Fenofibrate, PPAR-alpha agonist (ab120832)
Key features and details
- Potent, selective PPAR-α agonist
- CAS Number: 49562-28-9
- Purity: > 99%
- Soluble in DMSO to 100 mM and in ethanol to 100 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
Fenofibrate, PPAR-alpha agonist -
Description
Potent, selective PPAR-α agonist -
Biological description
Potent and selective PPAR-α agonist (EC50 values are 18 and 30 μM at mouse and human receptors, respectively). Affinity is 10-fold less at PPAR-γ (EC50 values are 250 and 300 μM at mouse and human receptors, respectively). Hypolipidemic agent.
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Purity
> 99% -
CAS Number
49562-28-9 -
Chemical structure
Properties
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Chemical name
2-[4-(4-Chlorobenzoyl)phenoxy]-2-methylpropanoic acid isopropyl ester -
Molecular weight
360.84 -
Molecular formula
C20H21ClO4 -
Storage instructions
Store at Room Temperature. The product can be stored for up to 12 months. -
Solubility overview
Soluble in DMSO to 100 mM and in ethanol to 100 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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Source
Synthetic
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Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab120832 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| Notes |
|---|
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Functional Studies
Use at an assay dependent concentration. |
Images
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Chemical Structure - Fenofibrate, PPAR-alpha agonist (ab120832)2D chemical structure image of ab120832, Fenofibrate, PPAR-alpha agonist
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Western blot - Fenofibrate, PPAR-alpha agonist (ab120832)HL-60 cells were incubated at 37°C for 24h with vehicle control (0 µM) and different concentrations of fenofibrate (ab120832). Increased expression of ACADM in HL-60 cells correlates with an increase in fenofibrate concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10 µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab110296 at 1 µg/ml and ab9484 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP (ab97040) at 1/10000 dilution and visualised using ECL development solution.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (3)
ab120832 has been referenced in 3 publications.
- Zhou T et al. Fenofibrate suppresses corneal neovascularization by regulating lipid metabolism through PPARα signaling pathway. Front Pharmacol 13:1000254 (2022). PubMed: 36588740
- Takahashi H et al. TGF-ß2 is an exercise-induced adipokine that regulates glucose and fatty acid metabolism. Nat Metab 1:291-303 (2019). PubMed: 31032475
- Wang Z et al. Naoxintong Retards Atherosclerosis by Inhibiting Foam Cell Formation Through Activating Ppara Pathway. Curr Mol Med 18:698-710 (2018). PubMed: 30734676