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    products/biochemicals/fccp-mitochondrial-oxidative-phosphorylation-uncoupler-ab120081.pdf

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Biochemicals Chemical Type Biochemicals
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FCCP, mitochondrial oxidative phosphorylation uncoupler (ab120081)

  • Datasheet
  • SDS
  • COA
Reviews (1)Q&A (1)References (33)

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Chemical Structure - FCCP, mitochondrial oxidative phosphorylation uncoupler (ab120081)
  • Cellular activation - FCCP, mitochondrial oxidative phosphorylation uncoupler (ab120081)

Key features and details

  • Potent mitochondrial oxidative phosphorylation uncoupler
  • CAS Number: 370-86-5
  • Soluble in DMSO to 100 mM
  • Form / State: Solid
  • Source: Synthetic

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Overview

  • Product name

    FCCP, mitochondrial oxidative phosphorylation uncoupler
  • Description

    Potent mitochondrial oxidative phosphorylation uncoupler
  • Biological description

    Potent mitochondrial oxidative phosphorylation uncoupler (IC50 = 20 nM). Disrupts ATP synthesis by transporting protons across mitochondrial inner membranes. Depolarises mitochondrial membrane potential.

  • CAS Number

    370-86-5
  • Chemical structure

    Chemical Structure

Properties

  • Chemical name

    Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone
  • Molecular weight

    254.17
  • Molecular formula

    C10H5F3N4O
  • PubChem identifier

    330
  • Storage instructions

    Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months.
  • Solubility overview

    Soluble in DMSO to 100 mM
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Toxic, refer to SDS for further information.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILES

    FC(F)(F)Oc1ccc(cc1)NN=C(/C#N)C#N
  • Source

    Synthetic

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Associated products

  • Alternative Versions

    • FCCP, mitochondrial oxidative phosphorylation uncoupler (DMSO solution) (ab147482)

Images

  • Chemical Structure - FCCP, mitochondrial oxidative phosphorylation uncoupler (ab120081)
    Chemical Structure - FCCP, mitochondrial oxidative phosphorylation uncoupler (ab120081)
    2D chemical structure image of ab120081, FCCP, mitochondrial oxidative phosphorylation uncoupler
  • Cellular activation - FCCP, mitochondrial oxidative phosphorylation uncoupler (ab120081)
    Cellular activation - FCCP, mitochondrial oxidative phosphorylation uncoupler (ab120081)Image from Chan G K Y, et al. Plos One, 8(5), e63583. Fig S4,; doi: 10.1371/journal.pone.0063583
    HT29 cells were treated with the indicated compounds ((etoposide, 10 µM; gemcitabine 0.1 µM; paclitaxel 0.01 µM; PD901 1 µM, VX-680 0.2 µM) for 24 hours before analysis of oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) using the Seahorse XF96 extracellular flux analyzer. Baseline rates (black) were determined at the indicated times before the addition of oligomycin (green) and then FCCP (red). Rate data are normalized to per-well cell number determined by post-analysis high-content imaging.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download
  • COA

References (33)

Publishing research using ab120081? Please let us know so that we can cite the reference in this datasheet.

ab120081 has been referenced in 33 publications.

  • Blancke Soares A  et al. High-resolution spatiotemporal pHe and pO2 imaging in head and neck and oesophageal carcinoma cells. Cancer Metab 9:21 (2021). PubMed: 33947450
  • Crivellari I  et al. Impaired mitochondrial quality control in Rett Syndrome. Arch Biochem Biophys 700:108790 (2021). PubMed: 33549528
  • Duong A  et al. Characterization of mitochondrial health from human peripheral blood mononuclear cells to cerebral organoids derived from induced pluripotent stem cells. Sci Rep 11:4523 (2021). PubMed: 33633238
  • Mazzoleni V  et al. Staphylococcus aureus Panton-Valentine Leukocidin triggers an alternative NETosis process targeting mitochondria. FASEB J 35:e21167 (2021). PubMed: 33241563
  • Moya ELJ  et al. Miniaturization and Automation of a Human In Vitro Blood-Brain Barrier Model for the High-Throughput Screening of Compounds in the Early Stage of Drug Discovery. Pharmaceutics 13:N/A (2021). PubMed: 34208550
View all Publications for this product

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Good uncoupler of oxidative phosphorylation in mitochondria

Good
Abreviews
Abreviews
abreview image
We obtained the expected results with a maximum respiration higher than the basal respiration.
Disadvantages: Low stability once in solution (1 month at -20ºC according to abcam).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 06 2020

Question

I chose 10uM FCCP because in your protocol given it says to use between 2-10UM of CCCP or FCCP. I decided to try 10uM because I have previously done JC-1 experiments with an Invitrogen kit and they recommended a concentration of 50uM so I just chose the highest concentration you suggested...

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Abcam community

Verified customer

Asked on Dec 17 2012

Answer



The concentration of CCCP or FCCP required as a positive control will vary based on cell line. Perhaps it would be useful to try 50uM FCCP as this has worked for you in the past.

Read More

Abcam Scientific Support

Answered on Dec 17 2012

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