

Images

  • Chemical Structure - Dyngo<sup>&reg;</sup> 4a, Novel, highly potent&nbsp;dynamin inhibitor (ab120689)
    Chemical Structure - Dyngo® 4a, Novel, highly potent dynamin inhibitor (ab120689)
    2D chemical structure image of ab120689, Dyngo® 4a, Novel, highly potentdynamin inhibitor
  • Functional Studies - Dyngo<sup>&reg;</sup> 4a, Novel, highly potent&nbsp;dynamin inhibitor (ab120689)
    Functional Studies - Dyngo® 4a, Novel, highly potent dynamin inhibitor (ab120689)Kockx M et al. PLoS One. 2014; 9(10): e111186. doi: 10.1371/journal.pone.0111186 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Primary human monocyte-derived macrophages were treated without (Ctrl) and with ab120689 (Dyngo 4A) for 2 h. Effects on α-tubulin and acetylated tubulin (Ac-tubulin) were determined by confocal microscopy.

     

    Credit: Kockx M et al. PLoS One. 2014; 9(10): e111186. doi: 10.1371/journal.pone.0111186

  • Immunocytochemistry/ Immunofluorescence - Dyngo® 4a, Novel, highly potent dynamin inhibitor (ab120689)
    Immunocytochemistry/ Immunofluorescence - Dyngo® 4a, Novel, highly potent dynamin inhibitor (ab120689)

    ab66705 staining PAI1 in HeLa cells treated with dyngo-4a™(ab120689), by ICC/IF. Increase in PAI1 expression correlates with increased concentration of dyngo-4a™, as described in literature.
    The cells were incubated at 37°C for 6h in media containing different concentrations of ab120689 (dyngo-4a™) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab66705 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download
  • COA

References (61)

Publishing research using ab120689? Please let us know so that we can cite the reference in this datasheet.

ab120689 has been referenced in 61 publications.

  • Cappelli J  et al. Glycine-induced NMDA receptor internalization provides neuroprotection and preserves vasculature following ischemic stroke. iScience 25:103539 (2022). PubMed: 34977503
  • Alyenbaawi H  et al. Seizures are a druggable mechanistic link between TBI and subsequent tauopathy. Elife 10:N/A (2021). PubMed: 33527898
  • Tsvetanova NG  et al. Endosomal cAMP production broadly impacts the cellular phosphoproteome. J Biol Chem 297:100907 (2021). PubMed: 34166681
  • Peng GE  et al. Spatial decoding of endosomal cAMP signals by a metastable cytoplasmic PKA network. Nat Chem Biol 17:558-566 (2021). PubMed: 33649598
  • Batta G  et al. Statin-boosted cellular uptake and endosomal escape of penetratin due to reduced membrane dipole potential. Br J Pharmacol 178:3667-3681 (2021). PubMed: 33908640
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
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1-4 of 4 Abreviews or Q&A

Question


Inquiry: Hi, I was wondering if Dyngo 4a is a reversible inhibitor of dynamin (another inhibitor you sell, Pitstop 2, is reversible upon washing). Also, does serum in the medium inhibit the effects of Dyngo?

Read More

Abcam community

Verified customer

Asked on Jan 25 2013

Answer

Thank you for contacting us.

Unfortunately we do not test the biological activity of our compounds in-house but I have consulted the literature for these types of questions. So far there have been 3 publications using this product:



http://www.ncbi.nlm.nih.gov/pubmed/21832053

http://www.ncbi.nlm.nih.gov/pubmed/22629340

http://www.ncbi.nlm.nih.gov/pubmed/22977254



Unfortunately none of them describe a reversible nature of the Dyngo-4a so we cannot provide this information. The middle one used Ringer's solution which does not contain serum. The first seem to have used media with 0.1% BSA which is quite low levels of serum but they did show functionality with the product.

I wish I could provide more information in this case, please let me know if you have any further questions.

