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    products/biochemicals/dynole-31-2-negative-control-for-dynolereg-34-2-ab120464.pdf

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Biochemicals Chemical Type Biochemicals
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Dynole® 31-2, Negative control for Dynolereg 34-2 (ab120464)

  • Datasheet
  • SDS
  • COA
Submit a review Submit a question References (7)

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Chemical Structure - Dynole<sup>&reg;</sup> 31-2, Negative control for Dynole<sup>reg</sup> 34-2 (ab120464)
  • Immunocytochemistry/ Immunofluorescence - Dynole® 31-2, Negative control for Dynolereg 34-2 (ab120464)
  • Functional Studies - Dynole<sup>&reg;</sup> 31-2, Negative control for Dynole<sup>reg</sup> 34-2 (ab120464)

Key features and details

  • Negative control for Dynole® 34-2
  • Soluble in DMSO to 100 mM
  • Form / State: Solid
  • Source: Synthetic

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Overview

  • Product name

    Dynole® 31-2, Negative control for Dynolereg 34-2
  • Description

    Negative control for Dynole® 34-2
  • Biological description

    Negative control molecule for use with Dynole® 34-2 ab120463. Displays no significant activity at dynamin I or II (up to 300 µM).

  • General notes

    Sold under exclusive licence from Children's Medical Research Institute and Newcastle Innovation Ltd. Dynole® is a trademark of Children's Medical Research Institute and Newcastle Innovation Ltd.
  • Chemical structure

    Chemical Structure

Properties

  • Chemical name

    2-Cyano-N-propyl-3-[1-(3-dimethylaminopropyl)-1H-indol-3-yl]acrylamide
  • Molecular weight

    338.45
  • Molecular formula

    C20H26N4O
  • Storage instructions

    Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months.
  • Solubility overview

    Soluble in DMSO to 100 mM
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILES

    CCCNC(=O)C(C#N)=Cc2cn(CCCN(C)C)c1ccccc12
  • Source

    Synthetic

  • Research areas

    • Biochemicals
    • Chemical Type
    • Biochemicals
    • Biochemicals
    • Pharmacology
    • Enzymes
    • ATPase & GTPase
    • Inhibitors
    • Biochemicals
    • Pharmacology
    • Signaling
    • Endocytosis & exocytosis
    • Dynamin
    • Biochemicals
    • Research Area
    • Heart disease
    • Signaling
    • Endocytosis & exocytosis
    • Dynamin
    • Biochemicals
    • Research Area
    • Pain & inflammation
    • Signaling
    • Endocytosis & exocytosis
    • Dynamin
    • Biochemicals
    • Research Area
    • Alzheimer's Disease
    • Signaling
    • Endocytosis & exocytosis
    • Dynamin
    • Biochemicals
    • Research Area
    • Diabetes
    • Signaling
    • Endocytosis & exocytosis
    • Dynamin
    • Biochemicals
    • Research Area
    • Hypertension
    • Signaling
    • Endocytosis & exocytosis
    • Dynamin
    • Biochemicals
    • Research Area
    • Obesity
    • Signaling
    • Endocytosis & exocytosis
    • Dynamin
    • Biochemicals
    • Research Area
    • Respiratory disease
    • Signaling
    • Endocytosis & exocytosis
    • Dynamin
    • Biochemicals
    • Research Area
    • Stroke
    • Signaling
    • Endocytosis & exocytosis
    • Dynamin

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab120464 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies
Use at an assay dependent concentration.
Notes
Functional Studies
Use at an assay dependent concentration.

Images

  • Chemical Structure - Dynole<sup>&reg;</sup> 31-2, Negative control for Dynole<sup>reg</sup> 34-2 (ab120464)
    Chemical Structure - Dynole® 31-2, Negative control for Dynolereg 34-2 (ab120464)
    2D chemical structure image of ab120464, Dynole® 31-2, Negative control for Dynolereg 34-2
  • Immunocytochemistry/ Immunofluorescence - Dynole® 31-2, Negative control for Dynolereg 34-2 (ab120464)
    Immunocytochemistry/ Immunofluorescence - Dynole® 31-2, Negative control for Dynolereg 34-2 (ab120464)
    ab66705 staining PAI1 in HeLa cells treated with dynole-31-2™ (ab120464), by ICC/IF. No change in PAI1 expression with increased concentration of dynole-31-2™ (negative control for dynole 34-2™ (ab120463), as described in literature.
    The cells were incubated at 37°C for 6h in media containing different concentrations of ab120464 (dynole-31-2™) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab66705 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
  • Functional Studies - Dynole<sup>&reg;</sup> 31-2, Negative control for Dynole<sup>reg</sup> 34-2 (ab120464)
    Functional Studies - Dynole® 31-2, Negative control for Dynolereg 34-2 (ab120464)Eppler et al PLoS One. 2017; 12(3): e0172443. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Dynamin2 regulates integrin-mediated T lymphocyte adhesion under laminar flow. 

    End point quantification of T lymphocyte adhesion following treatment of the cells with either Dynole 31–2 as a control or Dynole 34–2 to inhibit dynamin2 activity (n = 6)

    (From Figure 2D of Eppler et al).

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download
  • COA

References (7)

Publishing research using ab120464? Please let us know so that we can cite the reference in this datasheet.

ab120464 has been referenced in 7 publications.

  • Stallaert W  et al. Contact inhibitory Eph signaling suppresses EGF-promoted cell migration by decoupling EGFR activity from vesicular recycling. Sci Signal 11:N/A (2018). PubMed: 30065026
  • Eppler FJ  et al. Dynamin2 controls Rap1 activation and integrin clustering in human T lymphocyte adhesion. PLoS One 12:e0172443 (2017). PubMed: 28273099
  • González-Jamett AM  et al. Dynamin-2 mutations linked to Centronuclear Myopathy impair actin-dependent trafficking in muscle cells. Sci Rep 7:4580 (2017). PubMed: 28676641
  • Szymanska E  et al. Impaired dynamin 2 function leads to increased AP-1 transcriptional activity through the JNK/c-Jun pathway. Cell Signal 28:160-71 (2016). PubMed: 26475677
  • Krzyzaniak MA  et al. Host cell entry of respiratory syncytial virus involves macropinocytosis followed by proteolytic activation of the F protein. PLoS Pathog 9:e1003309 (2013). PubMed: 23593008
  • Richard JP  et al. Intracellular curvature-generating proteins in cell-to-cell fusion. Biochem J 440:185-93 (2011). PubMed: 21895608
  • de la Vega M  et al. Inhibition of HIV-1 endocytosis allows lipid mixing at the plasma membrane, but not complete fusion. Retrovirology 8:99 (2011). PubMed: 22145853

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