For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    products/biochemicals/dynole-34-2-dynamin-i-and-dynamin-ii-inhibitor-ab120463.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Signal Transduction Protein Trafficking Vesicle Transport Regulation
Share by email

Dynole® 34-2, dynamin I and dynamin II inhibitor (ab120463)

  • Datasheet
  • SDS
  • COA
Submit a review Submit a question References (11)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Chemical Structure - Dynole<sup>&reg;</sup> 34-2, dynamin I and dynamin II inhibitor (ab120463)
  • Immunocytochemistry/ Immunofluorescence - Dynole® 34-2, dynamin I and dynamin II inhibitor (ab120463)
  • Functional Studies - Dynole<sup>&reg;</sup> 34-2, dynamin I and dynamin II inhibitor (ab120463)

Key features and details

  • Potent dynamin I and dynamin II inhibitor
  • CAS Number: 1128165-88-7
  • Soluble in DMSO to 100 mM
  • Form / State: Solid
  • Source: Synthetic

You may also be interested in

Kit
Product image
Live Cell Labeling Kit - Red Fluorescence - Cytopainter (ab187965)
Primary
Product image
Anti-Cytosolic Phospholipase A2 antibody (ab73406)

View more associated products

Overview

  • Product name

    Dynole® 34-2, dynamin I and dynamin II inhibitor
  • Description

    Potent dynamin I and dynamin II inhibitor
  • Biological description

    Potent, cell-permeable dynamin I and dynamin II inhibitor (IC50 values are 1.3 and 14.2 μM for inhibition of dynamin I and II GTPase, respectively). Targets the GTPase domain at an allosteric site. Potently inhibits receptor-mediated and synaptic vesicle endocytosis (IC50 values are 5 and 41.1 μM, respectively). 15-fold more active than Dynasore (ab120192) against dynamin I.

  • General notes

    Sold under exclusive licence from Children's Medical Research Institute and Newcastle Innovation Ltd. Dynole® is a trademark of Children's Medical Research Institute and Newcastle Innovation Ltd.
  • CAS Number

    1128165-88-7
  • Chemical structure

    Chemical Structure

Properties

  • Chemical name

    2-Cyano-N-octyl-3-[1-(3-dimethylaminopropyl)-1H-indol-3-yl]acrylamide
  • Molecular weight

    408.59
  • Molecular formula

    C25H36N4O
  • PubChem identifier

    44157463
  • Storage instructions

    Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months.
  • Solubility overview

    Soluble in DMSO to 100 mM
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILES

    CCCCCCCCNC(=O)C(C#N)=Cc2cn(CCCN(C)C)c1ccccc12
  • Source

    Synthetic

  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Regulation
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Synapse marker
    • Biochemicals
    • Chemical Type
    • Biochemicals
    • Biochemicals
    • Pharmacology
    • Enzymes
    • ATPase & GTPase
    • Inhibitors
    • Biochemicals
    • Pharmacology
    • Signaling
    • Endocytosis & exocytosis
    • Dynamin
    • Biochemicals
    • Research Area
    • Heart disease
    • Signaling
    • Endocytosis & exocytosis
    • Dynamin
    • Biochemicals
    • Research Area
    • Pain & inflammation
    • Signaling
    • Endocytosis & exocytosis
    • Dynamin
    • Biochemicals
    • Research Area
    • Alzheimer's Disease
    • Signaling
    • Endocytosis & exocytosis
    • Dynamin
    • Biochemicals
    • Research Area
    • Diabetes
    • Signaling
    • Endocytosis & exocytosis
    • Dynamin
    • Biochemicals
    • Research Area
    • Hypertension
    • Signaling
    • Endocytosis & exocytosis
    • Dynamin
    • Biochemicals
    • Research Area
    • Obesity
    • Signaling
    • Endocytosis & exocytosis
    • Dynamin
    • Biochemicals
    • Research Area
    • Respiratory disease
    • Signaling
    • Endocytosis & exocytosis
    • Dynamin
    • Biochemicals
    • Research Area
    • Stroke
    • Signaling
    • Endocytosis & exocytosis
    • Dynamin

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab120463 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies
Use at an assay dependent concentration.
Notes
Functional Studies
Use at an assay dependent concentration.

Images

  • Chemical Structure - Dynole<sup>&reg;</sup> 34-2, dynamin I and dynamin II inhibitor (ab120463)
    Chemical Structure - Dynole® 34-2, dynamin I and dynamin II inhibitor (ab120463)
    2D chemical structure image of ab120463, Dynole® 34-2, dynamin I and dynamin II inhibitor
  • Immunocytochemistry/ Immunofluorescence - Dynole® 34-2, dynamin I and dynamin II inhibitor (ab120463)
    Immunocytochemistry/ Immunofluorescence - Dynole® 34-2, dynamin I and dynamin II inhibitor (ab120463)

    ab66705 staining PAI1 in HeLa cells treated with dynole-34-2™; (ab120463), by ICC/IF. Increase in PAI1 expression correlates with increased concentration of dynole-34-2™, as described in literature.
    The cells were incubated at 37°C for 24h in media containing different concentrations of ab120463 (dynole-34-2™) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab66705 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

  • Functional Studies - Dynole<sup>&reg;</sup> 34-2, dynamin I and dynamin II inhibitor (ab120463)
    Functional Studies - Dynole® 34-2, dynamin I and dynamin II inhibitor (ab120463)Image from Kockx et al., PLOS One., 9(10): e111186. Fig 1F.; doi: https://doi.org/10.1371/journal.pone.0111186. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Primary human monocyte-derived macrophages (HMDM) were incubated with 30 µM of ab120463 for indicated time periods to determine if Dynamin inhibition decreases apoE secretion from primary human macrophages. Dynole-34-2 both dose- and time-dependently decreased apoE secretion without affecting HMDM viability (botton line on the graph as appose to the control, top line).

    Kockx et al., PLOS One., 9(10): e111186. Fig 1F.; doi: https://doi.org/10.1371/journal.pone.0111186.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download
  • COA

References (11)

Publishing research using ab120463? Please let us know so that we can cite the reference in this datasheet.

ab120463 has been referenced in 11 publications.

  • Lynn KS  et al. Asymmetric distribution of dynamin-2 and ß-catenin relative to tight junction spikes in alveolar epithelial cells. Tissue Barriers N/A:1929786 (2021). PubMed: 34107845
  • Štimac I  et al. Dynamin Inhibitors Prevent the Establishment of the Cytomegalovirus Assembly Compartment in the Early Phase of Infection. Life (Basel) 11:N/A (2021). PubMed: 34575026
  • Ivanova D  et al. CtBP1-Mediated Membrane Fission Contributes to Effective Recycling of Synaptic Vesicles. Cell Rep 30:2444-2459.e7 (2020). PubMed: 32075774
  • Wang D  et al. ERK is involved in the differentiation and function of dimethyl sulfoxide-induced HL-60 neutrophil-like cells, which mimic inflammatory neutrophils. Int Immunopharmacol 84:106510 (2020). PubMed: 32361568
  • Stallaert W  et al. Contact inhibitory Eph signaling suppresses EGF-promoted cell migration by decoupling EGFR activity from vesicular recycling. Sci Signal 11:N/A (2018). PubMed: 30065026
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab120463.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES, NOT FOR USE IN HUMANS"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2023 Abcam plc. All rights reserved.