Read More

Abcam Scientific Support

Answered on Jan 25 2013

Question

Dear Sir/Madam

I have purchased dynasore and dyngo 4a from you along with the dynamin toolkit containing iminodyn, dynole and MAB series compounds. The dynamin tool kit compounds contain infomation regarding dynamin binding sites but this information is not available for dynasore nor dyngo. I was wondering if you had any idea about the binding sites in dynamin 2 for these compounds or know of someone who may have an idea?

Kind Regards

Read More

Abcam community

Verified customer

Asked on Jun 28 2012

Answer

Thank you for your enquiry.

Please find information about the biological activity of these compounds below. We also have specific references listed on the datasheet that will provide more information. Reviewing the online literature I have also found additional information.

Dynasore ab120192
Cell permeable dynamin inhibitor, that blocks endocytosis. Non-competitively inhibits dynamin1, dynamin2 and mitochondrial dynamin GTPase activity. Selective over other small GTPases.

https://www.abcam.com/Dynasore-ab120192-references.html

Dynasore, a Dynamin Inhibitor, Inhibits Trypanosoma cruzi Entry into Peritoneal Macrophages

http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0007764


Dyngo-4a ab120689
Novel, highly potent dynamin inhibitor (IC50 values are 380 nM and 2.6 µM at dynamin I and II respectively). Dynasore (ab120192) analogue. Prevents botulin neurotoxin A (BoNTs) uptake and internalisation, and prevents SNAP25 cleavage. Inhibits BoNTs induced paralysis and delays botulism onset.

https://www.abcam.com/Dyngo-4a™-ab120689-references.html

Ascent Scientific Launch Novel Dynamin Inhibitor Dyngo-4a™ with Children's Medical Research Institute and University of Newcastle

http://www.ascentscientific.com/NewsItem.php?article=article011111


I would suggest browsing other online resources that may have additional specific information. I hope this information helps.

Read More

Abcam Scientific Support

Answered on Jun 28 2012

Question

On the Dynamin inhibitors summary table (http://www.ascentscientific.com/Signalling/Endocytosis-&-exocytosis/Dynamin/c/1136), the IC50 of Dyngo-4a for Receptor Mediated Endocytosis Inhibition is >300 µm.
In some publications a total inhibition is observed for ˜30 µm. Is it a typo in the table? Should it be ">300 nm" and not ">300 µm"?

Read More

Abcam community

Verified customer

Asked on Mar 20 2012

Answer

Merci de nous avoir contactés.

Mes collègues d'Ascent m'ont informé qu'une erreur s'est glissée dans le tableau que vous avez mentionné. La colonne 'receptor mediated endocytosis' ne devrait pas comporter de valeur pour l'IC50 de Dyngo-4a car à leur connaissance cette information n'est pas connue.

Ascent Scientificétant maintenantAbcam Biochemicals, la mise à jour des produits et deleurs informations relativesest désormaisdisponible via le lien suivant : https://www.abcam.com/index.html?pageconfig=biochemicals.Une version plus récentedu tableau que vous avez mentionnéest disponibleà la pagehttps://www.abcam.com/index.html?pageconfig=resource&rid=14857.

J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

Read More

Abcam Scientific Support

Answered on Mar 20 2012

Question

Phone call requesting advice on the solubility of Dyngo-4a. Have made a stock solution of 30 mM which has then been diluted to 1/1000 but the compound is precipitating out.

Read More

Abcam community

Verified customer

Asked on Feb 02 2012

Answer

Thank you for contacting us.

Our chemistry team have previously investigated the solubility of Dyngo-4a™. They found that it is soluble to 100 mM in DMSO. They also found that it was possible to achieve a concentration of 0.03 mM in 1 % DMSO/water without precipitation occurring.

The dilution which you are currently performing (30 mM stock diluted by 1/1000) results in a final concentration of 0.03 mM in 0.1 % DMSO/water. Therefore in order to improve the solubility you have been observing I would suggest increasing the DMSO content by a factor of 10.

I hope that this information is helpful. If you would like to discuss this matter further I would be happy to help.

Read More

Abcam Scientific Support

Answered on Feb 02 2012

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